Reviewed, UniProtKB/Swiss-Prot A4W577 (GHRB_ENT38)

Names and origin

Protein names

Recommended name:
    Glyoxylate/hydroxypyruvate reductase B
    EC=1.1.1.79
    EC=1.1.1.81

Gene names

Name:	ghrB
Ordered Locus Names:	Ent638_0167

Organism

Enterobacter sp. (strain 638) [Complete proteome] [HAMAP]

Taxonomic identifier

399742 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Enterobacter

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively By similarity.
Catalytic activity	Glycolate + NADP+ = glyoxylate + NADPH. HAMAP MF_01667 D-glycerate + NAD(P)+ = hydroxypyruvate + NAD(P)H. HAMAP MF_01667
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrB subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro glyoxylate reductase (NADP) activity Inferred from electronic annotation. Source: HAMAP hydroxypyruvate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	Glyoxylate/hydroxypyruvate reductase B HAMAP MF_01667		PRO_0000348381
Sites
	Active site	237	1	By similarity
	Active site	266	1	By similarity
	Active site	285	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4W577-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 1323B43A8356FC68	FASTA	324	35,661
	10 20 30 40 50 60 MKPSVILYKT LPDDLQKRLE EHFTVTQMKN LNPETVAEHA DAFASAAGLL GSSEKVDTAL 70 80 90 100 110 120 LEKMPKLRAT STISVGYDNF DVDALNTRKI LLMHTPYALT ETVADTLMAL VLSTARRVVE 130 140 150 160 170 180 VAERVKAGEW TKSIGPDWFG VDVHGKTLGI VGMGRIGLAL AQRAHFGFNM PILYNARRHH 190 200 210 220 230 240 SEAEERFEAR YCELETLLQE ADYVCLILPL TDETHHLIGK AEFEKMKKSA IFINAGRGPV 250 260 270 280 290 300 VDEKALIEAL QKGEIHAAGL DVFEQEPLPV DSPLLTMSNV VSLPHIGSAT HETRYNMAAT 310 320 AVDNLINALN GNVEKNCVNP HVNA

References

[1]

"Complete sequence of chromosome of Enterobacter sp. 638." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Taghavi S., Newman L., Vangronsveld J., van der Lelie D., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000653 Genomic DNA. Translation: ABP58857.1.
RefSeq	YP_001174908.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5113265.
GenomeReviews	Gene locus Ent638_0167 in contig CP000653_GR.
KEGG	ent:Ent638_0167.
NMPDR	fig|399742.4.peg.248.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A4W577. MARCAVE.
Family and domain databases
HAMAP	MF_01667. [Tree]
InterPro	IPR006139. D-isomer_2_OHA_DH. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. False negative. PS00670. D_2_HYDROXYACID_DH_2. False negative. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GHRB_ENT38

Accession

Primary (citable) accession number: A4W577

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	May 29, 2007
Last modified:	June 16, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)