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A4WAY2 (PYRF_ENT38) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Orotidine 5'-phosphate decarboxylase

EC=4.1.1.23
Alternative name(s):
OMP decarboxylase
Short name=OMPDCase
Short name=OMPdecase
Gene names
Name:pyrF
Ordered Locus Names:Ent638_2187
OrganismEnterobacter sp. (strain 638) [Complete proteome] [HAMAP]
Taxonomic identifier399742 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacter

Protein attributes

Sequence length246 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the decarboxylation of orotidine 5'-monophosphate (OMP) to uridine 5'-monophosphate (UMP) By similarity. HAMAP-Rule MF_01200

Catalytic activity

Orotidine 5'-phosphate = UMP + CO2. HAMAP-Rule MF_01200

Pathway

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2. HAMAP-Rule MF_01200

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01200

Sequence similarities

Belongs to the OMP decarboxylase family. Type 1 subfamily.

Ontologies

Keywords
   Biological processPyrimidine biosynthesis
   Molecular functionDecarboxylase
Lyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_process'de novo' UMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

'de novo' pyrimidine nucleobase biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionorotidine-5'-phosphate decarboxylase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 246246Orotidine 5'-phosphate decarboxylase HAMAP-Rule MF_01200
PRO_1000065908

Regions

Region71 – 8010Substrate binding By similarity

Sites

Active site731Proton donor By similarity
Binding site221Substrate By similarity
Binding site441Substrate By similarity
Binding site1311Substrate By similarity
Binding site1921Substrate By similarity
Binding site2011Substrate By similarity
Binding site2211Substrate; via amide nitrogen By similarity
Binding site2221Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
A4WAY2 [UniParc].

Last modified May 29, 2007. Version 1.
Checksum: A57DEE9B1D5FDB6E

FASTA24626,155
        10         20         30         40         50         60 
MTSVASSSSR IVTESPVVVA LDYNNRDSAL AFVDLIDPRD CRLKVGKEMF TLFGPQIVRD 

        70         80         90        100        110        120 
LQQRGFDVFL DLKFHDIPNT TAHAVAAAAD LGVWMVNVHA SGGARMMTAA REALIPFGKD 

       130        140        150        160        170        180 
APLLIAVTVL TSMEASDLLD LGVTLSPAEH AERLARLTQN CGLDGVVCSA QEAVRFKSAL 

       190        200        210        220        230        240 
GHNFKLVTPG IRPQGSDAGD QRRIMTPEEA LAAGVDYMVI GRPVTQSADP ALTLKAINAS 


LGKVTG 

« Hide

References

[1]"Genome sequence of the plant growth promoting endophytic bacterium Enterobacter sp. 638."
Taghavi S., van der Lelie D., Hoffman A., Zhang Y.B., Walla M.D., Vangronsveld J., Newman L., Monchy S.
PLoS Genet. 6:E1000943-E1000943(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 638.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000653 Genomic DNA. Translation: ABP60862.1.
RefSeqYP_001176913.1. NC_009436.1.

3D structure databases

ProteinModelPortalA4WAY2.
SMRA4WAY2. Positions 12-243.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING399742.Ent638_2187.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABP60862; ABP60862; Ent638_2187.
GeneID5112879.
KEGGent:Ent638_2187.
PATRIC20414021. VBIEntSp101211_2340.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0284.
HOGENOMHOG000226071.
KOK01591.
OMARPITQSA.
OrthoDBEOG6N6815.

Enzyme and pathway databases

BioCycESP399742:GJ0E-2233-MONOMER.
UniPathwayUPA00070; UER00120.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01200_B. OMPdecase_type1_B.
InterProIPR013785. Aldolase_TIM.
IPR014732. OMPdecase.
IPR018089. OMPdecase_AS.
IPR001754. OMPdeCOase_dom.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamPF00215. OMPdecase. 1 hit.
[Graphical view]
SMARTSM00934. OMPdecase. 1 hit.
[Graphical view]
SUPFAMSSF51366. SSF51366. 1 hit.
TIGRFAMsTIGR01740. pyrF. 1 hit.
PROSITEPS00156. OMPDECASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePYRF_ENT38
AccessionPrimary (citable) accession number: A4WAY2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: May 29, 2007
Last modified: May 14, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways