A4W948 (GHRA_ENT38) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glyoxylate/hydroxypyruvate reductase A
EC=1.1.1.79
EC=1.1.1.81

Alternative name(s):
2-ketoacid reductase

Gene names

Name:	ghrA
Ordered Locus Names:	Ent638_1549

Organism

Enterobacter sp. (strain 638) [Complete proteome] [HAMAP]

Taxonomic identifier

399742 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Enterobacter

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively By similarity. HAMAP MF_01666
Catalytic activity	Glycolate + NADP+ = glyoxylate + NADPH. HAMAP MF_01666 D-glycerate + NAD(P)+ = hydroxypyruvate + NAD(P)H. HAMAP MF_01666
Subcellular location	Cytoplasm By similarity HAMAP MF_01666.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cofactor binding Inferred from electronic annotation. Source: InterPro glyoxylate reductase (NADP) activity Inferred from electronic annotation. Source: EC hydroxypyruvate reductase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	Glyoxylate/hydroxypyruvate reductase A HAMAP MF_01666		PRO_0000348363
Sites
	Active site	227	1	By similarity
	Active site	275	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4W948 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: F6733BFF34C5E533	FASTA	312	34,929
	10 20 30 40 50 60 MDIIFYHPTF DTPYWINALT AALPGARVRE WKRGDNEHAD YALVWHPPVE MLQGRDLKAV 70 80 90 100 110 120 FALGAGVDSI LSKLKAHPEM LPEHIPLFRL EDTGMGQQMQ EYAVSQVLHW FRRFDDYQAL 130 140 150 160 170 180 KQKSHWEPLA DYQREDFTIG ILGAGVLGSK VAEALAPWGF PLRCWSRSRK TYPGVQSFAG 190 200 210 220 230 240 ADELPAFLKG TRVLINLLPN TAETVGIINK GLLNQLADES YLMNLARGVH VIEEDLIDAL 250 260 270 280 290 300 NTGKLKGAML DVYSSEPLPV ESPLWAHPRV AMTPHIAAVT RPAEAVAYIA RTIEHLEQGK 310 AATRQVNRQL GY

References

[1]

"Complete sequence of chromosome of Enterobacter sp. 638." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Taghavi S., Newman L., Vangronsveld J., van der Lelie D., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 638.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000653 Genomic DNA. Translation: ABP60228.1.
RefSeq	YP_001176279.1. NC_009436.1.
3D structure databases
ProteinModelPortal	A4W948.
ModBase	Search...
Protein-protein interaction databases
STRING	A4W948.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5114519.
GenomeReviews	Gene locus Ent638_1549 in contig CP000653_GR.
KEGG	ent:Ent638_1549.
NMPDR	fig|399742.4.peg.1534.
PATRIC	20412713. VBIEntSp101211_1688.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0111.
HOGENOM	HBG731446.
OMA	EYVTHAV.
PhylomeDB	A4W948.
ProtClustDB	PRK15469.
Enzyme and pathway databases
BioCyc	ESP42895:ENT638_1549-MONOMER.
Family and domain databases
HAMAP	MF_01666. 2-Hacid_dh_C_GhrA. [Tree]
InterPro	IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR023514. GhrA. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K12972.
Pfam	PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. False negative. PS00670. D_2_HYDROXYACID_DH_2. False negative. PS00671. D_2_HYDROXYACID_DH_3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GHRA_ENT38

Accession

Primary (citable) accession number: A4W948

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	May 29, 2007
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program