Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Beta-galactosidase

Gene

lacZ

Organism
Enterobacter sp. (strain 638)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 2 magnesium ions per monomer.UniRule annotation
  • Na+UniRule annotationNote: Binds 1 sodium ion per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei104SubstrateUniRule annotation1
Metal bindingi203SodiumUniRule annotation1
Binding sitei203SubstrateUniRule annotation1
Sitei359Transition state stabilizerUniRule annotation1
Sitei393Transition state stabilizerUniRule annotation1
Metal bindingi418Magnesium 1UniRule annotation1
Metal bindingi420Magnesium 1UniRule annotation1
Active sitei463Proton donorUniRule annotation1
Metal bindingi463Magnesium 1UniRule annotation1
Binding sitei463SubstrateUniRule annotation1
Active sitei539NucleophileUniRule annotation1
Metal bindingi599Magnesium 2UniRule annotation1
Metal bindingi603Sodium; via carbonyl oxygenUniRule annotation1
Metal bindingi606SodiumUniRule annotation1
Binding sitei606SubstrateUniRule annotation1
Binding sitei1004SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
Short name:
Beta-galUniRule annotation
Alternative name(s):
LactaseUniRule annotation
Gene namesi
Name:lacZUniRule annotation
Ordered Locus Names:Ent638_0928
OrganismiEnterobacter sp. (strain 638)
Taxonomic identifieri399742 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEnterobacter
Proteomesi
  • UP000000230 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003669851 – 1028Beta-galactosidaseAdd BLAST1028

Proteomic databases

PRIDEiA4W7D2.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi399742.Ent638_0928.

Structurei

3D structure databases

ProteinModelPortaliA4W7D2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni539 – 542Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CNT. Bacteria.
COG3250. LUCA.
HOGENOMiHOG000252443.
KOiK01190.
OMAiGMVPMSR.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. GH-type_carb-bd.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006103. Glyco_hydro_2_cat.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR032312. LacZ_4.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF16353. DUF4981. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4W7D2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFTASPMSLS KILARRDWEN PGVTQWHRLP AHAPFNSWRD EASARADDNA
60 70 80 90 100
SRKRSLNGDW QFSYYAAPEQ VPDSWVTEDC ADAVTTPVPS NWQMQGFDTP
110 120 130 140 150
IYTNVTYPIP VNPPFVPAEN PTGCYSLTFE VDEQWLESGQ TRIVFDGVNS
160 170 180 190 200
AFYLWCNGKW MGYSQDSRLP AEFDLSAVLR PGTNRLAVLV LRWCDGSYLE
210 220 230 240 250
DQDMWRMSGI FRDVSLLHKP HTHIADYHAV TELNADYDRA KLQVEVALAG
260 270 280 290 300
EQFADCEVTV TLWRDGLSVA TASAKPGSAI IDERGNWAER LNVTLPVNDP
310 320 330 340 350
ALWSAETPEL YRLTFALRDG QGEILDVEAC DVGFRCVEIS NGLLKVNGKP
360 370 380 390 400
LLIRGVNRHE HHPENGQVMD EATMRRDIEL MKQHNFNAVR CSHYPNHPLW
410 420 430 440 450
YTLCDRYGLY VVDEANIETH GMVPMSRLAD DPRWLPAMSE RVTRMVLRDR
460 470 480 490 500
NHPSIIIWSL GNESGHGANH DALYRWVKTT DPTRPVQYEG GGANTAATDI
510 520 530 540 550
VCPMYARVDQ DQPFEAVPKW SLKKWIGMPD ETRPLILCEY AHAMGNSFGG
560 570 580 590 600
FAKYWQAFRN HPRLQGGFVW DWVDQALTKK DDNGNAFWAY GGDFGDTPND
610 620 630 640 650
RQFCLNGLVF PDRTPHPALF EAQRAQQFFN FTLVSTSPLV IDVHSDYLFR
660 670 680 690 700
QCDNEQLRWN IARDGEVLAS GEVALTIAPQ QTQRIEIDAP EFAAAAGEIW
710 720 730 740 750
LNVDIVQTAA TAWSPADHRC AWDQWQLPAP LYIAPPVEGT AKPDLKVKED
760 770 780 790 800
VLEVSHQSQR WHFDRASGNL TQWWNNGTAT LLAPLNDNFT RAPLDNDIGV
810 820 830 840 850
SEATRIDPNA WVERWKAAGM YNLTPRLLLC EGEQLAQAVT ITTLHAWESN
860 870 880 890 900
GKALFLSRKV WKIDRAGVLH GDVQVQVAND IPQPARIGLS CQLAQTPQTA
910 920 930 940 950
SWLGLGPDEN YPDRKLAARQ GRWTLPLDAL HTAYIFPTDN GLRCDTRELT
960 970 980 990 1000
FDTHQLQGDF HFSLSRYSQQ QLRDTSHHHL LEAEPGCWLN IDAFHMGVGG
1010 1020
DDSWSPSVSP EFILQRREMR YAFSWRQD
Length:1,028
Mass (Da):116,441
Last modified:May 29, 2007 - v1
Checksum:i57258FFE65A78032
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000653 Genomic DNA. Translation: ABP59612.1.
RefSeqiWP_012016333.1. NC_009436.1.

Genome annotation databases

EnsemblBacteriaiABP59612; ABP59612; Ent638_0928.
KEGGient:Ent638_0928.
PATRICi20411432. VBIEntSp101211_1065.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000653 Genomic DNA. Translation: ABP59612.1.
RefSeqiWP_012016333.1. NC_009436.1.

3D structure databases

ProteinModelPortaliA4W7D2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi399742.Ent638_0928.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Proteomic databases

PRIDEiA4W7D2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABP59612; ABP59612; Ent638_0928.
KEGGient:Ent638_0928.
PATRICi20411432. VBIEntSp101211_1065.

Phylogenomic databases

eggNOGiENOG4105CNT. Bacteria.
COG3250. LUCA.
HOGENOMiHOG000252443.
KOiK01190.
OMAiGMVPMSR.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. GH-type_carb-bd.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006103. Glyco_hydro_2_cat.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR032312. LacZ_4.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF16353. DUF4981. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBGAL_ENT38
AccessioniPrimary (citable) accession number: A4W7D2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 29, 2007
Last modified: November 2, 2016
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.