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Protein

Acireductone dioxygenase

Gene

mtnD

Organism
Pseudomonas stutzeri (strain A1501)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes 2 different reactions between oxygene and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway. Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway.UniRule annotation

Catalytic activityi

1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 = 3-(methylthio)propanoate + formate + CO.UniRule annotation
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 = 4-(methylthio)-2-oxobutanoate + formate.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Fe cationUniRule annotationNote: Binds 1 Fe cation per monomer.UniRule annotation
  • Ni2+UniRule annotationNote: Binds 1 nickel ion per monomer.UniRule annotation

Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 5 of the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Methylthioribose-1-phosphate isomerase (mtnA)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. Acireductone dioxygenase (mtnD)
  6. no protein annotated in this organism
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate, the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei96 – 961May play a role in metal incorporation in vivoUniRule annotation
Metal bindingi97 – 971Iron; alternateUniRule annotation
Metal bindingi97 – 971Nickel; alternateUniRule annotation
Metal bindingi99 – 991Iron; alternateUniRule annotation
Metal bindingi99 – 991Nickel; alternateUniRule annotation
Sitei102 – 1021May play a role in transmitting local conformational changesUniRule annotation
Metal bindingi103 – 1031Iron; alternateUniRule annotation
Metal bindingi103 – 1031Nickel; alternateUniRule annotation
Sitei105 – 1051Important to generate the dianionUniRule annotation
Metal bindingi141 – 1411Iron; alternateUniRule annotation
Metal bindingi141 – 1411Nickel; alternateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Iron, Metal-binding, Nickel

Enzyme and pathway databases

BioCyciPSTU379731:GJER-2427-MONOMER.
UniPathwayiUPA00904; UER00878.

Names & Taxonomyi

Protein namesi
Recommended name:
Acireductone dioxygenaseUniRule annotation
Alternative name(s):
1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenaseUniRule annotation
Short name:
DHK-MTPene dioxygenaseUniRule annotation
Acireductone dioxygenase (Fe(2+)-requiring)UniRule annotation (EC:1.13.11.54UniRule annotation)
Short name:
ARD'UniRule annotation
Short name:
Fe-ARDUniRule annotation
Acireductone dioxygenase (Ni(2+)-requiring)UniRule annotation (EC:1.13.11.53UniRule annotation)
Short name:
ARDUniRule annotation
Short name:
Ni-ARDUniRule annotation
Gene namesi
Name:mtnDUniRule annotation
Ordered Locus Names:PST_2431
OrganismiPseudomonas stutzeri (strain A1501)
Taxonomic identifieri379731 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000000233 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 181181Acireductone dioxygenasePRO_0000359222Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi379731.PST_2431.

Structurei

3D structure databases

ProteinModelPortaliA4VM82.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the acireductone dioxygenase (ARD) family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108CAF. Bacteria.
COG1791. LUCA.
HOGENOMiHOG000201072.
KOiK08967.
OMAiWFDMGSE.
OrthoDBiPOG091H0K83.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
HAMAPiMF_01682. Salvage_MtnD. 1 hit.
InterProiIPR004313. ARD.
IPR023956. ARD_bac.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR23418. PTHR23418. 1 hit.
PfamiPF03079. ARD. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.

Sequencei

Sequence statusi: Complete.

A4VM82-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVLSVYHES RPEQPLKVLT HLEDIAATLA EVGVQLERWD ASAPVAAGAS
60 70 80 90 100
QEDVIAAYRP QIDRLMAERG YVTVDVISVS RDHPQKDELR AKFLDEHRHA
110 120 130 140 150
EDEVRFFVAG RGLFTLHIED MVYAVLCEKN DLISVPAGTR HWFDMGEQPS
160 170 180
FVAIRLFNNP DGWTAQFTGE RIADQFPRLD D
Length:181
Mass (Da):20,504
Last modified:May 29, 2007 - v1
Checksum:i1C44A0B1FFBC59BD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000304 Genomic DNA. Translation: ABP80083.1.
RefSeqiWP_011913546.1. NC_009434.1.

Genome annotation databases

EnsemblBacteriaiABP80083; ABP80083; PST_2431.
GeneIDi25042325.
KEGGipsa:PST_2431.
PATRICi19965356. VBIPseStu31643_2434.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000304 Genomic DNA. Translation: ABP80083.1.
RefSeqiWP_011913546.1. NC_009434.1.

3D structure databases

ProteinModelPortaliA4VM82.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi379731.PST_2431.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABP80083; ABP80083; PST_2431.
GeneIDi25042325.
KEGGipsa:PST_2431.
PATRICi19965356. VBIPseStu31643_2434.

Phylogenomic databases

eggNOGiENOG4108CAF. Bacteria.
COG1791. LUCA.
HOGENOMiHOG000201072.
KOiK08967.
OMAiWFDMGSE.
OrthoDBiPOG091H0K83.

Enzyme and pathway databases

UniPathwayiUPA00904; UER00878.
BioCyciPSTU379731:GJER-2427-MONOMER.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
HAMAPiMF_01682. Salvage_MtnD. 1 hit.
InterProiIPR004313. ARD.
IPR023956. ARD_bac.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR23418. PTHR23418. 1 hit.
PfamiPF03079. ARD. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMTND_PSEU5
AccessioniPrimary (citable) accession number: A4VM82
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: May 29, 2007
Last modified: September 7, 2016
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.