ID   MAO1_ENT38              Reviewed;         565 AA.
AC   A4WAJ3;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   29-MAY-2007, sequence version 1.
DT   16-JUN-2009, entry version 20.
DE   RecName: Full=NAD-dependent malic enzyme;
DE            Short=NAD-ME;
DE            EC=1.1.1.38;
GN   Name=sfcA; OrderedLocusNames=Ent638_2047;
OS   Enterobacter sp. (strain 638).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Enterobacter.
OX   NCBI_TaxID=399742;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T.,
RA   Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D.,
RA   Detter J.C., Han C., Tapia R., Gilna P., Schmutz J., Larimer F.,
RA   Land M., Hauser L., Kyrpides N., Kim E., Taghavi S., Newman L.,
RA   Vangronsveld J., van der Lelie D., Richardson P.;
RT   "Complete sequence of chromosome of Enterobacter sp. 638.";
RL   Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: (S)-malate + NAD(+) = pyruvate + CO(2) + NADH.
CC   -!- COFACTOR: Divalent metal cations. Prefers magnesium or manganese
CC       (By similarity).
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SIMILARITY: Belongs to the malic enzymes family.
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DR   EMBL; CP000653; ABP60723.1; -; Genomic_DNA.
DR   RefSeq; YP_001176774.1; -.
DR   GeneID; 5113463; -.
DR   GenomeReviews; CP000653_GR; Ent638_2047.
DR   KEGG; ent:Ent638_2047; -.
DR   NMPDR; fig|399742.4.peg.2000; -.
DR   OMA; A4WAJ3; IQDNNET.
DR   GO; GO:0016619; F:malate dehydrogenase (oxaloacetate-decarbox...; IEA:HAMAP.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0051287; F:NAD or NADH binding; IEA:InterPro.
DR   GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_01619; -; 1.
DR   InterPro; IPR015884; Malic_enzyme_CS.
DR   InterPro; IPR012301; Malic_N.
DR   InterPro; IPR012302; Malic_NAD_bd.
DR   InterPro; IPR001891; Malic_OxRdtase.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF00390; malic; 1.
DR   Pfam; PF03949; Malic_M; 1.
DR   PRINTS; PR00072; MALOXRDTASE.
DR   PROSITE; PS00331; MALIC_ENZYMES; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Metal-binding; NAD; Oxidoreductase.
FT   CHAIN         1    565       NAD-dependent malic enzyme.
FT                                /FTId=PRO_1000069531.
FT   ACT_SITE    104    104       Proton donor (By similarity).
FT   ACT_SITE    175    175       Proton acceptor (By similarity).
FT   METAL       246    246       Divalent metal cation (By similarity).
FT   METAL       247    247       Divalent metal cation (By similarity).
FT   METAL       270    270       Divalent metal cation (By similarity).
FT   BINDING     157    157       NAD (By similarity).
FT   BINDING     270    270       NAD (By similarity).
FT   BINDING     418    418       NAD (By similarity).
FT   SITE        270    270       Important for activity (By similarity).
SQ   SEQUENCE   565 AA;  63216 MW;  2E05AE43311C2F8A CRC64;
     MDKKTKKHRS LYIPYAGPVL LEFPLLNKGS AFSAEERSSF NLLGLLPEVV ETIEEQAERA
     WIQYQGFKTE IDKHIYLRNI QDTNETLFYR LVQNHLEEMM PVIYTPTVGA ACERFSEIYR
     RSRGVFISYE NRHNMDDILQ NVPNHNIKVI VVTDGERILG LGDQGIGGMG IPIGKLSLYT
     ACGGISPAYT LPVVLDVGTN NQQLLTDPLY MGWRHPRVTD DEYYQFVDDF IQAVKHRWPD
     VLLQFEDFAQ KNAMPLLNRY RDEICSFNDD IQGTAAVTAG TLIAASRAAG SQLSYQKIVF
     LGAGSAGCGI AEQIIAQTQR EGLSEELARS RVFMVDRFGL LTDAMPNLLP FQTKLVQKRE
     NLKNWDTDNE VLSLLDVVRN VKPDILIGVS GQTGLFTEEI IREMHKHCER PIVMPLSNPT
     SRVEATPQDI IVWTEGNALV ATGSPFDPVI WKDKVYPIAQ CNNSYIFPGI GLGVIASGAS
     RITDEMLMSA SETLAQHSPL VKNGEGLVLP ELKDIHIVSR AIAFAVGKMA QQQGVAVKTS
     ADALQQAIDE NFWMPEYRSY RRTSI
//