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A4W5G8

- APHA_ENT38

UniProt

A4W5G8 - APHA_ENT38

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Protein

Class B acid phosphatase

Gene

aphA

Organism
Enterobacter sp. (strain 638)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Dephosphorylates several organic phosphate monoesters. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds (By similarity).By similarity

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.By similarity

Cofactori

Binds 1 magnesium ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei69 – 691NucleophileBy similarity
Metal bindingi69 – 691MagnesiumBy similarity
Active sitei71 – 711Proton donorBy similarity
Metal bindingi71 – 711Magnesium; via carbonyl oxygenBy similarity
Binding sitei177 – 1771SubstrateBy similarity
Metal bindingi192 – 1921MagnesiumBy similarity

GO - Molecular functioni

  1. acid phosphatase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciESP399742:GJ0E-269-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Class B acid phosphataseBy similarity (EC:3.1.3.2ImportedBy similarity)
Short name:
CBAPBy similarity
Gene namesi
Name:aphABy similarity
Ordered Locus Names:Ent638_0259
OrganismiEnterobacter sp. (strain 638)
Taxonomic identifieri399742 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacter
ProteomesiUP000000230: Chromosome

Subcellular locationi

Periplasm By similarity

GO - Cellular componenti

  1. outer membrane-bounded periplasmic space Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Chaini26 – 237212Class B acid phosphataseSequence AnalysisPRO_5000237561Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi399742.Ent638_0259.

Structurei

3D structure databases

ProteinModelPortaliA4W5G8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni137 – 1382Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the class B bacterial acid phosphatase family.By similarity

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3700.
HOGENOMiHOG000270623.
KOiK03788.
OMAiPEFWEKM.
OrthoDBiEOG6HTNVZ.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR005519. Acid_phosphat_B.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view]
PfamiPF03767. Acid_phosphat_B. 1 hit.
[Graphical view]
PIRSFiPIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01672. AphA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A4W5G8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRKITLALSA ACLLFSLNNA VVARASAPTP LYTGTTAAIL AEQAPIHWVS
60 70 80 90 100
VAQIENSLMG RAPMAVGFDI DDTVLFSSPG FWRGKKTFSP ASEEYLKNPE
110 120 130 140 150
FWEKMNNGWD EFSIPKEVGR ALIAMHVKRG DSIYFVTGRS QTKTETVSKT
160 170 180 190 200
LQGDFAIPSA NMNPVIFAGD KAGQNTKTQW LEQKNIKVFY GDSDNDITAA
210 220 230
RDVGARGIRV LRASNSTYRP LPMAGAFGEE VIVNSEY
Length:237
Mass (Da):25,963
Last modified:May 29, 2007 - v1
Checksum:i9D4F8608932A1E42
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000653 Genomic DNA. Translation: ABP58948.1.
RefSeqiWP_011915521.1. NC_009436.1.
YP_001174999.1. NC_009436.1.

Genome annotation databases

EnsemblBacteriaiABP58948; ABP58948; Ent638_0259.
GeneIDi5112865.
KEGGient:Ent638_0259.
PATRICi20410040. VBIEntSp101211_0385.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000653 Genomic DNA. Translation: ABP58948.1 .
RefSeqi WP_011915521.1. NC_009436.1.
YP_001174999.1. NC_009436.1.

3D structure databases

ProteinModelPortali A4W5G8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 399742.Ent638_0259.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABP58948 ; ABP58948 ; Ent638_0259 .
GeneIDi 5112865.
KEGGi ent:Ent638_0259.
PATRICi 20410040. VBIEntSp101211_0385.

Phylogenomic databases

eggNOGi COG3700.
HOGENOMi HOG000270623.
KOi K03788.
OMAi PEFWEKM.
OrthoDBi EOG6HTNVZ.

Enzyme and pathway databases

BioCyci ESP399742:GJ0E-269-MONOMER.

Family and domain databases

Gene3Di 3.40.50.1000. 1 hit.
InterProi IPR005519. Acid_phosphat_B.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view ]
Pfami PF03767. Acid_phosphat_B. 1 hit.
[Graphical view ]
PIRSFi PIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMi SSF56784. SSF56784. 1 hit.
TIGRFAMsi TIGR01672. AphA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the plant growth promoting endophytic bacterium Enterobacter sp. 638."
    Taghavi S., van der Lelie D., Hoffman A., Zhang Y.B., Walla M.D., Vangronsveld J., Newman L., Monchy S.
    PLoS Genet. 6:E1000943-E1000943(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 638.

Entry informationi

Entry nameiAPHA_ENT38
AccessioniPrimary (citable) accession number: A4W5G8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: May 29, 2007
Last modified: October 1, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3