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Protein

2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene

menH

Organism
Enterobacter sp. (strain 638)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes a proton abstraction reaction that results in 2,5-elimination of pyruvate from 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) and the formation of 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC).UniRule annotation

Catalytic activityi

5-enolpyruvoyl-6-hydroxy-2-succinylcyclohex-3-ene-1-carboxylate = (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate + pyruvate.UniRule annotation

Pathwayi: 1,4-dihydroxy-2-naphthoate biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes 1,4-dihydroxy-2-naphthoate from chorismate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD)
  3. 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (menH)
  4. o-succinylbenzoate synthase (menC)
  5. no protein annotated in this organism
  6. 1,4-dihydroxy-2-naphthoyl-CoA synthase (menB)
  7. 1,4-dihydroxy-2-naphthoyl-CoA hydrolase (menI)
This subpathway is part of the pathway 1,4-dihydroxy-2-naphthoate biosynthesis, which is itself part of Quinol/quinone metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 1,4-dihydroxy-2-naphthoate from chorismate, the pathway 1,4-dihydroxy-2-naphthoate biosynthesis and in Quinol/quinone metabolism.

Pathwayi: menaquinone biosynthesis

This protein is involved in the pathway menaquinone biosynthesis, which is part of Quinol/quinone metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway menaquinone biosynthesis and in Quinol/quinone metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Menaquinone biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00079.
UPA01057; UER00900.

Protein family/group databases

ESTHERient38-menh. MenH_SHCHC.
MEROPSiS33.996.

Names & Taxonomyi

Protein namesi
Recommended name:
2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthaseUniRule annotation (EC:4.2.99.20UniRule annotation)
Short name:
SHCHC synthaseUniRule annotation
Gene namesi
Name:menHUniRule annotation
Ordered Locus Names:Ent638_2813
OrganismiEnterobacter sp. (strain 638)
Taxonomic identifieri399742 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEnterobacter
Proteomesi
  • UP000000230 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003419031 – 2582-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthaseAdd BLAST258

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi399742.Ent638_2813.

Structurei

3D structure databases

ProteinModelPortaliA4WCP8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. MenH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107J5P. Bacteria.
COG0596. LUCA.
HOGENOMiHOG000028072.
KOiK08680.
OMAiLNDWYQQ.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01660. MenH. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR022485. SHCHC_synthase_MenH.
[Graphical view]
PfamiPF12697. Abhydrolase_6. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR03695. menH_SHCHC. 1 hit.

Sequencei

Sequence statusi: Complete.

A4WCP8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILNGKTRAG KPGFPWLVFL HGFSGDSREW QDVGAQLPDY PQLHLDLPGH
60 70 80 90 100
GDSANIAVAG FDEVSQLLAD TLVSYNILKF WLVGYSLGGR IAMFHACQHP
110 120 130 140 150
AGLIGLIVEG GHPGLHDEPT RKARAESDAQ WAERFRREPL ENVFSAWYQQ
160 170 180 190 200
PVFAALQETE RDALVALRSQ NNGAALGAML QATSLAQQPD LRAALRAHDY
210 220 230 240 250
PFHYVYGEHD DKFGAIATEL TAQRHVIPNA GHNAHRENPA AVAACLAQIL

RLRIKDIP
Length:258
Mass (Da):28,379
Last modified:May 29, 2007 - v1
Checksum:iC812BBA2C7F469C1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000653 Genomic DNA. Translation: ABP61478.1.
RefSeqiWP_015959811.1. NC_009436.1.

Genome annotation databases

EnsemblBacteriaiABP61478; ABP61478; Ent638_2813.
KEGGient:Ent638_2813.
PATRICi20415289. VBIEntSp101211_2963.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000653 Genomic DNA. Translation: ABP61478.1.
RefSeqiWP_015959811.1. NC_009436.1.

3D structure databases

ProteinModelPortaliA4WCP8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi399742.Ent638_2813.

Protein family/group databases

ESTHERient38-menh. MenH_SHCHC.
MEROPSiS33.996.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABP61478; ABP61478; Ent638_2813.
KEGGient:Ent638_2813.
PATRICi20415289. VBIEntSp101211_2963.

Phylogenomic databases

eggNOGiENOG4107J5P. Bacteria.
COG0596. LUCA.
HOGENOMiHOG000028072.
KOiK08680.
OMAiLNDWYQQ.

Enzyme and pathway databases

UniPathwayiUPA00079.
UPA01057; UER00900.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01660. MenH. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR022485. SHCHC_synthase_MenH.
[Graphical view]
PfamiPF12697. Abhydrolase_6. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR03695. menH_SHCHC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMENH_ENT38
AccessioniPrimary (citable) accession number: A4WCP8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: May 29, 2007
Last modified: November 2, 2016
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.