Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable dual-specificity RNA methyltransferase RlmN

Gene

rlmN

Organism
Streptococcus suis (strain 98HAH33)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.UniRule annotation

Miscellaneous

Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue.UniRule annotation

Catalytic activityi

2 S-adenosyl-L-methionine + adenine(2503) in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine(2503) in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation
2 S-adenosyl-L-methionine + adenine(37) in tRNA + 2 reduced [2Fe-2S] ferredoxin = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine(37) in tRNA + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei91Proton acceptorUniRule annotation1
Metal bindingi111Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi115Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi118Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Binding sitei195S-adenosyl-L-methionineUniRule annotation1
Binding sitei296S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation1
Active sitei340S-methylcysteine intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processrRNA processing, tRNA processing
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Probable dual-specificity RNA methyltransferase RlmNUniRule annotation (EC:2.1.1.192UniRule annotation)
Alternative name(s):
23S rRNA (adenine(2503)-C(2))-methyltransferaseUniRule annotation
23S rRNA m2A2503 methyltransferaseUniRule annotation
Ribosomal RNA large subunit methyltransferase NUniRule annotation
tRNA (adenine(37)-C(2))-methyltransferaseUniRule annotation
tRNA m2A37 methyltransferaseUniRule annotation
Gene namesi
Name:rlmNUniRule annotation
Ordered Locus Names:SSU98_1686
OrganismiStreptococcus suis (strain 98HAH33)
Taxonomic identifieri391296 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003504691 – 370Probable dual-specificity RNA methyltransferase RlmNAdd BLAST370

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi104 ↔ 340(transient)UniRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiA4W3A5

Structurei

3D structure databases

ProteinModelPortaliA4W3A5
SMRiA4W3A5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni163 – 164S-adenosyl-L-methionine bindingUniRule annotation2
Regioni218 – 220S-adenosyl-L-methionine bindingUniRule annotation3

Sequence similaritiesi

Belongs to the radical SAM superfamily. RlmN family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000217991
KOiK06941
OMAiKVVFMGM

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01849 RNA_methyltr_RlmN, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR027492 RNA_MTrfase_RlmN
IPR004383 rRNA_lsu_MTrfase_RlmN/Cfr
IPR007197 rSAM
PANTHERiPTHR30544 PTHR30544, 1 hit
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF006004 CHP00048, 1 hit
SFLDiSFLDF00275 adenosine_C2_methyltransferase, 1 hit
SFLDS00029 Radical_SAM, 1 hit
TIGRFAMsiTIGR00048 rRNA_mod_RlmN, 1 hit

Sequencei

Sequence statusi: Complete.

A4W3A5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPSIYAFSQ ANLVDWILEN GEKKFRATQI WEWLYRSRVQ SFAEMTNLPK
60 70 80 90 100
SLIEKLEEHF VVNPLKQRIV QESKDGTIKY LFELPDGMLI ETVLMHQHYG
110 120 130 140 150
LSVCVTTQVG CNIGCTFCAS GLIPKQRDLT SGEIVAQIML VQKYLDERNQ
160 170 180 190 200
NERVSHIVVM GIGEPLDNYD NVMTFLRVVN DDKGLAIGAR HITVSTSGLA
210 220 230 240 250
PKIREFAREG VQVNLAVSLH APNNDLRSSI MRINRKFPIE VLFEAIEDYI
260 270 280 290 300
KVTNRRVTFE YIMLNEVNDG VEQAQELADL TKNIRKLSYI NLIPYNPVSE
310 320 330 340 350
HDQYSRSTKE RTLAFFDVLK KNGVNCVVRQ EHGTDIDAAC GQLRSNTLKK
360 370
DREKARARIA AAKAKAGIRA
Length:370
Mass (Da):42,060
Last modified:May 29, 2007 - v1
Checksum:iE90BAF008866B358
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000408 Genomic DNA Translation: ABP92844.1

Genome annotation databases

EnsemblBacteriaiABP92844; ABP92844; SSU98_1686
KEGGissv:SSU98_1686

Similar proteinsi

Entry informationi

Entry nameiRLMN_STRS2
AccessioniPrimary (citable) accession number: A4W3A5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: May 29, 2007
Last modified: May 23, 2018
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health