A4WAM2 (ARND_ENT38) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnD
EC=3.5.1.n3

Gene names

Name:	arnD
Ordered Locus Names:	Ent638_2076

Organism

Enterobacter sp. (strain 638) [Complete proteome] [HAMAP]

Taxonomic identifier

399742 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Enterobacter

Protein attributes

Sequence length	298 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the deformylation of 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol to 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol. The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides By similarity. HAMAP-Rule MF_01870
Catalytic activity	4-deoxy-4-formamido-beta-L-arabinose di-trans,poly-cis-undecaprenyl phosphate + H2O = 4-amino-4-deoxy-alpha-L-arabinose di-trans,poly-cis-undecaprenyl phosphate + formate. HAMAP-Rule MF_01870
Pathway	Glycolipid biosynthesis; 4-amino-4-deoxy-alpha-L-arabinose undecaprenyl phosphate biosynthesis; 4-amino-4-deoxy-alpha-L-arabinose undecaprenyl phosphate from UDP-4-deoxy-4-formamido-beta-L-arabinose and undecaprenyl phosphate: step 2/2. HAMAP-Rule MF_01870 Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. HAMAP-Rule MF_01870
Sequence similarities	Belongs to the polysaccharide deacetylase family. ArnD deformylase subfamily.

Ontologies

Keywords
Biological process	Antibiotic resistance Lipid A biosynthesis Lipid biosynthesis Lipid metabolism Lipopolysaccharide biosynthesis
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	4-amino-4-deoxy-alpha-L-arabinopyranosyl undecaprenyl phosphate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway lipid A biosynthetic process Inferred from electronic annotation. Source: HAMAP lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 298	298	Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnD HAMAP-Rule MF_01870		PRO_0000383492

Sequences

	Sequence		Length	Mass (Da)
	A4WAM2 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: CA43E1E6B37E9373	FASTA	298	33,209
	10 20 30 40 50 60 MRKVGLRIDV DTWRGTQQGV PHLLEILSLH GIQATFFVSV GPDNMGRHLW RLVKPQFLWK 70 80 90 100 110 120 ILRSKAASLY GWDILLAGTA WPGRQIGAGN ADIISAAADH HEVGLHAWDH FAWQTWAGVW 130 140 150 160 170 180 DNAHLEKHIR LGLDSLSQII EQPVTCSAVA GWRADQRVVK AKESFGFRYN SDCRGTTPFR 190 200 210 220 230 240 PVLADGSTGT VQIPVTLPTW DEVIGEGVSR ENYNRFILDR IKQDTGTPVY TIHAEVEGIV 250 260 270 280 290 MRKHFSELLE MAAQEGITFC PLSELLPDDL STLPLGKVVR GAIPGREGWL GCQQLIDH

References

[1]

"Complete sequence of chromosome of Enterobacter sp. 638." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Taghavi S., Newman L., Vangronsveld J., van der Lelie D., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 638.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000653 Genomic DNA. Translation: ABP60752.1.
RefSeq	YP_001176803.1. NC_009436.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	399742.Ent638_2076.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABP60752; ABP60752; Ent638_2076.
GeneID	5112197.
KEGG	ent:Ent638_2076.
PATRIC	20413795. VBIEntSp101211_2227.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0726.
HOGENOM	HOG000261199.
KO	K13014.
OMA	HHGWQAN.
ProtClustDB	PRK15394.
Enzyme and pathway databases
BioCyc	ESP399742:GJ0E-2122-MONOMER.
UniPathway	UPA00030. UPA00036; UER00496.
Family and domain databases
Gene3D	3.20.20.370. 2 hits.
HAMAP	MF_01870. ArnD.
InterPro	IPR023557. ArnD. IPR011330. Glyco_hydro/deAcase_b/a-brl. IPR002509. Polysac_deacetylase. [Graphical view]
Pfam	PF01522. Polysacc_deac_1. 1 hit. [Graphical view]
SUPFAM	SSF88713. Glyco_hydro/deAcase_b/a-brl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARND_ENT38

Accession

Primary (citable) accession number: A4WAM2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	May 29, 2007
Last modified:	May 1, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program