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A4W3A5

- RLMN_STRS2

UniProt

A4W3A5 - RLMN_STRS2

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Protein
Probable dual-specificity RNA methyltransferase RlmN
Gene
rlmN, SSU98_1686
Organism
Streptococcus suis (strain 98HAH33)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs By similarity.UniRule annotation

Catalytic activityi

2 S-adenosyl-L-methionine + adenine(2503) in 23S rRNA = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine(2503) in 23S rRNA.UniRule annotation
2 S-adenosyl-L-methionine + adenine(37) in tRNA = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine(37) in tRNA.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei91 – 911Proton acceptor Reviewed prediction
Metal bindingi111 – 1111Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal bindingi115 – 1151Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal bindingi118 – 1181Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Binding sitei195 – 1951S-adenosyl-L-methionine By similarity
Binding sitei296 – 2961S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen By similarity
Active sitei340 – 3401S-methylcysteine intermediate By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW
  3. rRNA (adenine-C2-)-methyltransferase activity Source: UniProtKB-HAMAP
  4. rRNA binding Source: UniProtKB-HAMAP
  5. tRNA (adenine-C2-)-methyltransferase activity Source: UniProtKB-HAMAP
  6. tRNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. rRNA base methylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing, tRNA processing

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSSUI391296:GI2E-1742-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable dual-specificity RNA methyltransferase RlmN (EC:2.1.1.-, EC:2.1.1.192)
Alternative name(s):
23S rRNA (adenine(2503)-C(2))-methyltransferase
23S rRNA m2A2503 methyltransferase
Ribosomal RNA large subunit methyltransferase N
tRNA (adenine(37)-C(2))-methyltransferase
tRNA m2A37 methyltransferase
Gene namesi
Name:rlmN
Ordered Locus Names:SSU98_1686
OrganismiStreptococcus suis (strain 98HAH33)
Taxonomic identifieri391296 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000000244: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 370370Probable dual-specificity RNA methyltransferase RlmNUniRule annotation
PRO_0000350469Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi104 ↔ 340(transient) By similarity

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

STRINGi391296.SSU98_1686.

Structurei

3D structure databases

ProteinModelPortaliA4W3A5.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni163 – 1642S-adenosyl-L-methionine binding By similarity
Regioni218 – 2203S-adenosyl-L-methionine binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0820.
HOGENOMiHOG000217991.
KOiK06941.
OMAiHEGSKFE.
OrthoDBiEOG6DJZ2N.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01849. RNA_methyltr_RlmN.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR027492. RNA_MTrfase_RlmN.
IPR004383. rRNA_lsu_MTrfase_RlmN/Cfr.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR30544. PTHR30544. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF006004. CHP00048. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00048. TIGR00048. 1 hit.

Sequencei

Sequence statusi: Complete.

A4W3A5-1 [UniParc]FASTAAdd to Basket

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MKPSIYAFSQ ANLVDWILEN GEKKFRATQI WEWLYRSRVQ SFAEMTNLPK    50
SLIEKLEEHF VVNPLKQRIV QESKDGTIKY LFELPDGMLI ETVLMHQHYG 100
LSVCVTTQVG CNIGCTFCAS GLIPKQRDLT SGEIVAQIML VQKYLDERNQ 150
NERVSHIVVM GIGEPLDNYD NVMTFLRVVN DDKGLAIGAR HITVSTSGLA 200
PKIREFAREG VQVNLAVSLH APNNDLRSSI MRINRKFPIE VLFEAIEDYI 250
KVTNRRVTFE YIMLNEVNDG VEQAQELADL TKNIRKLSYI NLIPYNPVSE 300
HDQYSRSTKE RTLAFFDVLK KNGVNCVVRQ EHGTDIDAAC GQLRSNTLKK 350
DREKARARIA AAKAKAGIRA 370
Length:370
Mass (Da):42,060
Last modified:May 29, 2007 - v1
Checksum:iE90BAF008866B358
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000408 Genomic DNA. Translation: ABP92844.1.
RefSeqiYP_001201244.1. NC_009443.1.

Genome annotation databases

EnsemblBacteriaiABP92844; ABP92844; SSU98_1686.
GeneIDi5103113.
KEGGissv:SSU98_1686.
PATRICi19779594. VBIStrSui72275_1654.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000408 Genomic DNA. Translation: ABP92844.1 .
RefSeqi YP_001201244.1. NC_009443.1.

3D structure databases

ProteinModelPortali A4W3A5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 391296.SSU98_1686.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABP92844 ; ABP92844 ; SSU98_1686 .
GeneIDi 5103113.
KEGGi ssv:SSU98_1686.
PATRICi 19779594. VBIStrSui72275_1654.

Phylogenomic databases

eggNOGi COG0820.
HOGENOMi HOG000217991.
KOi K06941.
OMAi HEGSKFE.
OrthoDBi EOG6DJZ2N.

Enzyme and pathway databases

BioCyci SSUI391296:GI2E-1742-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01849. RNA_methyltr_RlmN.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR027492. RNA_MTrfase_RlmN.
IPR004383. rRNA_lsu_MTrfase_RlmN/Cfr.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR30544. PTHR30544. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF006004. CHP00048. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00048. TIGR00048. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "A glimpse of streptococcal toxic shock syndrome from comparative genomics of S. suis 2 Chinese isolates."
    Chen C., Tang J., Dong W., Wang C., Feng Y., Wang J., Zheng F., Pan X., Liu D., Li M., Song Y., Zhu X., Sun H., Feng T., Guo Z., Ju A., Ge J., Dong Y.
    , Sun W., Jiang Y., Wang J., Yan J., Yang H., Wang X., Gao G.F., Yang R., Wang J., Yu J.
    PLoS ONE 2:E315-E315(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 98HAH33.

Entry informationi

Entry nameiRLMN_STRS2
AccessioniPrimary (citable) accession number: A4W3A5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: May 29, 2007
Last modified: May 14, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue By similarity.

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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