Reviewed, UniProtKB/Swiss-Prot A4UHQ7 (L_EBLV2)

Names and origin

Protein names

Recommended name:
    Large structural protein
      Short name=Protein L
Alternative name(s):
    Transcriptase
    Replicase
Including the following 3 domains:
    1- Recommended name:
            RNA-directed RNA polymerase
              EC=2.7.7.48
    2- Recommended name:
            mRNA (guanine-N(7)-)-methyltransferase
              EC=2.1.1.56
    3- Recommended name:
            mRNA guanylyltransferase
              EC=2.7.7.-

Gene names

Name:

L

Organism

European bat lyssavirus 2 (strain Human/Scotland/RV1333/2002) (EBLV2) [Complete proteome]

Taxonomic identifier

453116 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Rhabdoviridae › Lyssavirus

Virus host

Mammalia [TaxID: 40674]

Protein attributes

Sequence length	2127 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently five subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subunit structure	Interacts with the P protein By similarity.
Subcellular location	Virion Potential. Host cytoplasm By similarity.
Sequence similarities	Belongs to the rhabdoviridae protein L family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication mRNA capping mRNA processing
Cellular component	Cytoplasm Virion
Ligand	ATP-binding Nucleotide-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2127	2127	Large structural protein		PRO_0000297835
Regions
	Domain	611 – 799	189	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	A4UHQ7-1 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: EC05E7C49284B0DD	FASTA	2,127	242,663
	10 20 30 40 50 60 MIDPLEVYDD PVDPVEPEID ARSNSVVPNI LRNSDYNLNS PLIEDPSKLM LDWLITGNKP 70 80 90 100 110 120 ARLNLTDNSL RSYKVLKSYF KKLDVGSLRV GGLGAQSMMS LWLHGAHSES TRSRKCLSDL 130 140 150 160 170 180 ALFYQRSAPI EKLLNYTLEN RGLAIPTDGV LSSLKKVNYD RAFGRYLGNL YSSYLFFHVI 190 200 210 220 230 240 ILYMNALDWE EEKTILALWK DLNSVDIKKD QVKFRDQIWG SLLVTKDFVY SQSANCLFDR 250 260 270 280 290 300 NYTLMLKDLF LSRFNSLLIL LSPPEPRYSE DLISQLCQLY IAGDNVLSTC GNSGYDVIKM 310 320 330 340 350 360 LEPYVVNSLV QRAEGFRPMI HSLGDFPTFI KDKVSQLEGT FGPSARNFFF VLDQLDNIHD 370 380 390 400 410 420 LVFVYGCYRH WGHPYIDYRK GLSKLYDQVH VKKVIDGDYQ ECLASDLAKR ILRWGFDKYS 430 440 450 460 470 480 KWYLDPKLLE KDHPLIPYIQ TQTWPPKHIV DIVGNTWHKL PITQIFEIPE SMDPSEILDD 490 500 510 520 530 540 KSHSFTRTKL ASWLSDHRGG PVPSEKVIIT ALSRPPVNPR EFLKSIDLGG LPDDDLIIGL 550 560 570 580 590 600 KPKERELKIE GRFFALMSWN LRLYFVITEK LLANYILPLF DALTMTDNLN KVFKKLIDRV 610 620 630 640 650 660 TGQGLQDYSR VTYAFHLDYE KWNNHQRLES TKDVFSVLDY VFGLKKVFSR THEFFQKSWV 670 680 690 700 710 720 YYSDRSDLIG LWEDQIYCLD MSDGPTCWNG QDGGLEGLRQ KGWSLVSLLM IDRESQTRNT 730 740 750 760 770 780 RTKILAQGDN QVLCPTYMLS SGLTQEGLIY ELDSISRNAL SIYRAIEEGA SKLGLIIKKE 790 800 810 820 830 840 ETMCSYDFLI YGKTPLFRGN ILVPESKRWA RVSCISNDQI VNLANIMSTV STNALTVAQH 850 860 870 880 890 900 SQSLIKPMRD FLLMAVQAVF HYLLFSPILK DRVYKILSAE GDNFLLAMSR IIYLDPSLGG 910 920 930 940 950 960 VSGMSLGRFH IRQFSDPVSE GLAFWKEIWS SSSESWIHSL CQEAGNPDLG DRSLESFTRL 970 980 990 1000 1010 1020 LEDPTTLNIR GGASPTILLK EAIRKALYDE VDKVENSEFR EAILLSKTHR DNFILFLKSI 1030 1040 1050 1060 1070 1080 EPLFPRFLSE LFSSSFLGIP ESIIGLIQNS RTIRRQFRRS LSRTLEESFF NSEIHGINRM 1090 1100 1110 1120 1130 1140 TQVPQRVGRV WNCSAERADL LREISWGRKV VGTTVPHPGE MLVLLPKSSV SCVCKQTGED 1150 1160 1170 1180 1190 1200 SPRISVSVLP SFDQSFFSRG PLKGYLGSST SMSTQLFHAW EKVTNVHVVK RALSLKESIN 1210 1220 1230 1240 1250 1260 WFIARDSNLA QTLIRNITSL TGPQFPLEET PVFKRTGSAL HRFKSARYSE GGYSSICPNL 1270 1280 1290 1300 1310 1320 LSHISVSTDT MSDLTQDGKN FDFMFQPLML YAQTWTSELV QKDIRLRDST FHWHLRCLKC 1330 1340 1350 1360 1370 1380 IRPIDDIILD APQVFMFPDV SKRISRMVSG AVPQFQRLPE INLKPGKFEA LDSKDKSRHI 1390 1400 1410 1420 1430 1440 GTAQGLLYSI LVAIHDSGYN DATIFPMNIY SKISPRDYLR GLSRGILIGS SICFLTRMTN 1450 1460 1470 1480 1490 1500 ININRPLELI SGVISYILLR LDNHPSLYVM LREPSLRSEI FSIPQKIPAA YPTTMKEGNR 1510 1520 1530 1540 1550 1560 SVLCYLQHVL RYERDVITAS PENDWLWIFS DFRSAKMTYL TLVTYQSHIL LQKIEKNLPK 1570 1580 1590 1600 1610 1620 QMRIRLRQLS SLMRQILGGH GEDTLDSDED IQGLLRDALQ RTRWVDQEVR HAAKTMTGDH 1630 1640 1650 1660 1670 1680 SPSKKVSRKA GCSEWICSAQ QVAISTSSNP APTSEMDVRA LSRRFQNPLI SGLRVVQWAT 1690 1700 1710 1720 1730 1740 GAHYKLKPIL DNLEAYPSLC LVVGDGSGGI SRTVLSMFPD AKLVFNSLLE VNDFMASGTH 1750 1760 1770 1780 1790 1800 PLPPSAIVSG GDDIVSRVID FGSIWEKPSD LRNLSTWRYF QSIQTVNNMS YDLIVCDAEV 1810 1820 1830 1840 1850 1860 TDIPSVNKIT LLMSDFSLSI NGPLNLIFKT YGTMLVNPDY KAIQHLSRAF PSVTGYITQM 1870 1880 1890 1900 1910 1920 TSSFSSELYL KFSKRGKFFR DAEYLTSSTL REMSLVLFNC SSPKSEMQRA RSLNYQDLVR 1930 1940 1950 1960 1970 1980 GFPEEIISNP YNEMIITLID SEVESFLVHK MVDDLELQRG TLAKMSIIIA IVMVFSNRVF 1990 2000 2010 2020 2030 2040 NVSKPLTDPM FYPPSDPKIL RHFNICCSTL MYLSTALGDV LNFARLHELY NNPITYYFKK 2050 2060 2070 2080 2090 2100 QVIRGSIYLS WSWCDTTSVF KKVACNSNLS LSSHWIRLIY KIVRTTRLAG SSNDLSKEVE 2110 2120 KHLKGYNRWI SFDDIRSRSS LLDYSCL

References

[1]

"Comparative analysis of the full genome sequence of European bat lyssavirus type 1 and type 2 with other lyssaviruses and evidence for a conserved transcription termination and polyadenylation motif in the G-L 3' non-translated region." Marston D.A., McElhinney L.M., Johnson N., Muller T., Conzelmann K.K., Tordo N., Fooks A.R. J. Gen. Virol. 88:1302-1314(2007) [PubMed: 17374776] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	EF157977 Genomic RNA. Translation: ABO65252.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR017234. RNA-dir_RNA_pol_rhabdovir. IPR014023. RNA_pol_cat. IPR001016. RNA_pol_L_viral. [Graphical view]
Pfam	PF00946. Paramyx_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF037546. RNA_pol_RhabdoV_sub. 1 hit.
PROSITE	PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_EBLV2

Accession

Primary (citable) accession number: A4UHQ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 21, 2007
Last sequence update:	May 15, 2007
Last modified:	June 16, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)