A4VL72 (SYQ_PSEU5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamine--tRNA ligase
EC=6.1.1.18

Alternative name(s):
Glutaminyl-tRNA synthetase
Short name=GlnRS

Gene names

Name:	glnS
Ordered Locus Names:	PST_2052

Organism

Pseudomonas stutzeri (strain A1501) [Complete proteome] [HAMAP]

Taxonomic identifier

379731 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	555 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP MF_00126
Subunit structure	Monomer By similarity. HAMAP MF_00126
Subcellular location	Cytoplasm By similarity HAMAP MF_00126.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 555	555	Glutamine--tRNA ligase HAMAP MF_00126		PRO_1000095503
Regions
	Motif	35 – 45	11	"HIGH" region HAMAP MF_00126
	Motif	269 – 273	5	"KMSKS" region HAMAP MF_00126
Sites
	Binding site	272	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4VL72 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 7032B6A907054549	FASTA	555	62,959
	10 20 30 40 50 60 MSKPETPAAS NFLRPIVQAD LDSGKHAKII TRFPPEPNGY LHIGHAKSIC LNFGLAKEFG 70 80 90 100 110 120 GECNLRFDDT NPAKEDQEYI DAIKSDVQWL GFQWAGEERY ASDYFDQLHD WAVHLIKAGK 130 140 150 160 170 180 AYVCDLTPEQ AREYRGSLTE PGTNSPFRER SVEENLDLFA RMKAGEFPDG ARALRAKIDM 190 200 210 220 230 240 ASPNMNLRDP ILYRIRHAHH HQTGDKWCIY PSYDFTHGQS DAIEGITHSI CTLEFEDHRP 250 260 270 280 290 300 LYEWFLANLP VPAQPRQYEF ARLNLNYTIT SKRKLKQLVD EKHVSGWDDP RMSTLSGFRR 310 320 330 340 350 360 RGYTPASIRN FCDMIGVNRA GGVVDIGMLE FAIREDLDAN AARAMCVLKP LKVVITNYPQ 370 380 390 400 410 420 DQVENLELPR HPKQDMGVRV LPFSREIYID AGDFEEVPPA GFKRLVPGGE VRLRGSYVIR 430 440 450 460 470 480 ADEAVKDDQG NIVELRCSYD ENTLGKNPEG RKVKGVIHWV PAAESVECEV RLYDRLFRSA 490 500 510 520 530 540 NPEKDEEGGS FLDNINPESL VVLKGCRAEP SLANAAPEER FQFEREGYFC ADMKDSKPGA 550 PVFNRTVTLR DSWGQ

References

[1]

"Complete genome sequence of the metabolically versatile and root-associated nitrogen-fixing bacterium Pseudomonas stutzeri A1501." Yan Y., Yang J., Dou Y., Ping S., Chen M., Yao Z., Li H., Lu W., Zhang W., Peng J., Liu W., He S., Geng L., Zhang X., Yang F., Li D., Lin Z., Wang Y. , Elmerich C., Lin M., Jin Q. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: A1501.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000304 Genomic DNA. Translation: ABP79723.1.
RefSeq	YP_001172565.1. NC_009434.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4VL72.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5098135.
GenomeReviews	Gene locus PST_2052 in contig CP000304_GR.
KEGG	psa:PST_2052.
PATRIC	19964601. VBIPseStu31643_2070.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG334108.
OMA	RMPTIAG.
PhylomeDB	A4VL72.
ProtClustDB	PRK05347.
Enzyme and pathway databases
BioCyc	PSTU379731:PST_2052-MONOMER.
Family and domain databases
HAMAP	MF_00126. Gln_tRNA_synth. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth_Ib. IPR022861. Gln_tRNA_synth_bac. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. IPR011035. Ribosomal_L25/Gln-tRNA_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:2.40.240.10. Rbsml_L25/Gln-tRNA_synth_b-brl. 2 hits. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01886.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF50715. Ribosomal_L25rel. 1 hit.
TIGRFAMs	TIGR00440. GlnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYQ_PSEU5

Accession

Primary (citable) accession number: A4VL72

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	May 29, 2007
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program