A4W8E5 (RLMF_ENT38) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase F
EC=2.1.1.181

Alternative name(s):
23S rRNA mA1618 methyltransferase
rRNA adenine N-6-methyltransferase

Gene names

Name:	rlmF
Ordered Locus Names:	Ent638_1294

Organism

Enterobacter sp. (strain 638) [Complete proteome] [HAMAP]

Taxonomic identifier

399742 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Enterobacter

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the adenine in position 1618 of 23S rRNA By similarity. HAMAP MF_01848
Catalytic activity	S-adenosyl-L-methionine + adenine(1618) in 23S rRNA = S-adenosyl-L-homocysteine + rRNA containing N(6)-methyladenine(1618) in 23S rRNA. HAMAP MF_01848
Subcellular location	Cytoplasm Potential HAMAP MF_01848.
Sequence similarities	Belongs to the methyltransferase superfamily. METTL16/rlmF family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA (adenine-N6-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	Ribosomal RNA large subunit methyltransferase F HAMAP MF_01848		PRO_0000349904

Sequences

	Sequence		Length	Mass (Da)
	A4W8E5 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 0E7ACE02CDFA22E8	FASTA	305	33,701
	10 20 30 40 50 60 MTAEKPGLHP RNRHRSRYDM NALCQSCPQL QAFIVQTPAG EPSVNFADPL AVKALNKALL 70 80 90 100 110 120 AHFYGVTQWD IPEGFLCPPV PGRADYVHHL ADLLAEDNAG TIPPQATVLD IGTGANLIYP 130 140 150 160 170 180 LIGVAEYGWR FTGSETGKDA YASAQAIVNG NEGLTRAIRL RRQKEASDIF NGIIHKNESY 190 200 210 220 230 240 DATMCNPPFH DSAETARAGS ERKRRNLGQA EDGALNFGGQ QHELWCEGGE VAFIEKMIAE 250 260 270 280 290 300 SKLFARQVKW FTTLVSRGDN LPPLYRALTA VGAVKVVKKE MAQGQKQSRF IAWTFMDDAK RRKSF

References

[1]

"Complete sequence of chromosome of Enterobacter sp. 638." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Taghavi S., Newman L., Vangronsveld J., van der Lelie D., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 638.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000653 Genomic DNA. Translation: ABP59975.1.
RefSeq	YP_001176026.1. NC_009436.1.
3D structure databases
ProteinModelPortal	A4W8E5.
ModBase	Search...
Protein-protein interaction databases
STRING	A4W8E5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5114257.
GenomeReviews	Gene locus Ent638_1294 in contig CP000653_GR.
KEGG	ent:Ent638_1294.
NMPDR	fig|399742.4.peg.1296.
PATRIC	20412190. VBIEntSp101211_1431.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3129.
HOGENOM	HBG747880.
OMA	DFANPEA.
PhylomeDB	A4W8E5.
ProtClustDB	PRK11727.
Enzyme and pathway databases
BioCyc	ESP42895:ENT638_1294-MONOMER.
Family and domain databases
HAMAP	MF_01848. 23SrRNA_methyltr_F. [Tree]
InterPro	IPR016909. rRNA_lsu_methyltransferase_F. IPR010286. SAM-Mtase_prd. [Graphical view]
KO	K06970.
PANTHER	PTHR13393. DUF890. 1 hit.
Pfam	PF05971. Methyltransf_10. 1 hit. [Graphical view]
PIRSF	PIRSF029038. Mtase_YbiN_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMF_ENT38

Accession

Primary (citable) accession number: A4W8E5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	May 29, 2007
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program