Reviewed, UniProtKB/Swiss-Prot A4WBF2 (DADA_ENT38)

Names and origin

Protein names

Recommended name:
    D-amino acid dehydrogenase small subunit
    EC=1.4.99.1

Gene names

Name:	dadA
Ordered Locus Names:	Ent638_2363

Organism

Enterobacter sp. (strain 638) [Complete proteome] [HAMAP]

Taxonomic identifier

399742 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Enterobacter

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Oxidative deamination of D-amino acids By similarity.
Catalytic activity	A D-amino acid + H2O + acceptor = a 2-oxo acid + NH3 + reduced acceptor. HAMAP MF_01202
Cofactor	FAD By similarity.
Pathway	Amino-acid degradation; D-alanine degradation; NH(3) and pyruvate from D-alanine: step 1/1. HAMAP MF_01202
Subunit structure	Heterodimer of a small and a large subunit By similarity.
Sequence similarities	Belongs to the dadA oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	D-amino-acid dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 432	432	D-amino acid dehydrogenase small subunit HAMAP MF_01202		PRO_1000066093
Regions
	Nucleotide binding	3 – 17	15	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	A4WBF2-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: A2499274FEC70BC6	FASTA	432	47,363
	10 20 30 40 50 60 MRVVVLGSGV VGVTSAWYLC QAGHEVTVID REPGSALETS AANAGQISPG YAAPWAAPGV 70 80 90 100 110 120 PLKAIKWMFQ RHAPLAISLD GTPSQLKWMW QMLRNCDTRH YMENKGRMVR LAEYSRDCLK 130 140 150 160 170 180 TLRASTGIEY EGRQGGTLQL FRTAQQYENA TRDIAVLEDA GVPYQLLDAS QLAQVEPALA 190 200 210 220 230 240 EVAHKLTGGL RLPNDETGDC QLFTQRLAKM CEQAGVTFRY NTPVDKLLSE GGKIYGVKCG 250 260 270 280 290 300 DEVIKADSYV MAFGSYSTAM LKGLLDIPVY PLKGYSLTIP VKEDNGAPVS TILDETYKIA 310 320 330 340 350 360 ITRFDNRIRV GGMAEIVGFN TELLQPRRET LEMVVRDLFP RGGFIEQATF WTGLRPMTPD 370 380 390 400 410 420 GTPIVGRTPF KNLWTNTGHG TLGWTMACGS GQLLSDLISG RTPAIPFDDL SAARYQSGFS 430 PSRPQHLHGA HN

References

[1]

"Complete sequence of chromosome of Enterobacter sp. 638." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Taghavi S., Newman L., Vangronsveld J., van der Lelie D., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000653 Genomic DNA. Translation: ABP61032.1.
RefSeq	YP_001177083.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5111027.
GenomeReviews	Gene locus Ent638_2363 in contig CP000653_GR.
KEGG	ent:Ent638_2363.
NMPDR	fig|399742.4.peg.2272.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A4WBF2. MFQKHAP.
Family and domain databases
HAMAP	MF_01202. [Tree]
InterPro	IPR006076. FAD-dep_OxRdtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01266. DAO. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DADA_ENT38

Accession

Primary (citable) accession number: A4WBF2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	May 29, 2007
Last modified:	June 16, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)