Reviewed, UniProtKB/Swiss-Prot A4W7Z3 (MTNC_ENT38)

Names and origin

Protein names

Recommended name:
    Enolase-phosphatase E1
    EC=3.1.3.77
Alternative name(s):
    2,3-diketo-5-methylthio-1-phosphopentane phosphatase

Gene names

Name:	mtnC
Ordered Locus Names:	Ent638_1142

Organism

Enterobacter sp. (strain 638) [Complete proteome] [HAMAP]

Taxonomic identifier

399742 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Enterobacter

Protein attributes

Sequence length	229 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) By similarity. HAMAP MF_01681
Catalytic activity	5-(methylthio)-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + phosphate. HAMAP MF_01681
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01681
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6. HAMAP MF_01681 Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 4/6.
Subunit structure	Monomer By similarity. HAMAP MF_01681
Sequence similarities	Belongs to the HAD-like hydrolase superfamily. MasA/mtnC family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-methionine biosynthetic process from S-adenosylmethionine Inferred from electronic annotation. Source: HAMAP L-methionine salvage from methylthioadenosine Inferred from electronic annotation. Source: InterPro
Molecular function	2,3-diketo-5-methylthiopentyl-1-phosphate enolase activity Inferred from electronic annotation. Source: HAMAP 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase activity Inferred from electronic annotation. Source: HAMAP acireductone synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 229	229	Enolase-phosphatase E1 HAMAP MF_01681		PRO_0000357363

Sequences

	Sequence		Length	Mass (Da)
	A4W7Z3-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 8A478773CE5DB3BF	FASTA	229	25,503
	10 20 30 40 50 60 MIRAIVTDIE GTTSDIRFVH NVLFPYARER LAAFVTAQQY AEPVKSILDN LRDEIAAPHA 70 80 90 100 110 120 TIGELVDALF TFMDEDRKST ALKALQGIIW QDGYVNGDFT GQLYPDVLPA LEKWKAQGID 130 140 150 160 170 180 LYVYSSGSVA AQKLLFGYSD EGDITHLFSG YFDTRVGAKR DVQSYQNIAA QIGVSPSQIL 190 200 210 220 FLSDIHEELD AAEQAGFRTL QLIRGDDDGA SHHHQVHQFD EINPEQIPS

References

[1]

"Complete sequence of chromosome of Enterobacter sp. 638." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Taghavi S., Newman L., Vangronsveld J., van der Lelie D., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000653 Genomic DNA. Translation: ABP59823.1.
RefSeq	YP_001175874.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4W7Z3.
Genome annotation databases
GeneID	5114091.
GenomeReviews	Gene locus Ent638_1142 in contig CP000653_GR.
KEGG	ent:Ent638_1142.
NMPDR	fig|399742.4.peg.1149.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG4229.
HOGENOM	HBG294242.
OMA	TTDLNFI.
PhylomeDB	A4W7Z3.
Family and domain databases
HAMAP	MF_01681. Salvage_MtnC. [Tree]
InterPro	IPR005834. Dehalogen-like_hydro. IPR010041. Enolase_ppase. IPR005833. Haloacid_DH/epoxide_hydro. [Graphical view]
PANTHER	PTHR20371. Enolase_ppase. 1 hit.
Pfam	PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00413. HADHALOGNASE.
TIGRFAMs	TIGR01691. enolase-ppase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTNC_ENT38

Accession

Primary (citable) accession number: A4W7Z3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	May 29, 2007
Last modified:	February 9, 2010
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)