Reviewed, UniProtKB/Swiss-Prot A4W8U9 (SYN_ENT38)

Names and origin

Protein names

Recommended name:
    Asparaginyl-tRNA synthetase
    EC=6.1.1.22
Alternative name(s):
    Asparagine--tRNA ligase
      Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	Ent638_1449

Organism

Enterobacter sp. (strain 638) [Complete proteome] [HAMAP]

Taxonomic identifier

399742 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Enterobacter

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00534
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP asparagine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 466	466	Asparaginyl-tRNA synthetase HAMAP MF_00534		PRO_1000061020

Sequences

	Sequence		Length	Mass (Da)
	A4W8U9-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 32EC2A0E8CFAE4B2	FASTA	466	52,071
	10 20 30 40 50 60 MSVVPVADVL QGRVAVDQEV TVRGWVRTRR DSKAGISFLA VYDGSCFDPV QAVINNSLPN 70 80 90 100 110 120 YNDDVLHLTT GCSVIVTGVV VASPGQGQSF ELQATAIEVT GWVEDPDTYP MAAKRHSIEY 130 140 150 160 170 180 LREVAHLRPR TNMIGAVARV RHTLAQALHR FFDEQGYFWV STPLITASDT EGAGEMFRVS 190 200 210 220 230 240 TLDLENLPRT PEGKVDFDKD FFGKEAFLTV SGQLNGETYA CALSKIYTFG PTFRAENSNT 250 260 270 280 290 300 SRHLAEFWML EPEVAFANLN DVAGLAEAML KYVFKAVLEE RADDMKFFAE RVDSDAVARL 310 320 330 340 350 360 ERFVSADFAQ VDYTDAVAIL EKCGEKFENP VYWGVDLSSE HERYLAEKHF KAPVVVKNYP 370 380 390 400 410 420 KDIKAFYMRL NEDGKTVAAM DVLAPGIGEI IGGSQREERL DVLDARMEEM GLNPADYSWY 430 440 450 460 RDLRRYGTVP HSGFGLGFER LIAYVTGVQN VRDVIAFPRT PRNASF

References

[1]

"Complete sequence of chromosome of Enterobacter sp. 638." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Taghavi S., Newman L., Vangronsveld J., van der Lelie D., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000653 Genomic DNA. Translation: ABP60129.1.
RefSeq	YP_001176180.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4W8U9.
Genome annotation databases
GeneID	5114414.
GenomeReviews	Gene locus Ent638_1449 in contig CP000653_GR.
KEGG	ent:Ent638_1449.
NMPDR	fig|399742.4.peg.1440.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	LQKKRHS.
Family and domain databases
HAMAP	MF_00534. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00457. asnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_ENT38

Accession

Primary (citable) accession number: A4W8U9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	May 29, 2007
Last modified:	November 3, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)