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A4W4F3 (SYL_STRS2) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:SSU98_2084
OrganismStreptococcus suis (strain 98HAH33) [Complete proteome] [HAMAP]
Taxonomic identifier391296 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length833 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence caution

The sequence ABP93242.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 833833Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334827

Regions

Motif41 – 5212"HIGH" region HAMAP-Rule MF_00049
Motif610 – 6145"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6131ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A4W4F3 [UniParc].

Last modified May 20, 2008. Version 2.
Checksum: 4E2B5A4315886AEB

FASTA83394,037
        10         20         30         40         50         60 
MSFYNHKEIE PKWQEFWAKN HTFKTGTDAE KPNFYALDMF PYPSGAGLHV GHPEGYTATD 

        70         80         90        100        110        120 
ILSRYKRAQG YNVLHPMGWD AFGLPAEQYA MDTGNDPADF TAENIANFKR QINALGFSYD 

       130        140        150        160        170        180 
WDREVNTTDP NYYKWTQWIF TKLYEKGLAY EAEVPVNWVE ELGTAIANEE VLPDGTSERG 

       190        200        210        220        230        240 
GYPVVRKPMR QWMLKITAYA ERLLNDLEEV DWPESIKDMQ RNWIGKSTGA NVTFKIKDTD 

       250        260        270        280        290        300 
KDFTVFTTRP DTLFGATYAV LAPEHDLVDS ITSAEQAEAV AEYKRQASLK SDLARTDLAK 

       310        320        330        340        350        360 
DKTGVWTGAY AINPVNGKEI PIWIADYVLA SYGTGAIMAV PAHDERDWEF AKQFGLDIIP 

       370        380        390        400        410        420 
VLEGGNVEEA PYTEDGAHIN SDFLDGLNKE EAIAKMVAWL EENGVGQEKI SYRLRDWLFS 

       430        440        450        460        470        480 
RQRYWGEPIP IIHWEDGTST AVPENELPLV LPKTSDIKPS GTGESPLANL TDWLEVVRED 

       490        500        510        520        530        540 
GVKGRRETNT MPQWAGSSWY YLRYIDPHND EKLADEDLLK AWLPVDIYIG GAEHAVLHLL 

       550        560        570        580        590        600 
YARFWHKFLY DLGVVPTKEP FQKLFNQGMI LGTSYRDSRG ALVATDKVEK RDGSFFHMET 

       610        620        630        640        650        660 
GEELEQAPAK MSKSLKNVVN PDDVVEQFGA DTLRVYEMFM GPLDASIAWS EEGLEGSRKF 

       670        680        690        700        710        720 
LDRVYRLLTT KELVAENSGA LDKVYNETVK TVTEHIEDLK FNTAIAQLMI FVNAANKEDK 

       730        740        750        760        770        780 
LYVDYAKGFV QLIAPFAPHL AEELWQGLAN TGQSISYVAW PTYDESKLVE SEVEIVVQIK 

       790        800        810        820        830 
GKVKARLTVA KDLAPAELEK VALADEKVQA EIAGQTVVKV ISVPNKLVNI VVK 

« Hide

References

[1]"A glimpse of streptococcal toxic shock syndrome from comparative genomics of S. suis 2 Chinese isolates."
Chen C., Tang J., Dong W., Wang C., Feng Y., Wang J., Zheng F., Pan X., Liu D., Li M., Song Y., Zhu X., Sun H., Feng T., Guo Z., Ju A., Ge J., Dong Y. expand/collapse author list , Sun W., Jiang Y., Wang J., Yan J., Yang H., Wang X., Gao G.F., Yang R., Wang J., Yu J.
PLoS ONE 2:E315-E315(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 98HAH33.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000408 Genomic DNA. Translation: ABP93242.1. Different initiation.
RefSeqYP_001201642.1. NC_009443.1.

3D structure databases

ProteinModelPortalA4W4F3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING391296.SSU98_2084.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABP93242; ABP93242; SSU98_2084.
GeneID5102124.
KEGGssv:SSU98_2084.
PATRIC19780390. VBIStrSui72275_2050.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycSSUI391296:GI2E-2142-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_STRS2
AccessionPrimary (citable) accession number: A4W4F3
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: June 11, 2014
This is version 56 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries