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A4W3A5

- RLMN_STRS2

UniProt

A4W3A5 - RLMN_STRS2

Protein

Probable dual-specificity RNA methyltransferase RlmN

Gene

rlmN

Organism
Streptococcus suis (strain 98HAH33)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 55 (01 Oct 2014)
      Sequence version 1 (29 May 2007)
      Previous versions | rss
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    Functioni

    Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.UniRule annotation

    Catalytic activityi

    2 S-adenosyl-L-methionine + adenine(2503) in 23S rRNA = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine(2503) in 23S rRNA.UniRule annotation
    2 S-adenosyl-L-methionine + adenine(37) in tRNA = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine(37) in tRNA.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei91 – 911Proton acceptorUniRule annotation
    Metal bindingi111 – 1111Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi115 – 1151Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi118 – 1181Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
    Binding sitei195 – 1951S-adenosyl-L-methionineUniRule annotation
    Binding sitei296 – 2961S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation
    Active sitei340 – 3401S-methylcysteine intermediateUniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW
    3. rRNA (adenine-C2-)-methyltransferase activity Source: UniProtKB-HAMAP
    4. rRNA binding Source: UniProtKB-HAMAP
    5. tRNA (adenine-C2-)-methyltransferase activity Source: UniProtKB-HAMAP
    6. tRNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. rRNA base methylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    rRNA processing, tRNA processing

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciSSUI391296:GI2E-1742-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable dual-specificity RNA methyltransferase RlmNUniRule annotation (EC:2.1.1.-UniRule annotation, EC:2.1.1.192UniRule annotation)
    Alternative name(s):
    23S rRNA (adenine(2503)-C(2))-methyltransferaseUniRule annotation
    23S rRNA m2A2503 methyltransferaseUniRule annotation
    Ribosomal RNA large subunit methyltransferase NUniRule annotation
    tRNA (adenine(37)-C(2))-methyltransferaseUniRule annotation
    tRNA m2A37 methyltransferaseUniRule annotation
    Gene namesi
    Name:rlmNUniRule annotation
    Ordered Locus Names:SSU98_1686
    OrganismiStreptococcus suis (strain 98HAH33)
    Taxonomic identifieri391296 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
    ProteomesiUP000000244: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 370370Probable dual-specificity RNA methyltransferase RlmNPRO_0000350469Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi104 ↔ 340(transient)UniRule annotation

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi391296.SSU98_1686.

    Structurei

    3D structure databases

    ProteinModelPortaliA4W3A5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni163 – 1642S-adenosyl-L-methionine bindingUniRule annotation
    Regioni218 – 2203S-adenosyl-L-methionine bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. RlmN family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0820.
    HOGENOMiHOG000217991.
    KOiK06941.
    OMAiHEGSKFE.
    OrthoDBiEOG6DJZ2N.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01849. RNA_methyltr_RlmN.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR027492. RNA_MTrfase_RlmN.
    IPR004383. rRNA_lsu_MTrfase_RlmN/Cfr.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR30544. PTHR30544. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006004. CHP00048. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00048. TIGR00048. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A4W3A5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKPSIYAFSQ ANLVDWILEN GEKKFRATQI WEWLYRSRVQ SFAEMTNLPK    50
    SLIEKLEEHF VVNPLKQRIV QESKDGTIKY LFELPDGMLI ETVLMHQHYG 100
    LSVCVTTQVG CNIGCTFCAS GLIPKQRDLT SGEIVAQIML VQKYLDERNQ 150
    NERVSHIVVM GIGEPLDNYD NVMTFLRVVN DDKGLAIGAR HITVSTSGLA 200
    PKIREFAREG VQVNLAVSLH APNNDLRSSI MRINRKFPIE VLFEAIEDYI 250
    KVTNRRVTFE YIMLNEVNDG VEQAQELADL TKNIRKLSYI NLIPYNPVSE 300
    HDQYSRSTKE RTLAFFDVLK KNGVNCVVRQ EHGTDIDAAC GQLRSNTLKK 350
    DREKARARIA AAKAKAGIRA 370
    Length:370
    Mass (Da):42,060
    Last modified:May 29, 2007 - v1
    Checksum:iE90BAF008866B358
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000408 Genomic DNA. Translation: ABP92844.1.
    RefSeqiYP_001201244.1. NC_009443.1.

    Genome annotation databases

    EnsemblBacteriaiABP92844; ABP92844; SSU98_1686.
    GeneIDi5103113.
    KEGGissv:SSU98_1686.
    PATRICi19779594. VBIStrSui72275_1654.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000408 Genomic DNA. Translation: ABP92844.1 .
    RefSeqi YP_001201244.1. NC_009443.1.

    3D structure databases

    ProteinModelPortali A4W3A5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 391296.SSU98_1686.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABP92844 ; ABP92844 ; SSU98_1686 .
    GeneIDi 5103113.
    KEGGi ssv:SSU98_1686.
    PATRICi 19779594. VBIStrSui72275_1654.

    Phylogenomic databases

    eggNOGi COG0820.
    HOGENOMi HOG000217991.
    KOi K06941.
    OMAi HEGSKFE.
    OrthoDBi EOG6DJZ2N.

    Enzyme and pathway databases

    BioCyci SSUI391296:GI2E-1742-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01849. RNA_methyltr_RlmN.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR027492. RNA_MTrfase_RlmN.
    IPR004383. rRNA_lsu_MTrfase_RlmN/Cfr.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR30544. PTHR30544. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006004. CHP00048. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00048. TIGR00048. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A glimpse of streptococcal toxic shock syndrome from comparative genomics of S. suis 2 Chinese isolates."
      Chen C., Tang J., Dong W., Wang C., Feng Y., Wang J., Zheng F., Pan X., Liu D., Li M., Song Y., Zhu X., Sun H., Feng T., Guo Z., Ju A., Ge J., Dong Y.
      , Sun W., Jiang Y., Wang J., Yan J., Yang H., Wang X., Gao G.F., Yang R., Wang J., Yu J.
      PLoS ONE 2:E315-E315(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 98HAH33.

    Entry informationi

    Entry nameiRLMN_STRS2
    AccessioniPrimary (citable) accession number: A4W3A5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 2, 2008
    Last sequence update: May 29, 2007
    Last modified: October 1, 2014
    This is version 55 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue.UniRule annotation

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3