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A4W7D2

- BGAL_ENT38

UniProt

A4W7D2 - BGAL_ENT38

Protein

Beta-galactosidase

Gene

lacZ

Organism
Enterobacter sp. (strain 638)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 1 (29 May 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

    Cofactori

    Binds 2 magnesium ions per monomer.UniRule annotation
    Binds 1 sodium ion per monomer.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei104 – 1041SubstrateUniRule annotation
    Metal bindingi203 – 2031SodiumUniRule annotation
    Binding sitei203 – 2031SubstrateUniRule annotation
    Sitei359 – 3591Transition state stabilizerUniRule annotation
    Sitei393 – 3931Transition state stabilizerUniRule annotation
    Metal bindingi418 – 4181Magnesium 1UniRule annotation
    Metal bindingi420 – 4201Magnesium 1UniRule annotation
    Active sitei463 – 4631Proton donorUniRule annotation
    Metal bindingi463 – 4631Magnesium 1UniRule annotation
    Binding sitei463 – 4631SubstrateUniRule annotation
    Active sitei539 – 5391NucleophileUniRule annotation
    Metal bindingi599 – 5991Magnesium 2UniRule annotation
    Metal bindingi603 – 6031Sodium; via carbonyl oxygenUniRule annotation
    Metal bindingi606 – 6061SodiumUniRule annotation
    Binding sitei606 – 6061SubstrateUniRule annotation
    Binding sitei1004 – 10041SubstrateUniRule annotation

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. carbohydrate binding Source: InterPro
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Sodium

    Enzyme and pathway databases

    BioCyciESP399742:GJ0E-955-MONOMER.

    Protein family/group databases

    CAZyiGH2. Glycoside Hydrolase Family 2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
    Short name:
    Beta-galUniRule annotation
    Alternative name(s):
    LactaseUniRule annotation
    Gene namesi
    Name:lacZUniRule annotation
    Ordered Locus Names:Ent638_0928
    OrganismiEnterobacter sp. (strain 638)
    Taxonomic identifieri399742 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacter
    ProteomesiUP000000230: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10281028Beta-galactosidasePRO_0000366985Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi399742.Ent638_0928.

    Structurei

    3D structure databases

    ProteinModelPortaliA4W7D2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni539 – 5424Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 2 family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG3250.
    HOGENOMiHOG000252443.
    KOiK01190.
    OMAiDPKKPYI.
    OrthoDBiEOG6XWV0T.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPiMF_01687. Beta_gal.
    InterProiIPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view]
    PRINTSiPR00132. GLHYDRLASE2.
    SMARTiSM01038. Bgal_small_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A4W7D2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFTASPMSLS KILARRDWEN PGVTQWHRLP AHAPFNSWRD EASARADDNA     50
    SRKRSLNGDW QFSYYAAPEQ VPDSWVTEDC ADAVTTPVPS NWQMQGFDTP 100
    IYTNVTYPIP VNPPFVPAEN PTGCYSLTFE VDEQWLESGQ TRIVFDGVNS 150
    AFYLWCNGKW MGYSQDSRLP AEFDLSAVLR PGTNRLAVLV LRWCDGSYLE 200
    DQDMWRMSGI FRDVSLLHKP HTHIADYHAV TELNADYDRA KLQVEVALAG 250
    EQFADCEVTV TLWRDGLSVA TASAKPGSAI IDERGNWAER LNVTLPVNDP 300
    ALWSAETPEL YRLTFALRDG QGEILDVEAC DVGFRCVEIS NGLLKVNGKP 350
    LLIRGVNRHE HHPENGQVMD EATMRRDIEL MKQHNFNAVR CSHYPNHPLW 400
    YTLCDRYGLY VVDEANIETH GMVPMSRLAD DPRWLPAMSE RVTRMVLRDR 450
    NHPSIIIWSL GNESGHGANH DALYRWVKTT DPTRPVQYEG GGANTAATDI 500
    VCPMYARVDQ DQPFEAVPKW SLKKWIGMPD ETRPLILCEY AHAMGNSFGG 550
    FAKYWQAFRN HPRLQGGFVW DWVDQALTKK DDNGNAFWAY GGDFGDTPND 600
    RQFCLNGLVF PDRTPHPALF EAQRAQQFFN FTLVSTSPLV IDVHSDYLFR 650
    QCDNEQLRWN IARDGEVLAS GEVALTIAPQ QTQRIEIDAP EFAAAAGEIW 700
    LNVDIVQTAA TAWSPADHRC AWDQWQLPAP LYIAPPVEGT AKPDLKVKED 750
    VLEVSHQSQR WHFDRASGNL TQWWNNGTAT LLAPLNDNFT RAPLDNDIGV 800
    SEATRIDPNA WVERWKAAGM YNLTPRLLLC EGEQLAQAVT ITTLHAWESN 850
    GKALFLSRKV WKIDRAGVLH GDVQVQVAND IPQPARIGLS CQLAQTPQTA 900
    SWLGLGPDEN YPDRKLAARQ GRWTLPLDAL HTAYIFPTDN GLRCDTRELT 950
    FDTHQLQGDF HFSLSRYSQQ QLRDTSHHHL LEAEPGCWLN IDAFHMGVGG 1000
    DDSWSPSVSP EFILQRREMR YAFSWRQD 1028
    Length:1,028
    Mass (Da):116,441
    Last modified:May 29, 2007 - v1
    Checksum:i57258FFE65A78032
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000653 Genomic DNA. Translation: ABP59612.1.
    RefSeqiWP_012016333.1. NC_009436.1.
    YP_001175663.1. NC_009436.1.

    Genome annotation databases

    EnsemblBacteriaiABP59612; ABP59612; Ent638_0928.
    GeneIDi5110958.
    KEGGient:Ent638_0928.
    PATRICi20411432. VBIEntSp101211_1065.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000653 Genomic DNA. Translation: ABP59612.1 .
    RefSeqi WP_012016333.1. NC_009436.1.
    YP_001175663.1. NC_009436.1.

    3D structure databases

    ProteinModelPortali A4W7D2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 399742.Ent638_0928.

    Protein family/group databases

    CAZyi GH2. Glycoside Hydrolase Family 2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABP59612 ; ABP59612 ; Ent638_0928 .
    GeneIDi 5110958.
    KEGGi ent:Ent638_0928.
    PATRICi 20411432. VBIEntSp101211_1065.

    Phylogenomic databases

    eggNOGi COG3250.
    HOGENOMi HOG000252443.
    KOi K01190.
    OMAi DPKKPYI.
    OrthoDBi EOG6XWV0T.

    Enzyme and pathway databases

    BioCyci ESP399742:GJ0E-955-MONOMER.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPi MF_01687. Beta_gal.
    InterProi IPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00132. GLHYDRLASE2.
    SMARTi SM01038. Bgal_small_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the plant growth promoting endophytic bacterium Enterobacter sp. 638."
      Taghavi S., van der Lelie D., Hoffman A., Zhang Y.B., Walla M.D., Vangronsveld J., Newman L., Monchy S.
      PLoS Genet. 6:E1000943-E1000943(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 638.

    Entry informationi

    Entry nameiBGAL_ENT38
    AccessioniPrimary (citable) accession number: A4W7D2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: May 29, 2007
    Last modified: October 1, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3