ID   L_EBLV2                 Reviewed;        2127 AA.
AC   A4UHQ7;
DT   21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT   15-MAY-2007, sequence version 1.
DT   16-JUN-2009, entry version 16.
DE   RecName: Full=Large structural protein;
DE            Short=Protein L;
DE   AltName: Full=Transcriptase;
DE   AltName: Full=Replicase;
DE   Includes:
DE     RecName: Full=RNA-directed RNA polymerase;
DE              EC=2.7.7.48;
DE   Includes:
DE     RecName: Full=mRNA (guanine-N(7)-)-methyltransferase;
DE              EC=2.1.1.56;
DE   Includes:
DE     RecName: Full=mRNA guanylyltransferase;
DE              EC=2.7.7.-;
GN   Name=L;
OS   European bat lyssavirus 2 (strain Human/Scotland/RV1333/2002) (EBLV2).
OC   Viruses; ssRNA negative-strand viruses; Mononegavirales;
OC   Rhabdoviridae; Lyssavirus.
OX   NCBI_TaxID=453116;
OH   NCBI_TaxID=40674; Mammalia.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=17374776; DOI=10.1099/vir.0.82692-0;
RA   Marston D.A., McElhinney L.M., Johnson N., Muller T., Conzelmann K.K.,
RA   Tordo N., Fooks A.R.;
RT   "Comparative analysis of the full genome sequence of European bat
RT   lyssavirus type 1 and type 2 with other lyssaviruses and evidence for
RT   a conserved transcription termination and polyadenylation motif in the
RT   G-L 3' non-translated region.";
RL   J. Gen. Virol. 88:1302-1314(2007).
CC   -!- FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl
CC       transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A)
CC       synthetase activities. The viral mRNA guanylyl transferase
CC       displays a different biochemical reaction than the cellular
CC       enzyme. The template is composed of the viral RNA tightly
CC       encapsidated by the nucleoprotein (N). Functions either as
CC       transcriptase or as replicase. The transcriptase synthesizes
CC       subsequently five subgenomic RNAs, assuring their capping and
CC       polyadenylation by a stuttering mechanism. The replicase mode is
CC       dependent on intracellular N protein concentration. In this mode,
CC       the polymerase replicates the whole viral genome without
CC       recognizing the transcriptional signals (By similarity).
CC   -!- CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate
CC       + RNA(n+1).
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-
CC       adenosyl-L-homocysteine + m(7)G(5')pppR-RNA.
CC   -!- SUBUNIT: Interacts with the P protein (By similarity).
CC   -!- SUBCELLULAR LOCATION: Virion (Potential). Host cytoplasm (By
CC       similarity).
CC   -!- SIMILARITY: Belongs to the rhabdoviridae protein L family.
CC   -!- SIMILARITY: Contains 1 RdRp catalytic domain.
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DR   EMBL; EF157977; ABO65252.1; -; Genomic_RNA.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0019012; C:virion; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004482; F:mRNA (guanine-N7-)-methyltransferase activity; IEA:EC.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006370; P:mRNA capping; IEA:UniProtKB-KW.
DR   GO; GO:0006410; P:transcription, RNA-dependent; IEA:UniProtKB-KW.
DR   InterPro; IPR017234; RNA-dir_RNA_pol_rhabdovir.
DR   InterPro; IPR014023; RNA_pol_cat.
DR   InterPro; IPR001016; RNA_pol_L_viral.
DR   Pfam; PF00946; Paramyx_RNA_pol; 1.
DR   PIRSF; PIRSF037546; RNA_pol_RhabdoV_sub; 1.
DR   PROSITE; PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Cytoplasm; Methyltransferase;
KW   mRNA capping; mRNA processing; Multifunctional enzyme;
KW   Nucleotide-binding; Nucleotidyltransferase; RNA replication;
KW   RNA-directed RNA polymerase; S-adenosyl-L-methionine; Transferase;
KW   Virion.
FT   CHAIN         1   2127       Large structural protein.
FT                                /FTId=PRO_0000297835.
FT   DOMAIN      611    799       RdRp catalytic.
