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A4W827 (SYL_ENT38) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Ent638_1176
OrganismEnterobacter sp. (strain 638) [Complete proteome] [HAMAP]
Taxonomic identifier399742 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacter

Protein attributes

Sequence length894 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 894894Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000057344

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif653 – 6575"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6561ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A4W827 [UniParc].

Last modified May 29, 2007. Version 1.
Checksum: 96A990F72D243966

FASTA894100,942
        10         20         30         40         50         60 
MQEQYRPEEI ETKVQQHWDE KRTFEVTEDE SKEKYYCLSM LPYPSGRLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VIARYQRMLG KNVLQPIGWD AFGLPAEGAA VKNNTAPAPW TYDNIAYMKN QLKMLGFGYD 

       130        140        150        160        170        180 
WSRELATCTP EYYRWEQKFF TELYKKGLVY KKTSAVNWCP NDQTVLANEQ VIDGCCWRCD 

       190        200        210        220        230        240 
TKVERKEIPQ WFIKITAYAD ELLRDLDNLD HWPDTVKTMQ RNWIGRSEGV EITFNVENSD 

       250        260        270        280        290        300 
QKLTVYTTRP DTFMGATYLA VAAGHPLAQQ AAANNPALAA FIDECRNTKV AEADMATMEK 

       310        320        330        340        350        360 
KGVATGFNAI HPLTGEAVPV WAANFVLMEY GTGAVMAVPG HDQRDYEFAT KYGLPIKRVI 

       370        380        390        400        410        420 
KSPEQKAYEH ALEYGLGSAK NPNDFEPLTH NQRSEFLGLL TAELNKPYLE KDGETYNSGK 

       430        440        450        460        470        480 
FDGLSFEAAF NSIAAALITK GVGERKVNYR LRDWGVSRQR YWGAPIPMVT LEDGTVLPTP 

       490        500        510        520        530        540 
EDQLPVILPE DVVMDGISSP IKADPEWAKT TVNGQPALRE TDTFDTFMES SWYYARYTCP 

       550        560        570        580        590        600 
QYQEGMLDSK AANYWLPVDI YIGGIEHAIM HLLYFRFFHK LMRDAGLVNS DEPAKQLLCQ 

       610        620        630        640        650        660 
GMVLADAFYY VGVNGERNWV SPVDAIVERD EKGRIVKAKD AAGNELVYTG MSKMSKSKNN 

       670        680        690        700        710        720 
GIDPQVMVER YGADTVRLFM MFASPADMTL EWQESGVEGA NRFLKRVWKL VYEHTSQGEA 

       730        740        750        760        770        780 
PALNVGALSE DQQALRRDVH KTIAKVTDDI GRRQTFNTAI AAIMELMNKL ARAPQEGEQD 

       790        800        810        820        830        840 
RALMREALLA VVRMLNPFTP HACFTLWQEL KGEGDIDNAP WPVADESAMV ENTTLVVVQV 

       850        860        870        880        890 
NGKVRGKITV AVDATEEQVR ERAGQEHLVA KYLEGVTVRK VIYVPGKLLN LVVG 

« Hide

References

[1]"Genome sequence of the plant growth promoting endophytic bacterium Enterobacter sp. 638."
Taghavi S., van der Lelie D., Hoffman A., Zhang Y.B., Walla M.D., Vangronsveld J., Newman L., Monchy S.
PLoS Genet. 6:E1000943-E1000943(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 638.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000653 Genomic DNA. Translation: ABP59857.1.
RefSeqYP_001175908.1. NC_009436.1.

3D structure databases

ProteinModelPortalA4W827.
SMRA4W827. Positions 228-447.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING399742.Ent638_1176.

Proteomic databases

PRIDEA4W827.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABP59857; ABP59857; Ent638_1176.
GeneID5114125.
KEGGent:Ent638_1176.
PATRIC20411928. VBIEntSp101211_1312.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycESP399742:GJ0E-1204-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 2 hits.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsTIGR00396. leuS_bact. 2 hits.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_ENT38
AccessionPrimary (citable) accession number: A4W827
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: May 29, 2007
Last modified: April 16, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries