A4VMC4 (ACEK_PSEU5) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 33.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Isocitrate dehydrogenase kinase/phosphatase Short name=IDH kinase/phosphatase Short name=IDHK/P EC=2.7.11.5 EC=3.1.3.- | ||||
| Gene names |
| ||||
| Organism | Pseudomonas stutzeri (strain A1501) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 379731 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas |
Protein attributes
| Sequence length | 573 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity. HAMAP MF_00747 |
| Catalytic activity | ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747 |
| Subcellular location | Cytoplasm By similarity HAMAP MF_00747. |
| Sequence similarities | Belongs to the AceK family. |
| Sequence caution | The sequence ABP80125.1 differs from that shown. Reason: Erroneous initiation. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Glyoxylate bypass Tricarboxylic acid cycle |
| Cellular component | Cytoplasm |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Hydrolase Kinase Protein phosphatase Transferase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycleInferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycleInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW [isocitrate dehydrogenase (NADP+)] kinase activityInferred from electronic annotation. Source: EC phosphoprotein phosphatase activityInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 573 | 573 | Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747 | PRO_0000315271 | |||||
Regions | |||||||||
| Nucleotide binding | 318 – 324 | 7 | ATP By similarity | ||||||
Sites | |||||||||
| Active site | 374 | 1 | By similarity | ||||||
| Binding site | 339 | 1 | ATP By similarity | ||||||
Sequences
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References
| [1] | "Complete genome sequence of the metabolically versatile and root-associated nitrogen-fixing bacterium Pseudomonas stutzeri A1501." Yan Y., Yang J., Dou Y., Ping S., Chen M., Yao Z., Li H., Lu W., Zhang W., Peng J., Liu W., He S., Geng L., Zhang X., Yang F., Li D., Lin Z., Wang Y. Jin Q.Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: A1501. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | CP000304 Genomic DNA. Translation: ABP80125.1. Different initiation. |
| RefSeq | YP_001172967.1. NC_009434.1. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | A4VMC4. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 5095366. |
| GenomeReviews | Gene locus PST_2473 in contig CP000304_GR. |
| KEGG | psa:PST_2473. |
| PATRIC | 19965444. VBIPseStu31643_2478. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| eggNOG | COG4579. |
| HOGENOM | HBG298593. |
| ProtClustDB | PRK02946. |
Enzyme and pathway databases | |
| BioCyc | PSTU379731:PST_2473-MONOMER. |
Family and domain databases | |
| HAMAP | MF_00747. AceK. [Tree] |
| InterPro | IPR010452. Isocitrate_DH_AceK. [Graphical view] |
| KO | K00906. |
| Pfam | PF06315. AceK. 1 hit. [Graphical view] |
| PIRSF | PIRSF000719. AceK. 1 hit. |
| ProDom | PD043552. Isocitrate_DH_AceK. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| ProtoNet | Search... |
Entry information
| Entry name | ACEK_PSEU5 | ||||||||
| Accession | Primary (citable) accession number: A4VMC4 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |

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