Reviewed, UniProtKB/Swiss-Prot A4VI28 (RLMG_PSEU5)

Names and origin

Protein names

Recommended name:
    Ribosomal RNA large subunit methyltransferase G
    EC=2.1.1.52
Alternative name(s):
    rRNA (guanine-N(2)-)-methyltransferase rlmG
    23S rRNA m2G1835 methyltransferase

Gene names

Name:	rlmG
Ordered Locus Names:	PST_0932

Organism

Pseudomonas stutzeri (strain A1501) [Complete proteome] [HAMAP]

Taxonomic identifier

379731 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA By similarity. HAMAP MF_01859
Catalytic activity	S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing N(2)-methylguanine. HAMAP MF_01859
Subcellular location	Cytoplasm Potential HAMAP MF_01859.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmG family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA methylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro rRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 375	375	Ribosomal RNA large subunit methyltransferase G HAMAP MF_01859		PRO_0000366483

Sequences

	Sequence		Length	Mass (Da)
	A4VI28-1 [UniParc]. Last modified May 29, 2007. Version 1. Checksum: 8E6DC4850AB66640	FASTA	375	40,621
	10 20 30 40 50 60 MPILDTPYAS LDLIRQPEQP NEPLQAFDAA DEYLLATLHG QGLPAATRVL ILNDSFGALA 70 80 90 100 110 120 CALAAHVEVT SSGDSHLAHL ALQKNLARND LPADRVRFVP ASEAAQGPFD RVLIRVPKTL 130 140 150 160 170 180 SLLEEQLIRL HDQLAPGAQV IAAAMIKHLP RAAGDLLEQY IGPVQASLAV KKARLLSATP 190 200 210 220 230 240 VDKPAPQSPY PTRYQLDQPK LELLNHANLF CREGLDIGTR AFLPHLPKAL GAIRVADLGC 250 260 270 280 290 300 GNGVLGIVYA LGNPQAELTL VDESYMAVQS ARENWQVILG ERPADIRAGD GLAEQPPGSL 310 320 330 340 350 360 DLVLCNPPFH QQQVVGDFLA WRMFTQAKAA LTKGGELWIV GNRHLGYHLK LKRLFGNAEQ 370 VAATPKFVVL RSIKA

References

[1]

"Complete genome sequence of the metabolically versatile and root-associated nitrogen-fixing bacterium Pseudomonas stutzeri A1501." Yan Y., Yang J., Dou Y., Ping S., Chen M., Yao Z., Li H., Lu W., Zhang W., Peng J., Liu W., He S., Geng L., Zhang X., Yang F., Li D., Lin Z., Wang Y. , Elmerich C., Lin M., Jin Q. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000304 Genomic DNA. Translation: ABP78629.1.
RefSeq	YP_001171471.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A4VI28.
Genome annotation databases
GeneID	5098602.
GenomeReviews	Gene locus PST_0932 in contig CP000304_GR.
KEGG	psa:PST_0932.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2813.
HOGENOM	HBG321931.
OMA	CGNGVLG.
PhylomeDB	A4VI28.
Family and domain databases
HAMAP	MF_01859. 23SrRNA_methyltr_G. [Tree]
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR017237. rRNA_m2G-MeTrfase_RsmD. IPR007848. Small_mtfrase. [Graphical view]
Pfam	PF05175. MTS. 1 hit. [Graphical view]
PIRSF	PIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMG_PSEU5

Accession

Primary (citable) accession number: A4VI28

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	May 29, 2007
Last modified:	January 19, 2010
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)