A4W9K3 (NADE_ENT38) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	Ent638_1704

Organism

Enterobacter sp. (strain 638) [Complete proteome] [HAMAP]

Taxonomic identifier

399742 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Enterobacter

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 275	275	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000077555
Regions
	Nucleotide binding	46 – 53	8	ATP By similarity
Sites
	Active site	48	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4W9K3 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: A6D28678C1AF4022	FASTA	275	30,434
	10 20 30 40 50 60 MALQQEIIQA LGVKPSIDAN AEIRRSIDFL KSYLKTNSFL KTLVLGISGG QDSTLAGKLC 70 80 90 100 110 120 QQAISELRQE TGDESLQFIA VRLPYGVQAD EQDCQDAIAF IHPDRVLTVN IKGSVLASEQ 130 140 150 160 170 180 ALREAGLELS DFVRGNEKAR ERMKAQYSIA GMTKGVVVGT DHAAEAVTGF FTKYGDGGTD 190 200 210 220 230 240 INPLFRLNKR QGKQLLAALG CPEHLYLKAP TADLEDDRPS LPDEAALGVT YENIDDYLEG 250 260 270 KTLDDTIART IEGWYLKTEH KRRPPITVFD DFWKR

References

[1]

"Complete sequence of chromosome of Enterobacter sp. 638." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Taghavi S., Newman L., Vangronsveld J., van der Lelie D., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 638.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000653 Genomic DNA. Translation: ABP60383.1.
RefSeq	YP_001176434.1. NC_009436.1.
3D structure databases
ProteinModelPortal	A4W9K3.
SMR	A4W9K3. Positions 3-275.
ModBase	Search...
Protein-protein interaction databases
STRING	A4W9K3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5112443.
GenomeReviews	Gene locus Ent638_1704 in contig CP000653_GR.
KEGG	ent:Ent638_1704.
NMPDR	fig|399742.4.peg.1680.
PATRIC	20413027. VBIEntSp101211_1845.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0171.
HOGENOM	HBG351567.
OMA	IAQYEIA.
PhylomeDB	A4W9K3.
ProtClustDB	PRK00768.
Enzyme and pathway databases
BioCyc	ESP42895:ENT638_1704-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_ENT38

Accession

Primary (citable) accession number: A4W9K3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 29, 2007
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program