ID   FADB_PSEU5              Reviewed;         715 AA.
AC   A4VKA3;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   29-MAY-2007, sequence version 1.
DT   16-JUN-2009, entry version 17.
DE   RecName: Full=Fatty acid oxidation complex subunit alpha;
DE   Includes:
DE     RecName: Full=Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase;
DE              EC=4.2.1.17;
DE              EC=5.3.3.8;
DE              EC=5.1.2.3;
DE   Includes:
DE     RecName: Full=3-hydroxyacyl-CoA dehydrogenase;
DE              EC=1.1.1.35;
GN   Name=fadB; OrderedLocusNames=PST_1726;
OS   Pseudomonas stutzeri (strain A1501).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=379731;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Yan Y., Yang J., Dou Y., Ping S., Chen M., Yao Z., Li H., Lu W.,
RA   Zhang W., Peng J., Liu W., He S., Geng L., Zhang X., Yang F., Li D.,
RA   Lin Z., Wang Y., Elmerich C., Lin M., Jin Q.;
RT   "Complete genome sequence of the metabolically versatile and root-
RT   associated nitrogen-fixing bacterium Pseudomonas stutzeri A1501.";
RL   Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the formation of an hydroxyacyl-CoA by
CC       addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA
CC       epimerase and 3-hydroxyacyl-CoA dehydrogenase activities (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: (S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA
CC       + NADH.
CC   -!- CATALYTIC ACTIVITY: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-
CC       CoA + H(2)O.
CC   -!- CATALYTIC ACTIVITY: (S)-3-hydroxybutanoyl-CoA = (R)-3-
CC       hydroxybutanoyl-CoA.
CC   -!- CATALYTIC ACTIVITY: (3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-
CC       CoA.
CC   -!- PATHWAY: Lipid metabolism; fatty acid beta-oxidation.
CC   -!- SUBUNIT: Heterotetramer of two alpha chains (fadB) and two beta
CC       chains (fadA) (By similarity).
CC   -!- SIMILARITY: In the N-terminal section; belongs to the enoyl-CoA
CC       hydratase/isomerase family.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the 3-
CC       hydroxyacyl-CoA dehydrogenase family.
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DR   EMBL; CP000304; ABP79404.1; -; Genomic_DNA.
DR   RefSeq; YP_001172246.1; -.
DR   GeneID; 5097810; -.
DR   GenomeReviews; CP000304_GR; PST_1726.
DR   KEGG; psa:PST_1726; -.
DR   OMA; A4VKA3; QFANSIF.
DR   GO; GO:0016507; C:fatty acid beta-oxidation multienzyme complex; IEA:InterPro.
DR   GO; GO:0003857; F:3-hydroxyacyl-CoA dehydrogenase activity; IEA:HAMAP.
DR   GO; GO:0008692; F:3-hydroxybutyryl-CoA epimerase activity; IEA:HAMAP.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0004165; F:dodecenoyl-CoA delta-isomerase activity; IEA:HAMAP.
DR   GO; GO:0004300; F:enoyl-CoA hydratase activity; IEA:HAMAP.
DR   GO; GO:0009062; P:fatty acid catabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_01621; -; 1.
DR   InterPro; IPR006180; 3-OHacyl-CoA_DH_CS.
DR   InterPro; IPR006176; 3-OHacyl-CoA_DH_NAD-bd.
DR   InterPro; IPR006108; 3HC_DH_C.
DR   InterPro; IPR001753; Crotonase_core.
DR   InterPro; IPR018376; Enoyl-CoA_hyd/isom_CS.
DR   InterPro; IPR012799; FadB.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF00725; 3HCDH; 1.
DR   Pfam; PF02737; 3HCDH_N; 1.
DR   Pfam; PF00378; ECH; 1.
DR   TIGRFAMs; TIGR02437; FadB; 1.
DR   PROSITE; PS00067; 3HCDH; 1.
DR   PROSITE; PS00166; ENOYL_COA_HYDRATASE; FALSE_NEG.
PE   3: Inferred from homology;
KW   Complete proteome; Fatty acid metabolism; Isomerase;
KW   Lipid degradation; Lipid metabolism; Lyase; Multifunctional enzyme;
KW   NAD; Oxidoreductase.
FT   CHAIN         1    715       Fatty acid oxidation complex subunit
FT                                alpha.
FT                                /FTId=PRO_1000069570.
FT   REGION        1    190       Enoyl-CoA hydratase/isomerase (By
FT                                similarity).
FT   REGION      312    715       3-hydroxyacyl-CoA dehydrogenase (By
FT                                similarity).
SQ   SEQUENCE   715 AA;  77618 MW;  BDEE81C81BE38515 CRC64;
     MIYEGKAITV KALESGIVEL NFDLKGESVN KFNRLTLNDL RQAVDAIKAD ASVKGVIVTS
     GKDVFIVGAD ITEFVDNFKL PDEELVAGNL EANKIFSDFE DLGVPTVVAI NGIALGGGFE
     MCMAADYRVM STTAKVGLPE VKLGIYPGFG GTVRLPRLIG VDNAVEWIAS GKENRAEDAL
     KVHAVDAVVA PEKLQEAALD LVKRAISGEL DYKAKRQPKL DKLKLNAIEQ MMAFETSKAF
     VAGQAGPNYP APVEAIKTIQ KAANFGRDKA IEVEAAGFVK LAKTSVAQSL VGLFLSDQEL
     KKKAKAYDKQ ARDVKLAAVL GAGIMGGGIA YQSAVKGTPI LMKDIREEGI QMGLDEASKL
     LGKRVEKGRL TPEKMAQALN AIRPTMSYGD FGNVDIVVEA VVENPKVKHA VLAEVEGHVR
     EDAIIASNTS TISISYLAQA LKRPENFCGM HFFNPVHMMP LVEVIRGEKT SETAIATTVA
     YAKKMGKSPV VVNDCPGFLV NRVLFPYFGG FARAIAHGVD FVRADKVMEK FGWPMGPAYL
     MDVVGMDTGH HGRDVMAEGF PDRMKDDTRT AVDVMYDANR LGQKNGKGFY AYEMDKKGKP
     KKVVDPQAYE LLKPIVAETR ELSDEDIINY MMIPLCLETV RCLEDGIVET AAEADMGLIY
     GIGFPPFRGG ALRYIDSIGV AEFVAMADKY ADLGPLYHPT AKLREMAANG QRFYG
//