SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

A4VJR9

- GLND_PSEU5

UniProt

A4VJR9 - GLND_PSEU5

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene
glnD, PST_1535
Organism
Pseudomonas stutzeri (strain A1501)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen fixation and metabolism By similarity.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. metal ion binding Source: InterPro
  4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen fixation Source: UniProtKB-HAMAP
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciPSTU379731:GJER-1534-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Short name:
UTase/UR
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein
Including the following 2 domains:
[Protein-PII] uridylyltransferase (EC:2.7.7.59)
Short name:
PII uridylyltransferase
Short name:
UTase
[Protein-PII]-UMP uridylyl-removing enzyme (EC:3.1.4.-)
Short name:
UR
Gene namesi
Name:glnD
Ordered Locus Names:PST_1535
OrganismiPseudomonas stutzeri (strain A1501)
Taxonomic identifieri379731 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000000233: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 900900Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotationPRO_1000078809Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi379731.PST_1535.

Structurei

3D structure databases

ProteinModelPortaliA4VJR9.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini462 – 590129HDAdd
BLAST
Domaini706 – 78984ACT 1Add
BLAST
Domaini816 – 89681ACT 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 342342UridylyltransferaseUniRule annotationAdd
BLAST
Regioni343 – 705363Uridylyl-removingUniRule annotationAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing By similarity.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.
Contains 2 ACT domains.
Contains 1 HD domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4VJR9-1 [UniParc]FASTAAdd to Basket

« Hide

MPQVDPELFD RSQFQAELAL KASPIAAYKK AIRQARQVLD ERFRAGRDIR    50
RLIEDRAWFV DQILRSAWSR FDWDKGADIA LVAVGGYGRG ELHPYSDIDL 100
LILLDENDQE IFREPIEGFL TLLWDIGLEV GQAVRSVAEC ADEARADLTV 150
ITNLMESRTI AGPERLRQAM LQVTSTQQMW PSKEFFLAKR NEQRARHAKY 200
NNTEYNLEPN VKGSPGGLRD IQTILWIARR EFGTLNLQAM VDQGFITEGE 250
HSLLTAAQEF LWKVRYGLHM LAGRAEDRLL FDHQRSLAAL LGYEDSDAKL 300
AIERFMQKYY RVVMSIAELS DLVGQHFAEV ILWEGESGPI VPLNSRFQVR 350
DGYLEVSNPA IFKRTPFAIL ETFVLLAQHP DIQGVRSDTI RLLRDHRYLI 400
DDTFRQDLRN TSLFIELFKC KEGIHRNLRR MNRYGILGRY LPEFGHIVGQ 450
MQHDLFHIYT VDAHTLNVIK YLRKLTKPGV AEKYPLASKL VEKLPKPELI 500
YIAGLYHDIA KGRGGDHSEL GAVDAEQFCR RHKLPAWDTR LVVWLVENHL 550
VMSTTAQRKD LSDPQVINDF AQRVGDETHL DYLYVLTVAD INATNPTLWN 600
SWRASLLRQL YTETKRALKR GLENPLGREE QIRQTQRAAL DDLVRHGTDP 650
DDAEQLWAQL GDDYFLRHTA TDVAWHTDAI IEHPANGGPL VLIKETTQRE 700
FEGGTQIFIY APDQHDFFAV TVAAMDQLNL NIHDARILTS SSQFTLDTYI 750
VLEADGSPIG NNPERIEEIR SGLIAALRNP DDYLTIIQRR VPRQLKHFAF 800
PPQVTIHNDT QRPQTILEII APDRPGLLAR VGQLFLDFDL SVQNAKIATL 850
GERVEDVFFV TDADNQPLSD PQLCLRLQQA IIKELQQENE QQPSPSSIVI 900
Length:900
Mass (Da):102,974
Last modified:May 29, 2007 - v1
Checksum:i71172F92D9FFEF11
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000304 Genomic DNA. Translation: ABP79220.1.
RefSeqiYP_001172062.1. NC_009434.1.

Genome annotation databases

EnsemblBacteriaiABP79220; ABP79220; PST_1535.
GeneIDi5097620.
KEGGipsa:PST_1535.
PATRICi19963513. VBIPseStu31643_1536.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000304 Genomic DNA. Translation: ABP79220.1 .
RefSeqi YP_001172062.1. NC_009434.1.

3D structure databases

ProteinModelPortali A4VJR9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 379731.PST_1535.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABP79220 ; ABP79220 ; PST_1535 .
GeneIDi 5097620.
KEGGi psa:PST_1535.
PATRICi 19963513. VBIPseStu31643_1536.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261778.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci PSTU379731:GJER-1534-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of the metabolically versatile and root-associated nitrogen-fixing bacterium Pseudomonas stutzeri A1501."
    Yan Y., Yang J., Dou Y., Ping S., Chen M., Yao Z., Li H., Lu W., Zhang W., Peng J., Liu W., He S., Geng L., Zhang X., Yang F., Li D., Lin Z., Wang Y.
    , Elmerich C., Lin M., Jin Q.
    Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: A1501.

Entry informationi

Entry nameiGLND_PSEU5
AccessioniPrimary (citable) accession number: A4VJR9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 29, 2007
Last modified: June 11, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi