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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Enterobacter sp. (strain 638)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei656 – 6561ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciESP399742:GJ0E-1204-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Ent638_1176
OrganismiEnterobacter sp. (strain 638)
Taxonomic identifieri399742 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacter
ProteomesiUP000000230 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 894894Leucine--tRNA ligasePRO_1000057344Add
BLAST

Proteomic databases

PRIDEiA4W827.

Interactioni

Protein-protein interaction databases

STRINGi399742.Ent638_1176.

Structurei

3D structure databases

ProteinModelPortaliA4W827.
SMRiA4W827. Positions 228-447.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi653 – 6575"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 2 hits.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00396. leuS_bact. 2 hits.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4W827-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQEQYRPEEI ETKVQQHWDE KRTFEVTEDE SKEKYYCLSM LPYPSGRLHM
60 70 80 90 100
GHVRNYTIGD VIARYQRMLG KNVLQPIGWD AFGLPAEGAA VKNNTAPAPW
110 120 130 140 150
TYDNIAYMKN QLKMLGFGYD WSRELATCTP EYYRWEQKFF TELYKKGLVY
160 170 180 190 200
KKTSAVNWCP NDQTVLANEQ VIDGCCWRCD TKVERKEIPQ WFIKITAYAD
210 220 230 240 250
ELLRDLDNLD HWPDTVKTMQ RNWIGRSEGV EITFNVENSD QKLTVYTTRP
260 270 280 290 300
DTFMGATYLA VAAGHPLAQQ AAANNPALAA FIDECRNTKV AEADMATMEK
310 320 330 340 350
KGVATGFNAI HPLTGEAVPV WAANFVLMEY GTGAVMAVPG HDQRDYEFAT
360 370 380 390 400
KYGLPIKRVI KSPEQKAYEH ALEYGLGSAK NPNDFEPLTH NQRSEFLGLL
410 420 430 440 450
TAELNKPYLE KDGETYNSGK FDGLSFEAAF NSIAAALITK GVGERKVNYR
460 470 480 490 500
LRDWGVSRQR YWGAPIPMVT LEDGTVLPTP EDQLPVILPE DVVMDGISSP
510 520 530 540 550
IKADPEWAKT TVNGQPALRE TDTFDTFMES SWYYARYTCP QYQEGMLDSK
560 570 580 590 600
AANYWLPVDI YIGGIEHAIM HLLYFRFFHK LMRDAGLVNS DEPAKQLLCQ
610 620 630 640 650
GMVLADAFYY VGVNGERNWV SPVDAIVERD EKGRIVKAKD AAGNELVYTG
660 670 680 690 700
MSKMSKSKNN GIDPQVMVER YGADTVRLFM MFASPADMTL EWQESGVEGA
710 720 730 740 750
NRFLKRVWKL VYEHTSQGEA PALNVGALSE DQQALRRDVH KTIAKVTDDI
760 770 780 790 800
GRRQTFNTAI AAIMELMNKL ARAPQEGEQD RALMREALLA VVRMLNPFTP
810 820 830 840 850
HACFTLWQEL KGEGDIDNAP WPVADESAMV ENTTLVVVQV NGKVRGKITV
860 870 880 890
AVDATEEQVR ERAGQEHLVA KYLEGVTVRK VIYVPGKLLN LVVG
Length:894
Mass (Da):100,942
Last modified:May 29, 2007 - v1
Checksum:i96A990F72D243966
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000653 Genomic DNA. Translation: ABP59857.1.
RefSeqiWP_012016576.1. NC_009436.1.
YP_001175908.1. NC_009436.1.

Genome annotation databases

EnsemblBacteriaiABP59857; ABP59857; Ent638_1176.
KEGGient:Ent638_1176.
PATRICi20411928. VBIEntSp101211_1312.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000653 Genomic DNA. Translation: ABP59857.1.
RefSeqiWP_012016576.1. NC_009436.1.
YP_001175908.1. NC_009436.1.

3D structure databases

ProteinModelPortaliA4W827.
SMRiA4W827. Positions 228-447.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi399742.Ent638_1176.

Proteomic databases

PRIDEiA4W827.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABP59857; ABP59857; Ent638_1176.
KEGGient:Ent638_1176.
PATRICi20411928. VBIEntSp101211_1312.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciESP399742:GJ0E-1204-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 2 hits.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00396. leuS_bact. 2 hits.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the plant growth promoting endophytic bacterium Enterobacter sp. 638."
    Taghavi S., van der Lelie D., Hoffman A., Zhang Y.B., Walla M.D., Vangronsveld J., Newman L., Monchy S.
    PLoS Genet. 6:E1000943-E1000943(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 638.

Entry informationi

Entry nameiSYL_ENT38
AccessioniPrimary (citable) accession number: A4W827
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: May 29, 2007
Last modified: April 1, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.