SQ   SEQUENCE   2127 AA;  242663 MW;  EC05E7C49284B0DD CRC64;
     MIDPLEVYDD PVDPVEPEID ARSNSVVPNI LRNSDYNLNS PLIEDPSKLM LDWLITGNKP
     ARLNLTDNSL RSYKVLKSYF KKLDVGSLRV GGLGAQSMMS LWLHGAHSES TRSRKCLSDL
     ALFYQRSAPI EKLLNYTLEN RGLAIPTDGV LSSLKKVNYD RAFGRYLGNL YSSYLFFHVI
     ILYMNALDWE EEKTILALWK DLNSVDIKKD QVKFRDQIWG SLLVTKDFVY SQSANCLFDR
     NYTLMLKDLF LSRFNSLLIL LSPPEPRYSE DLISQLCQLY IAGDNVLSTC GNSGYDVIKM
     LEPYVVNSLV QRAEGFRPMI HSLGDFPTFI KDKVSQLEGT FGPSARNFFF VLDQLDNIHD
     LVFVYGCYRH WGHPYIDYRK GLSKLYDQVH VKKVIDGDYQ ECLASDLAKR ILRWGFDKYS
     KWYLDPKLLE KDHPLIPYIQ TQTWPPKHIV DIVGNTWHKL PITQIFEIPE SMDPSEILDD
     KSHSFTRTKL ASWLSDHRGG PVPSEKVIIT ALSRPPVNPR EFLKSIDLGG LPDDDLIIGL
     KPKERELKIE GRFFALMSWN LRLYFVITEK LLANYILPLF DALTMTDNLN KVFKKLIDRV
     TGQGLQDYSR VTYAFHLDYE KWNNHQRLES TKDVFSVLDY VFGLKKVFSR THEFFQKSWV
     YYSDRSDLIG LWEDQIYCLD MSDGPTCWNG QDGGLEGLRQ KGWSLVSLLM IDRESQTRNT
     RTKILAQGDN QVLCPTYMLS SGLTQEGLIY ELDSISRNAL SIYRAIEEGA SKLGLIIKKE
     ETMCSYDFLI YGKTPLFRGN ILVPESKRWA RVSCISNDQI VNLANIMSTV STNALTVAQH
     SQSLIKPMRD FLLMAVQAVF HYLLFSPILK DRVYKILSAE GDNFLLAMSR IIYLDPSLGG
     VSGMSLGRFH IRQFSDPVSE GLAFWKEIWS SSSESWIHSL CQEAGNPDLG DRSLESFTRL
     LEDPTTLNIR GGASPTILLK EAIRKALYDE VDKVENSEFR EAILLSKTHR DNFILFLKSI
     EPLFPRFLSE LFSSSFLGIP ESIIGLIQNS RTIRRQFRRS LSRTLEESFF NSEIHGINRM
     TQVPQRVGRV WNCSAERADL LREISWGRKV VGTTVPHPGE MLVLLPKSSV SCVCKQTGED
     SPRISVSVLP SFDQSFFSRG PLKGYLGSST SMSTQLFHAW EKVTNVHVVK RALSLKESIN
     WFIARDSNLA QTLIRNITSL TGPQFPLEET PVFKRTGSAL HRFKSARYSE GGYSSICPNL
     LSHISVSTDT MSDLTQDGKN FDFMFQPLML YAQTWTSELV QKDIRLRDST FHWHLRCLKC
     IRPIDDIILD APQVFMFPDV SKRISRMVSG AVPQFQRLPE INLKPGKFEA LDSKDKSRHI
     GTAQGLLYSI LVAIHDSGYN DATIFPMNIY SKISPRDYLR GLSRGILIGS SICFLTRMTN
     ININRPLELI SGVISYILLR LDNHPSLYVM LREPSLRSEI FSIPQKIPAA YPTTMKEGNR
     SVLCYLQHVL RYERDVITAS PENDWLWIFS DFRSAKMTYL TLVTYQSHIL LQKIEKNLPK
     QMRIRLRQLS SLMRQILGGH GEDTLDSDED IQGLLRDALQ RTRWVDQEVR HAAKTMTGDH
     SPSKKVSRKA GCSEWICSAQ QVAISTSSNP APTSEMDVRA LSRRFQNPLI SGLRVVQWAT
     GAHYKLKPIL DNLEAYPSLC LVVGDGSGGI SRTVLSMFPD AKLVFNSLLE VNDFMASGTH
     PLPPSAIVSG GDDIVSRVID FGSIWEKPSD LRNLSTWRYF QSIQTVNNMS YDLIVCDAEV
     TDIPSVNKIT LLMSDFSLSI NGPLNLIFKT YGTMLVNPDY KAIQHLSRAF PSVTGYITQM
     TSSFSSELYL KFSKRGKFFR DAEYLTSSTL REMSLVLFNC SSPKSEMQRA RSLNYQDLVR
     GFPEEIISNP YNEMIITLID SEVESFLVHK MVDDLELQRG TLAKMSIIIA IVMVFSNRVF
     NVSKPLTDPM FYPPSDPKIL RHFNICCSTL MYLSTALGDV LNFARLHELY NNPITYYFKK
     QVIRGSIYLS WSWCDTTSVF KKVACNSNLS LSSHWIRLIY KIVRTTRLAG SSNDLSKEVE
     KHLKGYNRWI SFDDIRSRSS LLDYSCL
//