Reviewed, UniProtKB/Swiss-Prot A4W5F4 (PLSB_ENT38)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate acyltransferase
      Short name=GPAT
    EC=2.3.1.15

Gene names

Name:	plsB
Ordered Locus Names:	Ent638_0245

Organism

Enterobacter sp. (strain 638) [Complete proteome] [HAMAP]

Taxonomic identifier

399742 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Enterobacter

Protein attributes

Sequence length	806 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. HAMAP MF_00393
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. HAMAP MF_00393
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell membrane Membrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 806	806	Glycerol-3-phosphate acyltransferase HAMAP MF_00393		PRO_1000060777
Regions
	Motif	305 – 310	6	HXXXXD motif HAMAP MF_00393

Sequences

	Sequence		Length	Mass (Da)
	A4W5F4-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 5A5DADC9187EF084	FASTA	806	91,643
	10 20 30 40 50 60 MSGWPRIYYK LLNLPLSVLV KSKSIPAEPA LELGLDTSRP IMYVLPYNSK ADLLTLRAQC 70 80 90 100 110 120 LAHDLPDPLE PLEIDGTLLP RYVFIHGGPR VFTYYTPKEE SIKLFHDYLD LHRSNPDLDV 130 140 150 160 170 180 QMVPVSVMFG RRPGRQKGEE NPPLRMLNGV QKFFAVSWLG RDSFVRFSPS VSLRHMADEH 190 200 210 220 230 240 GTDKIIAQKL ARVARMHFAR QRLAAVGPRL PARQDLFNKL LASKAIARAV EDEARSKKIS 250 260 270 280 290 300 HEKAQQNAIA LMEEIAANFS YEMIRLSDRI LSFTWNRLYQ GINVHNAERV RQLAHDGHEI 310 320 330 340 350 360 VYVPCHRSHM DYLLLSYVLY HQGLVPPHIA AGINLNFWPA GPIFRRLGAF FIRRTFKGNK 370 380 390 400 410 420 LYSTVFREYL GELFSRGYSV EYFVEGGRSR TGRLLDPKTG TLSMTIQAML RGGTRPITLV 430 440 450 460 470 480 PIYIGYEHVM EVGTYAKELR GATKEKESLP QMLRGLSKLR NLGQGYVNFG EPMPLMTYLN 490 500 510 520 530 540 HHVPEWRESI DPIEAIRPAW LTPTVNNIAA DLMVRINNAG AANAMNLCCT ALLASRQRSL 550 560 570 580 590 600 TREQLTEQLD CYLDMMRHVP YSTDSTVPSV TAGQLIDHAL QMNKFEVEKD TIGDIIILPR 610 620 630 640 650 660 EQAVLMTYYR NNIAHMLMLP SLMAAIITQH RRISRQEILR HVEVLYPMLK AELFLRWSKD 670 680 690 700 710 720 ELAAELDKLT EELLRQGLIA VKGDELHINP SRSRTLQLLA AGARETLQRY AITFWLLSAN 730 740 750 760 770 780 PSINRGTLEK ESRTLAQRLS VLHGINAPEF FDKAVFSSLV LTLRDEGYIS DTGDAEPGET 790 800 MKVYQMLAEL ITSDVRLTIE SATQDE

References

[1]

"Complete sequence of chromosome of Enterobacter sp. 638." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Taghavi S., Newman L., Vangronsveld J., van der Lelie D., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000653 Genomic DNA. Translation: ABP58934.1.
RefSeq	YP_001174985.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4W5F4.
Genome annotation databases
GeneID	5113488.
GenomeReviews	Gene locus Ent638_0245 in contig CP000653_GR.
KEGG	ent:Ent638_0245.
NMPDR	fig|399742.4.peg.324.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VPVYIGY.
Family and domain databases
HAMAP	MF_00393. [Tree]
InterPro	IPR002123. Acyltransferase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLSB_ENT38

Accession

Primary (citable) accession number: A4W5F4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	May 29, 2007
Last modified:	November 3, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)