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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Streptococcus suis (strain 98HAH33)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathway: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Hydroxyethylthiazole kinase (thiM)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei38 – 381Substrate; via amide nitrogenUniRule annotation
Binding sitei114 – 1141ATPUniRule annotation
Binding sitei159 – 1591ATPUniRule annotation
Binding sitei186 – 1861Substrate; via amide nitrogenUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSSUI391296:GI2E-783-MONOMER.
UniPathwayiUPA00060; UER00139.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:SSU98_0733
OrganismiStreptococcus suis (strain 98HAH33)
Taxonomic identifieri391296 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 236236Hydroxyethylthiazole kinasePRO_0000383908Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliA4W0K2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2145.
HOGENOMiHOG000114352.
KOiK00878.
OMAiLADEDHY.
OrthoDBiEOG628F8M.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.

Sequencei

Sequence statusi: Complete.

A4W0K2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYLEKLRSQN PLTICITNDV VKNFTANGLL ALGASPAMSE YPEDLEDLLP
60 70 80 90 100
YTKGLLINTG TLTNETWELY KSALDIAEKY GVPTVLDPVA AGAGAYRKKV
110 120 130 140 150
TLDLLHHHTI SLVRGNAGEI AALIGESIET KGVDSAQIDN VGELALRANQ
160 170 180 190 200
QLGIPVVITG KKDAVAVNHQ VRILENGSEL MPLVTGTGCL LGAVLAAFIH
210 220 230
LADEDHYWIV WKSPIGLFDR WRNGRAENLT TRNLPD
Length:236
Mass (Da):25,622
Last modified:May 29, 2007 - v1
Checksum:i0B2E9CDDA0CC771E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000408 Genomic DNA. Translation: ABP91891.1.
RefSeqiWP_011922790.1. NC_009443.1.

Genome annotation databases

EnsemblBacteriaiABP91891; ABP91891; SSU98_0733.
KEGGissv:SSU98_0733.
PATRICi19777705. VBIStrSui72275_0720.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000408 Genomic DNA. Translation: ABP91891.1.
RefSeqiWP_011922790.1. NC_009443.1.

3D structure databases

ProteinModelPortaliA4W0K2.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABP91891; ABP91891; SSU98_0733.
KEGGissv:SSU98_0733.
PATRICi19777705. VBIStrSui72275_0720.

Phylogenomic databases

eggNOGiCOG2145.
HOGENOMiHOG000114352.
KOiK00878.
OMAiLADEDHY.
OrthoDBiEOG628F8M.

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139.
BioCyciSSUI391296:GI2E-783-MONOMER.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "A glimpse of streptococcal toxic shock syndrome from comparative genomics of S. suis 2 Chinese isolates."
    Chen C., Tang J., Dong W., Wang C., Feng Y., Wang J., Zheng F., Pan X., Liu D., Li M., Song Y., Zhu X., Sun H., Feng T., Guo Z., Ju A., Ge J., Dong Y.
    , Sun W., Jiang Y., Wang J., Yan J., Yang H., Wang X., Gao G.F., Yang R., Wang J., Yu J.
    PLoS ONE 2:E315-E315(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 98HAH33.

Entry informationi

Entry nameiTHIM_STRS2
AccessioniPrimary (citable) accession number: A4W0K2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: May 29, 2007
Last modified: June 24, 2015
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.