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A4VRK4

- TRMB_PSEU5

UniProt

A4VRK4 - TRMB_PSEU5

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Protein
tRNA (guanine-N(7)-)-methyltransferase
Gene
trmB, PST_3982
Organism
Pseudomonas stutzeri (strain A1501)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei68 – 681S-adenosyl-L-methionine By similarity
Binding sitei93 – 931S-adenosyl-L-methionine By similarity
Binding sitei120 – 1201S-adenosyl-L-methionine By similarity
Active sitei143 – 1431 By similarity
Binding sitei143 – 1431S-adenosyl-L-methionine By similarity
Binding sitei147 – 1471Substrate By similarity
Binding sitei179 – 1791Substrate By similarity

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciPSTU379731:GJER-3977-MONOMER.
    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferase
    tRNA(m7G46)-methyltransferase
    Gene namesi
    Name:trmB
    Ordered Locus Names:PST_3982
    OrganismiPseudomonas stutzeri (strain A1501)
    Taxonomic identifieri379731 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000000233: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 238238tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation
    PRO_1000064406Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi379731.PST_3982.

    Structurei

    3D structure databases

    ProteinModelPortaliA4VRK4.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni216 – 2194Substrate binding By similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000073968.
    KOiK03439.
    OMAiHSTLEMA.
    OrthoDBiEOG6K6VBC.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_01057. tRNA_methyltr_TrmB.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A4VRK4-1 [UniParc]FASTAAdd to Basket

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    MTEQNPAAEE QQSAPRRTIK SFVMRAGRMT EGQQRGLEQG WPKYGLELAD    50
    GLRDFDEVFG RSAPRTFEIG FGMGHSTLEM AAAAPEQDFI GVEVHKPGVG 100
    ALLSGLVSQK LTNVRVYSCD ALEVLRDCVA DASLDRVLLF FPDPWHKSRH 150
    HKRRIVQPAF AELVRRKLKV GGVLHMATDW EPYAEHMLEV MNVAPGYRNL 200
    AQDGRCVPRP TERPVTKFER RGERLGHGVW DLKFQRID 238
    Length:238
    Mass (Da):26,934
    Last modified:May 29, 2007 - v1
    Checksum:i9094E37DD6474AA1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000304 Genomic DNA. Translation: ABP81605.1.
    RefSeqiWP_011914987.1. NC_009434.1.
    YP_001174447.1. NC_009434.1.

    Genome annotation databases

    EnsemblBacteriaiABP81605; ABP81605; PST_3982.
    GeneIDi5096943.
    KEGGipsa:PST_3982.
    PATRICi19968468. VBIPseStu31643_3963.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000304 Genomic DNA. Translation: ABP81605.1 .
    RefSeqi WP_011914987.1. NC_009434.1.
    YP_001174447.1. NC_009434.1.

    3D structure databases

    ProteinModelPortali A4VRK4.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 379731.PST_3982.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABP81605 ; ABP81605 ; PST_3982 .
    GeneIDi 5096943.
    KEGGi psa:PST_3982.
    PATRICi 19968468. VBIPseStu31643_3963.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000073968.
    KOi K03439.
    OMAi HSTLEMA.
    OrthoDBi EOG6K6VBC.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .
    BioCyci PSTU379731:GJER-3977-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_01057. tRNA_methyltr_TrmB.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of the metabolically versatile and root-associated nitrogen-fixing bacterium Pseudomonas stutzeri A1501."
      Yan Y., Yang J., Dou Y., Ping S., Chen M., Yao Z., Li H., Lu W., Zhang W., Peng J., Liu W., He S., Geng L., Zhang X., Yang F., Li D., Lin Z., Wang Y.
      , Elmerich C., Lin M., Jin Q.
      Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: A1501.

    Entry informationi

    Entry nameiTRMB_PSEU5
    AccessioniPrimary (citable) accession number: A4VRK4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: May 29, 2007
    Last modified: September 3, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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