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A4W3A5 (RLMN_STRS2) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable dual-specificity RNA methyltransferase RlmN

EC=2.1.1.-
EC=2.1.1.192
Alternative name(s):
23S rRNA (adenine(2503)-C(2))-methyltransferase
23S rRNA m2A2503 methyltransferase
Ribosomal RNA large subunit methyltransferase N
tRNA (adenine(37)-C(2))-methyltransferase
tRNA m2A37 methyltransferase
Gene names
Name:rlmN
Ordered Locus Names:SSU98_1686
OrganismStreptococcus suis (strain 98HAH33) [Complete proteome] [HAMAP]
Taxonomic identifier391296 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length370 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs By similarity. HAMAP-Rule MF_01849

Catalytic activity

2 S-adenosyl-L-methionine + adenine(2503) in 23S rRNA = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine(2503) in 23S rRNA. HAMAP-Rule MF_01849

2 S-adenosyl-L-methionine + adenine37 in tRNA = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine37 in tRNA. HAMAP-Rule MF_01849

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_01849.

Miscellaneous

Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue By similarity.

Sequence similarities

Belongs to the radical SAM superfamily. RlmN family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 370370Probable dual-specificity RNA methyltransferase RlmN HAMAP-Rule MF_01849
PRO_0000350469

Regions

Region163 – 1642S-adenosyl-L-methionine binding By similarity
Region218 – 2203S-adenosyl-L-methionine binding By similarity

Sites

Active site911Proton acceptor Potential
Active site3401S-methylcysteine intermediate By similarity
Metal binding1111Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding1151Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding1181Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Binding site1951S-adenosyl-L-methionine By similarity
Binding site2961S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen By similarity

Amino acid modifications

Disulfide bond104 ↔ 340(transient) By similarity

Sequences

Sequence LengthMass (Da)Tools
A4W3A5 [UniParc].

Last modified May 29, 2007. Version 1.
Checksum: E90BAF008866B358

FASTA37042,060
        10         20         30         40         50         60 
MKPSIYAFSQ ANLVDWILEN GEKKFRATQI WEWLYRSRVQ SFAEMTNLPK SLIEKLEEHF 

        70         80         90        100        110        120 
VVNPLKQRIV QESKDGTIKY LFELPDGMLI ETVLMHQHYG LSVCVTTQVG CNIGCTFCAS 

       130        140        150        160        170        180 
GLIPKQRDLT SGEIVAQIML VQKYLDERNQ NERVSHIVVM GIGEPLDNYD NVMTFLRVVN 

       190        200        210        220        230        240 
DDKGLAIGAR HITVSTSGLA PKIREFAREG VQVNLAVSLH APNNDLRSSI MRINRKFPIE 

       250        260        270        280        290        300 
VLFEAIEDYI KVTNRRVTFE YIMLNEVNDG VEQAQELADL TKNIRKLSYI NLIPYNPVSE 

       310        320        330        340        350        360 
HDQYSRSTKE RTLAFFDVLK KNGVNCVVRQ EHGTDIDAAC GQLRSNTLKK DREKARARIA 

       370 
AAKAKAGIRA 

« Hide

References

[1]"A glimpse of streptococcal toxic shock syndrome from comparative genomics of S. suis 2 Chinese isolates."
Chen C., Tang J., Dong W., Wang C., Feng Y., Wang J., Zheng F., Pan X., Liu D., Li M., Song Y., Zhu X., Sun H., Feng T., Guo Z., Ju A., Ge J., Dong Y. expand/collapse author list , Sun W., Jiang Y., Wang J., Yan J., Yang H., Wang X., Gao G.F., Yang R., Wang J., Yu J.
PLoS ONE 2:E315-E315(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 98HAH33.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000408 Genomic DNA. Translation: ABP92844.1.
RefSeqYP_001201244.1. NC_009443.1.

3D structure databases

ProteinModelPortalA4W3A5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING391296.SSU98_1686.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABP92844; ABP92844; SSU98_1686.
GeneID5103113.
KEGGssv:SSU98_1686.
PATRIC19779594. VBIStrSui72275_1654.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0820.
HOGENOMHOG000217991.
KOK06941.
OMAHEGSKFE.
OrthoDBEOG6DJZ2N.

Enzyme and pathway databases

BioCycSSUI391296:GI2E-1742-MONOMER.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01849. RNA_methyltr_RlmN.
InterProIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR027492. RNA_MTrfase_RlmN.
IPR004383. rRNA_lsu_MTrfase_RlmN/Cfr.
IPR007197. rSAM.
[Graphical view]
PANTHERPTHR30544. PTHR30544. 1 hit.
PfamPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF006004. CHP00048. 1 hit.
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00048. TIGR00048. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRLMN_STRS2
AccessionPrimary (citable) accession number: A4W3A5
Entry history
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: May 29, 2007
Last modified: May 14, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families