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A4WAM1

- ARNT_ENT38

UniProt

A4WAM1 - ARNT_ENT38

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Protein
Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase
Gene
arnT, Ent638_2075
Organism
Enterobacter sp. (strain 638)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the transfer of the L-Ara4N moiety of the glycolipid undecaprenyl phosphate-alpha-L-Ara4N to lipid A. The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides By similarity.UniRule annotation

Catalytic activityi

4-amino-4-deoxy-alpha-L-arabinopyranosyl di-trans,octa-cis-undecaprenyl phosphate + lipid IV(A) = lipid II(A) + di-trans,octa-cis-undecaprenyl phosphate.UniRule annotation

Pathwayi

GO - Molecular functioni

  1. mannosyltransferase activity Source: InterPro
  2. transferase activity, transferring pentosyl groups Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. lipid A biosynthetic process Source: UniProtKB-HAMAP
  2. lipopolysaccharide biosynthetic process Source: UniProtKB-KW
  3. protein O-linked glycosylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism, Lipopolysaccharide biosynthesis

Enzyme and pathway databases

BioCyciESP399742:GJ0E-2121-MONOMER.
UniPathwayiUPA00037.

Protein family/group databases

CAZyiGT83. Glycosyltransferase Family 83.

Names & Taxonomyi

Protein namesi
Recommended name:
Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase (EC:2.4.2.43)
Alternative name(s):
4-amino-4-deoxy-L-arabinose lipid A transferase
Lipid IV(A) 4-amino-4-deoxy-L-arabinosyltransferase
Undecaprenyl phosphate-alpha-L-Ara4N transferase
Gene namesi
Name:arnT
Ordered Locus Names:Ent638_2075
OrganismiEnterobacter sp. (strain 638)
Taxonomic identifieri399742 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacter
ProteomesiUP000000230: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei8 – 2821Helical; Reviewed prediction
Add
BLAST
Transmembranei83 – 10321Helical; Reviewed prediction
Add
BLAST
Transmembranei111 – 13121Helical; Reviewed prediction
Add
BLAST
Transmembranei132 – 15221Helical; Reviewed prediction
Add
BLAST
Transmembranei176 – 19621Helical; Reviewed prediction
Add
BLAST
Transmembranei204 – 22421Helical; Reviewed prediction
Add
BLAST
Transmembranei255 – 27521Helical; Reviewed prediction
Add
BLAST
Transmembranei288 – 30821Helical; Reviewed prediction
Add
BLAST
Transmembranei317 – 33721Helical; Reviewed prediction
Add
BLAST
Transmembranei350 – 37021Helical; Reviewed prediction
Add
BLAST
Transmembranei380 – 40021Helical; Reviewed prediction
Add
BLAST
Transmembranei407 – 42721Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 553553Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferaseUniRule annotation
PRO_1000065665Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi399742.Ent638_2075.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1807.
HOGENOMiHOG000273002.
KOiK07264.
OMAiYLATEYR.
OrthoDBiEOG6NKQWN.

Family and domain databases

HAMAPiMF_01165. ArnT_transfer.
InterProiIPR022839. ArnT_tfrase.
IPR003342. Glyco_trans_39.
[Graphical view]
PfamiPF02366. PMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4WAM1-1 [UniParc]FASTAAdd to Basket

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MKSAQYSLVL LLGFTLLYLL PLEFRALWQP DETRYAEISR EMLSAGNWIV    50
PHFLDVRYFE KPVAGYWINN LSQMIFGHNN FSVRFGAVFS TTLSALMVAW 100
LAFRLWRDKT VAVLSGVIFL TCLLVYGVGT YAVLDPMITL WLVAAMCSFW 150
LGANAQTRAG KAGGYILLGL ACGMGVMTKG FLALAVPVLG VLPWVIAQKR 200
WKEVLLFGPL AIISATLITL PWALAIAKAE PTFWHYFFWV EHIQRFAEND 250
AQHKAPFWYY IPFLIAGCLP WVALLPGALK RSWNERHIES GTLYLLGWVV 300
MPLLFFSIAK GKLPTYILPC FAPLAILLAR HATQLVATPR TLKVNGWINT 350
VFGAVCALIV LLVLAPWGIA KHPIYASHEV LKVIQASIAF LVWALVGYLT 400
TRNNARLWQW AALCPLGIAL LVGGMIPDKV VYSKHPQAFV DLVRPELESS 450
RYILADSVGV GAGIAWEMKR SDITLYAKPG ELDYGLTTFA DAKDNFVSRD 500
DFASWLALHR KEGNVSLVKL LSKDSVLEDS DVPAPDKVYH KGRFLLFFYE 550
KTP 553
Length:553
Mass (Da):61,876
Last modified:May 29, 2007 - v1
Checksum:i7DE7980845DCE26C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000653 Genomic DNA. Translation: ABP60751.1.
RefSeqiWP_012017466.1. NC_009436.1.
YP_001176802.1. NC_009436.1.

Genome annotation databases

EnsemblBacteriaiABP60751; ABP60751; Ent638_2075.
GeneIDi5112196.
KEGGient:Ent638_2075.
PATRICi20413793. VBIEntSp101211_2226.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000653 Genomic DNA. Translation: ABP60751.1 .
RefSeqi WP_012017466.1. NC_009436.1.
YP_001176802.1. NC_009436.1.

3D structure databases

ModBasei Search...

Protein-protein interaction databases

STRINGi 399742.Ent638_2075.

Protein family/group databases

CAZyi GT83. Glycosyltransferase Family 83.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABP60751 ; ABP60751 ; Ent638_2075 .
GeneIDi 5112196.
KEGGi ent:Ent638_2075.
PATRICi 20413793. VBIEntSp101211_2226.

Phylogenomic databases

eggNOGi COG1807.
HOGENOMi HOG000273002.
KOi K07264.
OMAi YLATEYR.
OrthoDBi EOG6NKQWN.

Enzyme and pathway databases

UniPathwayi UPA00037 .
BioCyci ESP399742:GJ0E-2121-MONOMER.

Family and domain databases

HAMAPi MF_01165. ArnT_transfer.
InterProi IPR022839. ArnT_tfrase.
IPR003342. Glyco_trans_39.
[Graphical view ]
Pfami PF02366. PMT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the plant growth promoting endophytic bacterium Enterobacter sp. 638."
    Taghavi S., van der Lelie D., Hoffman A., Zhang Y.B., Walla M.D., Vangronsveld J., Newman L., Monchy S.
    PLoS Genet. 6:E1000943-E1000943(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 638.

Entry informationi

Entry nameiARNT_ENT38
AccessioniPrimary (citable) accession number: A4WAM1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: May 29, 2007
Last modified: September 3, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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