Reviewed, UniProtKB/Swiss-Prot A4W5D0 (ACEK_ENT38)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase kinase/phosphatase
      Short name=IDH kinase/phosphatase
      Short name=IDHK/P
    EC=2.7.11.5
    EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	Ent638_0220

Organism

Enterobacter sp. (strain 638) [Complete proteome] [HAMAP]

Taxonomic identifier

399742 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Enterobacter

Protein attributes

Sequence length	573 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity.
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the aceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding NADP Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: HAMAP phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 573	573	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_0000315265
Regions
	Nucleotide binding	315 – 321	7	ATP By similarity
Sites
	Active site	371	1	By similarity
	Binding site	336	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4W5D0-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 58BC9849FC719780	FASTA	573	66,734
	10 20 30 40 50 60 MSRGLELLIA QTILQGFDAQ YGRFLEVTSG AQQRFEHADW HAVQQAMKQR IHLYDHHVGL 70 80 90 100 110 120 VVEQLRCITE GKSTDVDFLL RVKEHYTHLL PDYPRFEIAE SFFNSVYCRL FDHRSLSPER 130 140 150 160 170 180 LFIFSSQPAR RFRAIPRPLA KDFFPEKGWE KALNNVLSDL PLRLPWQNKT RDVGYISAHL 190 200 210 220 230 240 VETLGEETLR HSHLQVANEL FFRNKAAWLV GKLITPDATL PFLLPIHRSD DGELFVDTCL 250 260 270 280 290 300 TTSAEASIVF GFARSYFMVY APFPAALVEW LREILPVKTT AELYMAIGCQ KHAKTESYRE 310 320 330 340 350 360 YLYYITSTDE QFIEAPGIRG MVMLVFTLPG FDRVFKVIKD QFAPQKEMTA AHVRACYQLV 370 380 390 400 410 420 KEHDRVGRMA DTQEFENFVL DKRQIDPTLM ALLLQEAPDK ITDLGDKIAI SHLYIERRMV 430 440 450 460 470 480 PLNIWLEQVE GAQLRDAIEE YGNAIRQLAA ANIFPGDMLF KNFGVTRHGR VVFYDYDEIC 490 500 510 520 530 540 YMTEVNFRTI PAARYPEDEL ASEPWYSVSP GDVFPEEFRH WLCADPRIGP LFEEMHDDLF 550 560 570 RADYWRSLQT RIKEGHVEDV YAYRKRQRFC LRG

References

[1]

"Complete sequence of chromosome of Enterobacter sp. 638." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Taghavi S., Newman L., Vangronsveld J., van der Lelie D., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000653 Genomic DNA. Translation: ABP58910.1.
RefSeq	YP_001174961.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4W5D0.
Genome annotation databases
GeneID	5113152.
GenomeReviews	Gene locus Ent638_0220 in contig CP000653_GR.
KEGG	ent:Ent638_0220.
NMPDR	fig|399742.4.peg.302.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	FYRDFLH.
Family and domain databases
HAMAP	MF_00747. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACEK_ENT38

Accession

Primary (citable) accession number: A4W5D0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 29, 2007
Last modified:	November 3, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)