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A4VII0 (DDL_PSEU5) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:PST_1084
OrganismPseudomonas stutzeri (strain A1501) [Complete proteome] [HAMAP]
Taxonomic identifier379731 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length314 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 314314D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000341156

Regions

Domain112 – 307196ATP-grasp
Nucleotide binding138 – 19356ATP By similarity

Sites

Metal binding2611Magnesium or manganese 1 By similarity
Metal binding2741Magnesium or manganese 1 By similarity
Metal binding2741Magnesium or manganese 2 By similarity
Metal binding2761Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A4VII0 [UniParc].

Last modified May 29, 2007. Version 1.
Checksum: 1436C0AE352581AD

FASTA31433,316
        10         20         30         40         50         60 
MMALQSTRDP KEFGRVAVLF GGKSAEREVS LKSGAAVLAA LQAAGVDAFG IDAGDDLLQR 

        70         80         90        100        110        120 
LSSERIDRAF IVLHGRGGED GSMQGLLECA GIPYTGSGIL ASALAMDKLR TKQVWQSLGL 

       130        140        150        160        170        180 
PTPRHAVLAS EVDCQAAAQM LGFPLIVKPA HEGSSIGMAK VGDVAELIAA WRAASAYDAQ 

       190        200        210        220        230        240 
VLVEQWIQGP EFTVAVLHGE VLPPIGLGTP HTFYDYDAKY LASDTQYRIP CGLDAAREEA 

       250        260        270        280        290        300 
LKVLSARACE AVGIRGWARV DVMQDAEGNF WLLEVNTVPG MTDHSLVPMA ARAAGLDFQQ 

       310 
LVLSILDDSL EAGN 

« Hide

References

[1]"Complete genome sequence of the metabolically versatile and root-associated nitrogen-fixing bacterium Pseudomonas stutzeri A1501."
Yan Y., Yang J., Dou Y., Ping S., Chen M., Yao Z., Li H., Lu W., Zhang W., Peng J., Liu W., He S., Geng L., Zhang X., Yang F., Li D., Lin Z., Wang Y. expand/collapse author list , Elmerich C., Lin M., Jin Q.
Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: A1501.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000304 Genomic DNA. Translation: ABP78781.1.
RefSeqYP_001171623.1. NC_009434.1.

3D structure databases

ProteinModelPortalA4VII0.
SMRA4VII0. Positions 14-307.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING379731.PST_1084.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABP78781; ABP78781; PST_1084.
GeneID5098754.
KEGGpsa:PST_1084.
PATRIC19962587. VBIPseStu31643_1080.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMADTQYRIP.
OrthoDBEOG6ND0KB.
ProtClustDBPRK01372.

Enzyme and pathway databases

BioCycPSTU379731:GJER-1085-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_PSEU5
AccessionPrimary (citable) accession number: A4VII0
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: May 29, 2007
Last modified: February 19, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways