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A4VI60

- SYI_PSEU5

UniProt

A4VI60 - SYI_PSEU5

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Pseudomonas stutzeri (strain A1501)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 55 (01 Oct 2014)
      Sequence version 1 (29 May 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei567 – 5671Aminoacyl-adenylateUniRule annotation
    Binding sitei611 – 6111ATPUniRule annotation
    Metal bindingi906 – 9061ZincUniRule annotation
    Metal bindingi909 – 9091ZincUniRule annotation
    Metal bindingi926 – 9261ZincUniRule annotation
    Metal bindingi929 – 9291ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciPSTU379731:GJER-965-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:PST_0964
    OrganismiPseudomonas stutzeri (strain A1501)
    Taxonomic identifieri379731 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000000233: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 943943Isoleucine--tRNA ligasePRO_1000022109Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi379731.PST_0964.

    Structurei

    3D structure databases

    ProteinModelPortaliA4VI60.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi608 – 6125"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A4VI60-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTDYKATLNL PSTAFPMKAG LPQREPEILQ RWNSIDLYRK LRQIGEGRPK    50
    FILHDGPPYA NGNIHIGHAV NKVIKDMIVR SRTLAGFDAP YVPGWDCHGL 100
    PIEHKVETTF GKNQPADLTR ERCRAYAAEQ IEGQKADFIR LGVLGDWDNP 150
    YKTMDFANEA GEIRALAKMV EGGFVFKGLK PVNWCFDCGS ALAEAEVEYQ 200
    DKKSDAIDVA FDVEDADKLA AAFGLASLAK RASIVIWTTT PWTIPANQAL 250
    NVHPEFVYAL VDTGERLLVL AEELVESCLQ RYGLAGEIIA RCEGKALELI 300
    RFRHPFYERF APVYLADYVE TGAGTGIVHS APAYGEDDFR SCKHYGMEND 350
    DILGPVQSNG VYVSDLPFFG GQFIWKANPA IVEKLAEVGA LLKHESIQHS 400
    YMHCWRHKTP LIYRATAQWF VGMDKVAHDG SSLRRRALDA IEQTEFVPAW 450
    GQARLHGMIA GRPDWCISRQ RTWGVPIPFF LHKESGELHP RTVELMEQVA 500
    QRVEQGGIEA WSKLDAAELL GDEAAQYEKI TDTLDVWFDS GTTHWHVMRG 550
    SHPMGHEQGP RADLYLEGSD QHRGWFHSSL LTGAAIDGHA PYKGLLTHGF 600
    TVDENGRKMS KSLGNVIAPQ EITDSMGADI LRLWVSATDY SGEMAVSKQI 650
    LQRSADAYRR IRNTARFLLS NLDGFDPAQH MAPNDQLIAL DRWAIDRALL 700
    LQREIEEAYT TYKFWNVYQK VHNFCVQELG GFYLDIIKDR QYTTGADSLP 750
    RRSCQTALYH IAEALVRWIA PILAFTAEEI WQYLPGERNE SVMLNTWYEG 800
    LAELPEGFEL GRPFWDKVME VKAAVNKELE NQRNAKAIGG NLQAEVTLYA 850
    EESLAGELAR LGSELRFVLI TSAVDIAPLA QAPAEAVDSE LAGLKLLVRK 900
    TEHSKCGRCW HHLPDVGSHA EHPEICGRCV ENIEGAGEVR HYA 943
    Length:943
    Mass (Da):105,725
    Last modified:May 29, 2007 - v1
    Checksum:i8BFA739B16BB7195
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000304 Genomic DNA. Translation: ABP78661.1.
    RefSeqiYP_001171503.1. NC_009434.1.

    Genome annotation databases

    EnsemblBacteriaiABP78661; ABP78661; PST_0964.
    GeneIDi5098634.
    KEGGipsa:PST_0964.
    PATRICi19962355. VBIPseStu31643_0964.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000304 Genomic DNA. Translation: ABP78661.1 .
    RefSeqi YP_001171503.1. NC_009434.1.

    3D structure databases

    ProteinModelPortali A4VI60.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 379731.PST_0964.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABP78661 ; ABP78661 ; PST_0964 .
    GeneIDi 5098634.
    KEGGi psa:PST_0964.
    PATRICi 19962355. VBIPseStu31643_0964.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci PSTU379731:GJER-965-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of the metabolically versatile and root-associated nitrogen-fixing bacterium Pseudomonas stutzeri A1501."
      Yan Y., Yang J., Dou Y., Ping S., Chen M., Yao Z., Li H., Lu W., Zhang W., Peng J., Liu W., He S., Geng L., Zhang X., Yang F., Li D., Lin Z., Wang Y.
      , Elmerich C., Lin M., Jin Q.
      Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: A1501.

    Entry informationi

    Entry nameiSYI_PSEU5
    AccessioniPrimary (citable) accession number: A4VI60
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: May 29, 2007
    Last modified: October 1, 2014
    This is version 55 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3