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A4W7D2

- BGAL_ENT38

UniProt

A4W7D2 - BGAL_ENT38

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Protein

Beta-galactosidase

Gene

lacZ

Organism
Enterobacter sp. (strain 638)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Binds 2 magnesium ions per monomer.UniRule annotation
Binds 1 sodium ion per monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei104 – 1041SubstrateUniRule annotation
Metal bindingi203 – 2031SodiumUniRule annotation
Binding sitei203 – 2031SubstrateUniRule annotation
Sitei359 – 3591Transition state stabilizerUniRule annotation
Sitei393 – 3931Transition state stabilizerUniRule annotation
Metal bindingi418 – 4181Magnesium 1UniRule annotation
Metal bindingi420 – 4201Magnesium 1UniRule annotation
Active sitei463 – 4631Proton donorUniRule annotation
Metal bindingi463 – 4631Magnesium 1UniRule annotation
Binding sitei463 – 4631SubstrateUniRule annotation
Active sitei539 – 5391NucleophileUniRule annotation
Metal bindingi599 – 5991Magnesium 2UniRule annotation
Metal bindingi603 – 6031Sodium; via carbonyl oxygenUniRule annotation
Metal bindingi606 – 6061SodiumUniRule annotation
Binding sitei606 – 6061SubstrateUniRule annotation
Binding sitei1004 – 10041SubstrateUniRule annotation

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Enzyme and pathway databases

BioCyciESP399742:GJ0E-955-MONOMER.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
Short name:
Beta-galUniRule annotation
Alternative name(s):
LactaseUniRule annotation
Gene namesi
Name:lacZUniRule annotation
Ordered Locus Names:Ent638_0928
OrganismiEnterobacter sp. (strain 638)
Taxonomic identifieri399742 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacter
ProteomesiUP000000230: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10281028Beta-galactosidasePRO_0000366985Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi399742.Ent638_0928.

Structurei

3D structure databases

ProteinModelPortaliA4W7D2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni539 – 5424Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000252443.
KOiK01190.
OMAiDPKKPYI.
OrthoDBiEOG6XWV0T.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4W7D2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFTASPMSLS KILARRDWEN PGVTQWHRLP AHAPFNSWRD EASARADDNA
60 70 80 90 100
SRKRSLNGDW QFSYYAAPEQ VPDSWVTEDC ADAVTTPVPS NWQMQGFDTP
110 120 130 140 150
IYTNVTYPIP VNPPFVPAEN PTGCYSLTFE VDEQWLESGQ TRIVFDGVNS
160 170 180 190 200
AFYLWCNGKW MGYSQDSRLP AEFDLSAVLR PGTNRLAVLV LRWCDGSYLE
210 220 230 240 250
DQDMWRMSGI FRDVSLLHKP HTHIADYHAV TELNADYDRA KLQVEVALAG
260 270 280 290 300
EQFADCEVTV TLWRDGLSVA TASAKPGSAI IDERGNWAER LNVTLPVNDP
310 320 330 340 350
ALWSAETPEL YRLTFALRDG QGEILDVEAC DVGFRCVEIS NGLLKVNGKP
360 370 380 390 400
LLIRGVNRHE HHPENGQVMD EATMRRDIEL MKQHNFNAVR CSHYPNHPLW
410 420 430 440 450
YTLCDRYGLY VVDEANIETH GMVPMSRLAD DPRWLPAMSE RVTRMVLRDR
460 470 480 490 500
NHPSIIIWSL GNESGHGANH DALYRWVKTT DPTRPVQYEG GGANTAATDI
510 520 530 540 550
VCPMYARVDQ DQPFEAVPKW SLKKWIGMPD ETRPLILCEY AHAMGNSFGG
560 570 580 590 600
FAKYWQAFRN HPRLQGGFVW DWVDQALTKK DDNGNAFWAY GGDFGDTPND
610 620 630 640 650
RQFCLNGLVF PDRTPHPALF EAQRAQQFFN FTLVSTSPLV IDVHSDYLFR
660 670 680 690 700
QCDNEQLRWN IARDGEVLAS GEVALTIAPQ QTQRIEIDAP EFAAAAGEIW
710 720 730 740 750
LNVDIVQTAA TAWSPADHRC AWDQWQLPAP LYIAPPVEGT AKPDLKVKED
760 770 780 790 800
VLEVSHQSQR WHFDRASGNL TQWWNNGTAT LLAPLNDNFT RAPLDNDIGV
810 820 830 840 850
SEATRIDPNA WVERWKAAGM YNLTPRLLLC EGEQLAQAVT ITTLHAWESN
860 870 880 890 900
GKALFLSRKV WKIDRAGVLH GDVQVQVAND IPQPARIGLS CQLAQTPQTA
910 920 930 940 950
SWLGLGPDEN YPDRKLAARQ GRWTLPLDAL HTAYIFPTDN GLRCDTRELT
960 970 980 990 1000
FDTHQLQGDF HFSLSRYSQQ QLRDTSHHHL LEAEPGCWLN IDAFHMGVGG
1010 1020
DDSWSPSVSP EFILQRREMR YAFSWRQD
Length:1,028
Mass (Da):116,441
Last modified:May 29, 2007 - v1
Checksum:i57258FFE65A78032
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000653 Genomic DNA. Translation: ABP59612.1.
RefSeqiWP_012016333.1. NC_009436.1.
YP_001175663.1. NC_009436.1.

Genome annotation databases

EnsemblBacteriaiABP59612; ABP59612; Ent638_0928.
GeneIDi5110958.
KEGGient:Ent638_0928.
PATRICi20411432. VBIEntSp101211_1065.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000653 Genomic DNA. Translation: ABP59612.1 .
RefSeqi WP_012016333.1. NC_009436.1.
YP_001175663.1. NC_009436.1.

3D structure databases

ProteinModelPortali A4W7D2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 399742.Ent638_0928.

Protein family/group databases

CAZyi GH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABP59612 ; ABP59612 ; Ent638_0928 .
GeneIDi 5110958.
KEGGi ent:Ent638_0928.
PATRICi 20411432. VBIEntSp101211_1065.

Phylogenomic databases

eggNOGi COG3250.
HOGENOMi HOG000252443.
KOi K01190.
OMAi DPKKPYI.
OrthoDBi EOG6XWV0T.

Enzyme and pathway databases

BioCyci ESP399742:GJ0E-955-MONOMER.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_01687. Beta_gal.
InterProi IPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SMARTi SM01038. Bgal_small_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the plant growth promoting endophytic bacterium Enterobacter sp. 638."
    Taghavi S., van der Lelie D., Hoffman A., Zhang Y.B., Walla M.D., Vangronsveld J., Newman L., Monchy S.
    PLoS Genet. 6:E1000943-E1000943(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 638.

Entry informationi

Entry nameiBGAL_ENT38
AccessioniPrimary (citable) accession number: A4W7D2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 29, 2007
Last modified: October 1, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3