Reviewed, UniProtKB/Swiss-Prot A4WAR9 (ABDH_ENT38)

Names and origin

Protein names

Recommended name:
    Gamma-aminobutyraldehyde dehydrogenase
    EC=1.2.1.19
Alternative name(s):
    1-pyrroline dehydrogenase
    4-aminobutanal dehydrogenase
      Short name=ABALDH

Gene names

Ordered Locus Names:

Ent638_2124

Organism

Enterobacter sp. (strain 638) [Complete proteome] [HAMAP]

Taxonomic identifier

399742 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Enterobacter

Protein attributes

Sequence length	474 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of 1-pyrroline, which is spontaneously formed from 4-aminobutanal, leading to 4-aminobutanoate (GABA) By similarity.
Catalytic activity	4-aminobutanal + NAD+ + H2O = 4-aminobutanoate + NADH. HAMAP MF_01275
Pathway	Amine and polyamine degradation; putrescine degradation; 4-aminobutanoate from 4-aminobutanal: step 1/1. HAMAP MF_01275
Subunit structure	Homotetramer By similarity.
Miscellaneous	4-aminobutanal is also called gamma-aminobutyraldehyde. HAMAP MF_01275
Sequence similarities	Belongs to the aldehyde dehydrogenase family. Gamma-aminobutyraldehyde dehydrogenase subfamily.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW putrescine catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: HAMAP aminobutyraldehyde dehydrogenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the CH-NH group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 474	474	Gamma-aminobutyraldehyde dehydrogenase HAMAP MF_01275		PRO_1000067394
Regions
	Nucleotide binding	172 – 175	4	NAD By similarity
	Nucleotide binding	225 – 231	7	NAD By similarity
Sites
	Active site	246	1	By similarity
	Active site	280	1	Nucleophile By similarity
	Binding site	146	1	NAD; via carbonyl oxygen By similarity
	Binding site	209	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4WAR9-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 46C332B65B30D601	FASTA	474	50,905
	10 20 30 40 50 60 MQNQLLINGE LVNGEGEKQP VYNPATGEVL LEIAEASAAQ VDAAVRAADR AFSEWGHTTP 70 80 90 100 110 120 KARAECLLKL ADVIEEHAET FARLESQNCG KPLHCVISDE IPAVVDVFRF FAGAARCLNG 130 140 150 160 170 180 LAAGEYLEGH TSMIRRDPVG VVASIAPWNY PLMMAAWKLA PALAAGNCVV IKPSEITPLT 190 200 210 220 230 240 ALKLAELAKD IFPAGVLNVL FGRGKTVGDP LTGHEKVRMV SLTGSIATGE HIISHTASSI 250 260 270 280 290 300 KRTHMELGGK APVIVFDDAD IDAVVEGVRT FGFYNAGQDC TAACRIYAQK GIYPQLVEKL 310 320 330 340 350 360 GAAVATLKTG APEDESTELG PLSSAAHLER VCKAVDEAKA LGHVNVVTGG KKVEGGGYFF 370 380 390 400 410 420 EPTLLAGAKQ EDAIVQREVF GPVVSMTEFD DEEQVLAWAN DSQYGLASSV WTQDVGRAHR 430 440 450 460 470 MSARLQYGCT WVNTHFMLVS EMPHGGMKLS GYGKDMSVYG LEDYTVIRHV MIKH

References

[1]

"Complete sequence of chromosome of Enterobacter sp. 638." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Taghavi S., Newman L., Vangronsveld J., van der Lelie D., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000653 Genomic DNA. Translation: ABP60799.1.
RefSeq	YP_001176850.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5113705.
GenomeReviews	Gene locus Ent638_2124 in contig CP000653_GR.
KEGG	ent:Ent638_2124.
NMPDR	fig|399742.4.peg.2072.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A4WAR9. QVLRWAN.
Family and domain databases
HAMAP	MF_01275. [Tree]
InterPro	IPR017749. 1-pyrroline_dehydrogenase. IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR03374. ABALDH. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. False negative. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ABDH_ENT38

Accession

Primary (citable) accession number: A4WAR9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	May 29, 2007
Last modified:	June 16, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)