Reviewed, UniProtKB/Swiss-Prot A4VMU6 (STHA_PSEU5)

Names and origin

Protein names

Recommended name:
    Soluble pyridine nucleotide transhydrogenase
      Short name=STH
    EC=1.6.1.1
Alternative name(s):
    NAD(P)(+) transhydrogenase [B-specific]

Gene names

Name:	sthA
Ordered Locus Names:	PST_2647

Organism

Pseudomonas stutzeri (strain A1501) [Complete proteome] [HAMAP]

Taxonomic identifier

379731 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Conversion of NADPH, generated by peripheral catabolic pathways, to NADH, which can enter the respiratory chain for energy generation By similarity.
Catalytic activity	NADPH + NAD(+) = NADP(+) + NADH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NADP metabolic process Inferred from electronic annotation. Source: HAMAP cell redox homeostasis Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NAD(P)+ transhydrogenase (B-specific) activity Inferred from electronic annotation. Source: HAMAP electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	Soluble pyridine nucleotide transhydrogenase		PRO_1000012566
Regions
	Nucleotide binding	35 – 44	10	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4VMU6-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: CDBDFA44D311C6B8	FASTA	464	51,441
	10 20 30 40 50 60 MAVYNYDVVV LGSGPAGEGA AMNAVKAGRK VAVVDNRPLV GGNCTHLGTI PSKALRHSVR 70 80 90 100 110 120 QIMQYNTNPL FRQIGEPRWF SFPDVLKSAE KVISKQVTSR TSYYARNRID TFFGTASFSD 130 140 150 160 170 180 EQSVEVVCLN GMVEKLVANQ FVIATGSRPY RPADIDFNHP RIYDSDTILT LSHTPRRMII 190 200 210 220 230 240 YGAGVIGCEY ASIFSGLGVL VDLIDNREQL LSFLDDEISD ALSYHLRNNN VLIRHNEEYE 250 260 270 280 290 300 RVEGLENGVI LHLKSGKKIK ADAFLWSNGR TGNTDKLGLE NIGLKANSRG QVQVDEHYRT 310 320 330 340 350 360 EIGNIYAAGD VIGWPSLASA AYDQGRSAAG SIVENDSWRF VDDVPTGIYT IPEISSIGKN 370 380 390 400 410 420 ERELTQAKIP YEVGKAFFKS MARAQISFEP VGMLKILFHR ETLEVLGVHC FGYQASEIVH 430 440 450 460 IGQAIMNQKG PANSIKYFVN TTFNYPTMAE AYRVAAFDGL NRLF

References

[1]

"Complete genome sequence of the metabolically versatile and root-associated nitrogen-fixing bacterium Pseudomonas stutzeri A1501." Yan Y., Yang J., Dou Y., Ping S., Chen M., Yao Z., Li H., Lu W., Zhang W., Peng J., Liu W., He S., Geng L., Zhang X., Yang F., Li D., Lin Z., Wang Y. , Elmerich C., Lin M., Jin Q. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000304 Genomic DNA. Translation: ABP80297.1.
RefSeq	YP_001173139.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5095609.
GenomeReviews	Gene locus PST_2647 in contig CP000304_GR.
KEGG	psa:PST_2647.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00247. [Tree]
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR000815. Hg_reductase. IPR001100. Pyr_nuc-diS_OxRdtase. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR001327. Pyr_OxRdtase_NAD_bd. [Graphical view]
Gene3D	G3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF02852. Pyr_redox_dim. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00945. HGRDTASE. PR00411. PNDRDTASEI.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

STHA_PSEU5

Accession

Primary (citable) accession number: A4VMU6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 29, 2007
Last modified:	November 25, 2008
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)