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A4W5G8

- APHA_ENT38

UniProt

A4W5G8 - APHA_ENT38

Protein

Class B acid phosphatase

Gene

aphA

Organism
Enterobacter sp. (strain 638)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 43 (01 Oct 2014)
      Sequence version 1 (29 May 2007)
      Previous versions | rss
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    Functioni

    Dephosphorylates several organic phosphate monoesters. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds By similarity.By similarity

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.By similarity

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei69 – 691NucleophileBy similarity
    Metal bindingi69 – 691MagnesiumBy similarity
    Active sitei71 – 711Proton donorBy similarity
    Metal bindingi71 – 711Magnesium; via carbonyl oxygenBy similarity
    Binding sitei177 – 1771SubstrateBy similarity
    Metal bindingi192 – 1921MagnesiumBy similarity

    GO - Molecular functioni

    1. acid phosphatase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciESP399742:GJ0E-269-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Class B acid phosphataseBy similarity (EC:3.1.3.2ImportedBy similarity)
    Short name:
    CBAPBy similarity
    Gene namesi
    Name:aphABy similarity
    Ordered Locus Names:Ent638_0259
    OrganismiEnterobacter sp. (strain 638)
    Taxonomic identifieri399742 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacter
    ProteomesiUP000000230: Chromosome

    Subcellular locationi

    Periplasm By similarity

    GO - Cellular componenti

    1. outer membrane-bounded periplasmic space Source: InterPro

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525Sequence AnalysisAdd
    BLAST
    Chaini26 – 237212Class B acid phosphataseSequence AnalysisPRO_5000237561Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi399742.Ent638_0259.

    Structurei

    3D structure databases

    ProteinModelPortaliA4W5G8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni137 – 1382Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the class B bacterial acid phosphatase family.By similarity

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG3700.
    HOGENOMiHOG000270623.
    KOiK03788.
    OMAiPEFWEKM.
    OrthoDBiEOG6HTNVZ.

    Family and domain databases

    Gene3Di3.40.50.1000. 1 hit.
    InterProiIPR005519. Acid_phosphat_B.
    IPR023214. HAD-like_dom.
    IPR010025. HAD-SF_ppase_IIIB_AphA.
    [Graphical view]
    PfamiPF03767. Acid_phosphat_B. 1 hit.
    [Graphical view]
    PIRSFiPIRSF017818. Acid_Ptase_B. 1 hit.
    SUPFAMiSSF56784. SSF56784. 1 hit.
    TIGRFAMsiTIGR01672. AphA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A4W5G8-1 [UniParc]FASTAAdd to Basket

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    MRKITLALSA ACLLFSLNNA VVARASAPTP LYTGTTAAIL AEQAPIHWVS    50
    VAQIENSLMG RAPMAVGFDI DDTVLFSSPG FWRGKKTFSP ASEEYLKNPE 100
    FWEKMNNGWD EFSIPKEVGR ALIAMHVKRG DSIYFVTGRS QTKTETVSKT 150
    LQGDFAIPSA NMNPVIFAGD KAGQNTKTQW LEQKNIKVFY GDSDNDITAA 200
    RDVGARGIRV LRASNSTYRP LPMAGAFGEE VIVNSEY 237
    Length:237
    Mass (Da):25,963
    Last modified:May 29, 2007 - v1
    Checksum:i9D4F8608932A1E42
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000653 Genomic DNA. Translation: ABP58948.1.
    RefSeqiWP_011915521.1. NC_009436.1.
    YP_001174999.1. NC_009436.1.

    Genome annotation databases

    EnsemblBacteriaiABP58948; ABP58948; Ent638_0259.
    GeneIDi5112865.
    KEGGient:Ent638_0259.
    PATRICi20410040. VBIEntSp101211_0385.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000653 Genomic DNA. Translation: ABP58948.1 .
    RefSeqi WP_011915521.1. NC_009436.1.
    YP_001174999.1. NC_009436.1.

    3D structure databases

    ProteinModelPortali A4W5G8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 399742.Ent638_0259.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABP58948 ; ABP58948 ; Ent638_0259 .
    GeneIDi 5112865.
    KEGGi ent:Ent638_0259.
    PATRICi 20410040. VBIEntSp101211_0385.

    Phylogenomic databases

    eggNOGi COG3700.
    HOGENOMi HOG000270623.
    KOi K03788.
    OMAi PEFWEKM.
    OrthoDBi EOG6HTNVZ.

    Enzyme and pathway databases

    BioCyci ESP399742:GJ0E-269-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.1000. 1 hit.
    InterProi IPR005519. Acid_phosphat_B.
    IPR023214. HAD-like_dom.
    IPR010025. HAD-SF_ppase_IIIB_AphA.
    [Graphical view ]
    Pfami PF03767. Acid_phosphat_B. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF017818. Acid_Ptase_B. 1 hit.
    SUPFAMi SSF56784. SSF56784. 1 hit.
    TIGRFAMsi TIGR01672. AphA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the plant growth promoting endophytic bacterium Enterobacter sp. 638."
      Taghavi S., van der Lelie D., Hoffman A., Zhang Y.B., Walla M.D., Vangronsveld J., Newman L., Monchy S.
      PLoS Genet. 6:E1000943-E1000943(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 638.

    Entry informationi

    Entry nameiAPHA_ENT38
    AccessioniPrimary (citable) accession number: A4W5G8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 25, 2012
    Last sequence update: May 29, 2007
    Last modified: October 1, 2014
    This is version 43 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3