A4VV12 (A4VV12_STRSY) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Ordered Locus Names:

SSU05_0985

Organism

Streptococcus suis (strain 05ZYH33) [Complete proteome] [HAMAP]

Taxonomic identifier

391295 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	713 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding RuleBase RU000518 SAAS SAAS005733 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS005733 RuleBase RU000518
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	A4VV12 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: C80A9A575C2DB0CA	FASTA	713	81,077
	10 20 30 40 50 60 MRFCCEVGDS GKVGEWLQDK LVRKKQQTKK NLVIVESPAK AKTIEKYLGK NYKVVASVGH 70 80 90 100 110 120 IRDLKKSSMS IDFDNNYAPE YINIRGKGPL INSLKKEAKN AKQVFLASDP DREGEAISWH 130 140 150 160 170 180 LAHILGLDAN DKNRVVFNEI TKDAVKNAFQ EPRAINHDLV DAQQARRVLD RIVGYSISPI 190 200 210 220 230 240 LWKKVKKGLS AGRVQSVALK LIIDRENEIS NFKPEEYWTI DATFKKGSKK FQASFYGIDG 250 260 270 280 290 300 KKTKIENNEQ VKEILARLDG DDFLVEQVER KERKRNAPLP YTTSTMQMDA ANKINFRTRK 310 320 330 340 350 360 TMMVAQQLYE GVSLGTGGTQ GLITYMRTDS TRISPVAQAQ AADFITERIG ATYSKHGNKV 370 380 390 400 410 420 QKCPVVHKMP TKPFVHQMFN LTPESIAKYL DKDQLRLYTL IRNRFVASQM AAAVFDTMSV 430 440 450 460 470 480 RLGQNGVIFA ANGSQVKFDG YMAVYNDSDK NKMLPDMEQG DTVVRTSTNP EQHFTQPPAR 490 500 510 520 530 540 YSEATLIKTL EENGVGRPST YAPTIETIQK RYYVKLAAKR FEPTELGEIV NKLIVEFFPD 550 560 570 580 590 600 IVNVKFTADM EQKLDDVEIG KEQWQKVIDG FYQPFKVELF KAEEQMEKIQ IKDEPAGFDC 610 620 630 640 650 660 DVCGHEMVIK LGRFGKFYAC SNFPDCRNTK QITKEIGVTC PVCQQGQVIE RKSKRNRLFY 670 680 690 700 710 GCDRYPECEF TSWDKPVGRA CPKCDHYLVE KKVRGGGKQV VCPNGDYEED KIK

References

[1]

"A glimpse of streptococcal toxic shock syndrome from comparative genomics of S. suis 2 Chinese isolates." BGI Chen C., Tang J., Dong W., Wang C., Feng Y., Wang J., Zheng F., Pan X., Liu D., Li M., Song Y., Zhu X., Sun H., Feng T., Guo Z., Ju A., Ge J., Dong Y. , Sun W., Jiang Y., Wang J., Yan J., Yang H., Wang X., Gao G.F., Yang R., Wang J., Yu J. PLoS ONE 2:E315-E315(2007) [PubMed: 17375201] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 05ZYH33.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000407 Genomic DNA. Translation: ABP89951.1.
RefSeq	YP_001198351.1. NC_009442.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4VV12.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTRT00000053283; EBSTRP00000049470; EBSTRG00000053273.
GeneID	5099304.
GenomeReviews	Gene locus SSU05_0985 in contig CP000407_GR.
KEGG	ssu:SSU05_0985.
PATRIC	19773743. VBIStrSui128929_0969.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0550.
GeneTree	EBGT00050000027862.
HOGENOM	HBG512935.
OMA	IERNIRH.
PhylomeDB	A4VV12.
ProtClustDB	PRK05582.
Enzyme and pathway databases
BioCyc	SSUI391295:SSU05_0985-MONOMER.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR013826. Topo_IA_cen_sub3. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 3 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 1 hit. G3DSA:1.10.290.10. Topo_IA_cen_sub3. 1 hit.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 2 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A4VV12_STRSY

Accession

Primary (citable) accession number: A4VV12

Entry history

Integrated into UniProtKB/TrEMBL:	May 29, 2007
Last sequence update:	May 29, 2007
Last modified:	December 14, 2011
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)