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A4WBR1

- OTSA_ENT38

UniProt

A4WBR1 - OTSA_ENT38

Protein

Alpha,alpha-trehalose-phosphate synthase [UDP-forming]

Gene

otsA

Organism
Enterobacter sp. (strain 638)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 48 (01 Oct 2014)
      Sequence version 1 (29 May 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor By similarity.By similarity

    Catalytic activityi

    UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei10 – 101Glucose-6-phosphateBy similarity
    Binding sitei77 – 771Glucose-6-phosphateBy similarity
    Sitei86 – 861Involved in alpha anomer selectivityBy similarity
    Binding sitei131 – 1311Glucose-6-phosphateBy similarity
    Sitei156 – 1561Involved in alpha anomer selectivityBy similarity
    Binding sitei263 – 2631UDP-glucoseBy similarity
    Binding sitei268 – 2681UDP-glucoseBy similarity
    Binding sitei301 – 3011Glucose-6-phosphateBy similarity
    Binding sitei340 – 3401UDP-glucose; via amide nitrogen and carbonyl oxygenBy similarity

    GO - Molecular functioni

    1. alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity Source: UniProtKB-EC

    GO - Biological processi

    1. trehalose biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciESP399742:GJ0E-2520-MONOMER.
    RETL1328306-WGS:GSTH-488-MONOMER.
    UniPathwayiUPA00299.

    Protein family/group databases

    CAZyiGT20. Glycosyltransferase Family 20.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha,alpha-trehalose-phosphate synthase [UDP-forming] (EC:2.4.1.15)
    Alternative name(s):
    Osmoregulatory trehalose synthesis protein A
    Trehalose-6-phosphate synthase
    UDP-glucose-glucosephosphate glucosyltransferase
    Gene namesi
    Name:otsA
    Ordered Locus Names:Ent638_2472
    OrganismiEnterobacter sp. (strain 638)
    Taxonomic identifieri399742 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacter
    ProteomesiUP000000230: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 474474Alpha,alpha-trehalose-phosphate synthase [UDP-forming]PRO_0000348892Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi399742.Ent638_2472.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni22 – 232UDP-glucose bindingBy similarity
    Regioni366 – 3705UDP-glucose bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyltransferase 20 family.Curated

    Phylogenomic databases

    eggNOGiCOG0380.
    HOGENOMiHOG000191478.
    KOiK00697.
    OMAiWGESFVK.
    OrthoDBiEOG6CCH1M.

    Family and domain databases

    InterProiIPR001830. Glyco_trans_20.
    IPR012766. Trehalose_OtsA.
    [Graphical view]
    PfamiPF00982. Glyco_transf_20. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02400. trehalose_OtsA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A4WBR1-1 [UniParc]FASTAAdd to Basket

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    MGRLVVVSNR IAPPDDKKSS AGGLAVGILG ALKTAGGLWF GWSGEVGNED    50
    KPLQKVTRGN ITWASFNLSE QDHEEYYSQF SNAVLWPAFH YRLDLVKFQR 100
    DSWEGYTRVN ALLADKLLPL IEEDDILWIH DYHLLPFASE LRKRGVNNRI 150
    GFFLHIPFPT PEIFTAIPPH EELLEGLCDY DLLGFQTEND RQAFLESVAG 200
    KTRLTTHNGK SHQAWGKTFD TEVYPIGIEP DEIAADASGP LPPKLAQLKN 250
    ELKNVKNIFS VERLDYSKGL PERFQAYERL LEKYPQHHGK IRYTQIAPTS 300
    RGEVQAYQDI RHQLETEAGR INGRYGQLGW TPLYYLNQHF ERKVLMKVFR 350
    YAEVGLVTPL RDGMNLVAKE YVAAQDPKDP GVLVLSQFAG AANELTSALL 400
    VNPYDSDDVA NALDRALKMP LTERISRHAE MMKVIRENDI NHWQETFIRD 450
    LKRTTPRSVE SNLQKKIATF PKLA 474
    Length:474
    Mass (Da):53,911
    Last modified:May 29, 2007 - v1
    Checksum:i58A0FA8BED879316
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000653 Genomic DNA. Translation: ABP61141.1.
    RefSeqiWP_015959474.1. NC_009436.1.
    YP_001177192.1. NC_009436.1.

    Genome annotation databases

    EnsemblBacteriaiABP61141; ABP61141; Ent638_2472.
    GeneIDi5111632.
    KEGGient:Ent638_2472.
    PATRICi20414581. VBIEntSp101211_2618.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000653 Genomic DNA. Translation: ABP61141.1 .
    RefSeqi WP_015959474.1. NC_009436.1.
    YP_001177192.1. NC_009436.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 399742.Ent638_2472.

    Protein family/group databases

    CAZyi GT20. Glycosyltransferase Family 20.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABP61141 ; ABP61141 ; Ent638_2472 .
    GeneIDi 5111632.
    KEGGi ent:Ent638_2472.
    PATRICi 20414581. VBIEntSp101211_2618.

    Phylogenomic databases

    eggNOGi COG0380.
    HOGENOMi HOG000191478.
    KOi K00697.
    OMAi WGESFVK.
    OrthoDBi EOG6CCH1M.

    Enzyme and pathway databases

    UniPathwayi UPA00299 .
    BioCyci ESP399742:GJ0E-2520-MONOMER.
    RETL1328306-WGS:GSTH-488-MONOMER.

    Family and domain databases

    InterProi IPR001830. Glyco_trans_20.
    IPR012766. Trehalose_OtsA.
    [Graphical view ]
    Pfami PF00982. Glyco_transf_20. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02400. trehalose_OtsA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the plant growth promoting endophytic bacterium Enterobacter sp. 638."
      Taghavi S., van der Lelie D., Hoffman A., Zhang Y.B., Walla M.D., Vangronsveld J., Newman L., Monchy S.
      PLoS Genet. 6:E1000943-E1000943(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 638.

    Entry informationi

    Entry nameiOTSA_ENT38
    AccessioniPrimary (citable) accession number: A4WBR1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 2, 2008
    Last sequence update: May 29, 2007
    Last modified: October 1, 2014
    This is version 48 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3