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A4VJR9

- GLND_PSEU5

UniProt

A4VJR9 - GLND_PSEU5

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Pseudomonas stutzeri (strain A1501)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen fixation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen fixation Source: UniProtKB-HAMAP
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciPSTU379731:GJER-1534-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:PST_1535
OrganismiPseudomonas stutzeri (strain A1501)
Taxonomic identifieri379731 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000000233: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 900900Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_1000078809Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi379731.PST_1535.

Structurei

3D structure databases

ProteinModelPortaliA4VJR9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini462 – 590129HDUniRule annotationAdd
BLAST
Domaini706 – 78984ACT 1UniRule annotationAdd
BLAST
Domaini816 – 89681ACT 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 342342UridylyltransferaseAdd
BLAST
Regioni343 – 705363Uridylyl-removingAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation
Contains 2 ACT domains.UniRule annotation
Contains 1 HD domain.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4VJR9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPQVDPELFD RSQFQAELAL KASPIAAYKK AIRQARQVLD ERFRAGRDIR
60 70 80 90 100
RLIEDRAWFV DQILRSAWSR FDWDKGADIA LVAVGGYGRG ELHPYSDIDL
110 120 130 140 150
LILLDENDQE IFREPIEGFL TLLWDIGLEV GQAVRSVAEC ADEARADLTV
160 170 180 190 200
ITNLMESRTI AGPERLRQAM LQVTSTQQMW PSKEFFLAKR NEQRARHAKY
210 220 230 240 250
NNTEYNLEPN VKGSPGGLRD IQTILWIARR EFGTLNLQAM VDQGFITEGE
260 270 280 290 300
HSLLTAAQEF LWKVRYGLHM LAGRAEDRLL FDHQRSLAAL LGYEDSDAKL
310 320 330 340 350
AIERFMQKYY RVVMSIAELS DLVGQHFAEV ILWEGESGPI VPLNSRFQVR
360 370 380 390 400
DGYLEVSNPA IFKRTPFAIL ETFVLLAQHP DIQGVRSDTI RLLRDHRYLI
410 420 430 440 450
DDTFRQDLRN TSLFIELFKC KEGIHRNLRR MNRYGILGRY LPEFGHIVGQ
460 470 480 490 500
MQHDLFHIYT VDAHTLNVIK YLRKLTKPGV AEKYPLASKL VEKLPKPELI
510 520 530 540 550
YIAGLYHDIA KGRGGDHSEL GAVDAEQFCR RHKLPAWDTR LVVWLVENHL
560 570 580 590 600
VMSTTAQRKD LSDPQVINDF AQRVGDETHL DYLYVLTVAD INATNPTLWN
610 620 630 640 650
SWRASLLRQL YTETKRALKR GLENPLGREE QIRQTQRAAL DDLVRHGTDP
660 670 680 690 700
DDAEQLWAQL GDDYFLRHTA TDVAWHTDAI IEHPANGGPL VLIKETTQRE
710 720 730 740 750
FEGGTQIFIY APDQHDFFAV TVAAMDQLNL NIHDARILTS SSQFTLDTYI
760 770 780 790 800
VLEADGSPIG NNPERIEEIR SGLIAALRNP DDYLTIIQRR VPRQLKHFAF
810 820 830 840 850
PPQVTIHNDT QRPQTILEII APDRPGLLAR VGQLFLDFDL SVQNAKIATL
860 870 880 890 900
GERVEDVFFV TDADNQPLSD PQLCLRLQQA IIKELQQENE QQPSPSSIVI
Length:900
Mass (Da):102,974
Last modified:May 29, 2007 - v1
Checksum:i71172F92D9FFEF11
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000304 Genomic DNA. Translation: ABP79220.1.
RefSeqiYP_001172062.1. NC_009434.1.

Genome annotation databases

EnsemblBacteriaiABP79220; ABP79220; PST_1535.
GeneIDi5097620.
KEGGipsa:PST_1535.
PATRICi19963513. VBIPseStu31643_1536.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000304 Genomic DNA. Translation: ABP79220.1 .
RefSeqi YP_001172062.1. NC_009434.1.

3D structure databases

ProteinModelPortali A4VJR9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 379731.PST_1535.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABP79220 ; ABP79220 ; PST_1535 .
GeneIDi 5097620.
KEGGi psa:PST_1535.
PATRICi 19963513. VBIPseStu31643_1536.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261778.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci PSTU379731:GJER-1534-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of the metabolically versatile and root-associated nitrogen-fixing bacterium Pseudomonas stutzeri A1501."
    Yan Y., Yang J., Dou Y., Ping S., Chen M., Yao Z., Li H., Lu W., Zhang W., Peng J., Liu W., He S., Geng L., Zhang X., Yang F., Li D., Lin Z., Wang Y.
    , Elmerich C., Lin M., Jin Q.
    Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: A1501.

Entry informationi

Entry nameiGLND_PSEU5
AccessioniPrimary (citable) accession number: A4VJR9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 29, 2007
Last modified: November 26, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3