Reviewed, UniProtKB/Swiss-Prot A4W846 (SYQ_ENT38)

Names and origin

Protein names

Recommended name:
    Glutaminyl-tRNA synthetase
    EC=6.1.1.18
Alternative name(s):
    Glutamine--tRNA ligase
      Short name=GlnRS

Gene names

Name:	glnS
Ordered Locus Names:	Ent638_1195

Organism

Enterobacter sp. (strain 638) [Complete proteome] [HAMAP]

Taxonomic identifier

399742 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Enterobacter

Protein attributes

Sequence length	555 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP MF_00126
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00126
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 555	555	Glutaminyl-tRNA synthetase HAMAP MF_00126		PRO_1000057820
Regions
	Motif	34 – 44	11	"HIGH" region HAMAP MF_00126
	Motif	268 – 272	5	"KMSKS" region HAMAP MF_00126
Sites
	Binding site	271	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4W846-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: CA5CC100560616A2	FASTA	555	63,506
	10 20 30 40 50 60 MSEAEARPSN FIRQIIDEDL ASGKHTTVHT RFPPEPNGYL HIGHAKSICL NFGIAQDYQG 70 80 90 100 110 120 QCNLRFDDTN PVKEDIEYVE SIKNDVQWLG FNWSGDICYS SDYFDQLFAY AVELINKGLA 130 140 150 160 170 180 YVDELSADEI REYRGSLTAP GKNSPYRDRS VEENLALFEK MRAGGFEEGK ACLRAKIDMA 190 200 210 220 230 240 SPFIVMRDPV LYRIKFAEHH QTGNKWCIYP MYDFTHCISD ALEGITHSLC TLEFQDNRRL 250 260 270 280 290 300 YDWVLDNITI PVHPRQYEFS RLNLEYTVMS KRKLNLLVTD KHVEGWDDPR MPTISGLRRR 310 320 330 340 350 360 GYSASSIREF IKRIGVTKQD NTIEMASLES CIREDLNENA PRAMAVIDPV KLVIENYPQG 370 380 390 400 410 420 ESELVTMPNH PSKPEMGSRE VPFSAEIWID RADFREEANK QYKRLVMGKE VRLRNAYVIK 430 440 450 460 470 480 AERVEKDTEG NITTIFCSYD AETLSKDPAD GRKVKGVIHW VSAAHALPVE IRLYDRLFSV 490 500 510 520 530 540 PNPGAAEDFL ATINPESLLI KQGYAEPSLK NAETGKAYQF EREGYFCLDS RYATATKLVF 550 NRTVGLRDTW AKAGE

References

[1]

"Complete sequence of chromosome of Enterobacter sp. 638." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Taghavi S., Newman L., Vangronsveld J., van der Lelie D., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000653 Genomic DNA. Translation: ABP59876.1.
RefSeq	YP_001175927.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4W846.
Genome annotation databases
GeneID	5114152.
GenomeReviews	Gene locus Ent638_1195 in contig CP000653_GR.
KEGG	ent:Ent638_1195.
NMPDR	fig|399742.4.peg.1202.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	RMPTIAG.
Family and domain databases
HAMAP	MF_00126. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth_Ic. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ic_codon-bd. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. [Graphical view]
Gene3D	G3DSA:2.40.240.10. Rbsml_L25/Gln-tRNA_synth_b-brl. 1 hit.
PANTHER	PTHR10119:SF3. GlnS. 1 hit. PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00440. glnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYQ_ENT38

Accession

Primary (citable) accession number: A4W846

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	May 29, 2007
Last modified:	November 3, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)