A4VMC4 (ACEK_PSEU5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isocitrate dehydrogenase kinase/phosphatase
Short name=IDH kinase/phosphatase
Short name=IDHK/P
EC=2.7.11.5
EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	PST_2473

Organism

Pseudomonas stutzeri (strain A1501) [Complete proteome] [HAMAP]

Taxonomic identifier

379731 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	573 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity. HAMAP MF_00747
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity HAMAP MF_00747.
Sequence similarities	Belongs to the AceK family.
Sequence caution	The sequence ABP80125.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: EC phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 573	573	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_0000315271
Regions
	Nucleotide binding	318 – 324	7	ATP By similarity
Sites
	Active site	374	1	By similarity
	Binding site	339	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4VMC4 [UniParc]. Last modified January 15, 2008. Version 2. Checksum: C03135DC9CC7FB71	FASTA	573	66,310
	10 20 30 40 50 60 MVQHELAAEI ARQILLGFDD YREHFRLITE GARARFEQAQ WQEAQRASAA RISLYEEKVG 70 80 90 100 110 120 ETRRRLLASF DHADLLQVEA WPQIKSAYIE LINPRFDDEL SETWYNSIFC GLFSHDCISD 130 140 150 160 170 180 GTMFIHTTRP AVRAHERLAQ TRRYRPNGDL RGMLEQILRD YAFSVPYCDL PGDLQRLQTQ 190 200 210 220 230 240 LCETLPDWVC KDPELTVEVF VSVLYRNKGA YLIGRIFTCD EQWPLAIPLL HRDGQGIVAD 250 260 270 280 290 300 ALITDEAEVS IIFSFTRSYF MVEVPIPAEF VGFLKRILPG KHIAELYSSI GFYKHGKSEF 310 320 330 340 350 360 YRALIDHLAN TDDRFVMAPG VRGMVMTVFT LPGFNTVFKL IKDRFSPIKS VNRANVIEKY 370 380 390 400 410 420 RLVKSVDRVG RMADTQEFAD FRFPKAKFEP ECLAELLEVA PSTVEVQGDT VLVRHCWTER 430 440 450 460 470 480 RMTPLNLYLE NASEQQVREA LEDYGLAIKQ LAAANIFPGD MLLKNFGVTR HGRVVFYDYD 490 500 510 520 530 540 EISFLTEVNF RHIPPPRYPE DEMASEPWYS VGPNDVFPEE FPRFLFADLG QRRLFASLHG 550 560 570 ELYDADYWKG LQDAIRQGKV IDVFPYRRKA DRN

References

[1]

"Complete genome sequence of the metabolically versatile and root-associated nitrogen-fixing bacterium Pseudomonas stutzeri A1501." Yan Y., Yang J., Dou Y., Ping S., Chen M., Yao Z., Li H., Lu W., Zhang W., Peng J., Liu W., He S., Geng L., Zhang X., Yang F., Li D., Lin Z., Wang Y. , Elmerich C., Lin M., Jin Q. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: A1501.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000304 Genomic DNA. Translation: ABP80125.1. Different initiation.
RefSeq	YP_001172967.1. NC_009434.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4VMC4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5095366.
GenomeReviews	Gene locus PST_2473 in contig CP000304_GR.
KEGG	psa:PST_2473.
PATRIC	19965444. VBIPseStu31643_2478.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG4579.
HOGENOM	HBG298593.
ProtClustDB	PRK02946.
Enzyme and pathway databases
BioCyc	PSTU379731:PST_2473-MONOMER.
Family and domain databases
HAMAP	MF_00747. AceK. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
KO	K00906.
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProDom	PD043552. Isocitrate_DH_AceK. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

ACEK_PSEU5

Accession

Primary (citable) accession number: A4VMC4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 15, 2008
Last modified:	January 25, 2012
This is version 33 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program