ID   L_EBLV1                 Reviewed;        2127 AA.
AC   A4UHQ2;
DT   21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT   15-MAY-2007, sequence version 1.
DT   16-JUN-2009, entry version 17.
DE   RecName: Full=Large structural protein;
DE            Short=Protein L;
DE   AltName: Full=Transcriptase;
DE   AltName: Full=Replicase;
DE   Includes:
DE     RecName: Full=RNA-directed RNA polymerase;
DE              EC=2.7.7.48;
DE   Includes:
DE     RecName: Full=mRNA (guanine-N(7)-)-methyltransferase;
DE              EC=2.1.1.56;
DE   Includes:
DE     RecName: Full=mRNA guanylyltransferase;
DE              EC=2.7.7.-;
GN   Name=L;
OS   European bat lyssavirus 1 (strain Bat/Germany/RV9/1968) (EBLV1).
OC   Viruses; ssRNA negative-strand viruses; Mononegavirales;
OC   Rhabdoviridae; Lyssavirus.
OX   NCBI_TaxID=453115;
OH   NCBI_TaxID=40674; Mammalia.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=17374776; DOI=10.1099/vir.0.82692-0;
RA   Marston D.A., McElhinney L.M., Johnson N., Muller T., Conzelmann K.K.,
RA   Tordo N., Fooks A.R.;
RT   "Comparative analysis of the full genome sequence of European bat
RT   lyssavirus type 1 and type 2 with other lyssaviruses and evidence for
RT   a conserved transcription termination and polyadenylation motif in the
RT   G-L 3' non-translated region.";
RL   J. Gen. Virol. 88:1302-1314(2007).
CC   -!- FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl
CC       transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A)
CC       synthetase activities. The viral mRNA guanylyl transferase
CC       displays a different biochemical reaction than the cellular
CC       enzyme. The template is composed of the viral RNA tightly
CC       encapsidated by the nucleoprotein (N). Functions either as
CC       transcriptase or as replicase. The transcriptase synthesizes
CC       subsequently five subgenomic RNAs, assuring their capping and
CC       polyadenylation by a stuttering mechanism. The replicase mode is
CC       dependent on intracellular N protein concentration. In this mode,
CC       the polymerase replicates the whole viral genome without
CC       recognizing the transcriptional signals (By similarity).
CC   -!- CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate
CC       + RNA(n+1).
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-
CC       adenosyl-L-homocysteine + m(7)G(5')pppR-RNA.
CC   -!- SUBUNIT: Interacts with the P protein (By similarity).
CC   -!- SUBCELLULAR LOCATION: Virion (Potential). Host cytoplasm (By
CC       similarity).
CC   -!- SIMILARITY: Belongs to the rhabdoviridae protein L family.
CC   -!- SIMILARITY: Contains 1 RdRp catalytic domain.
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DR   EMBL; EF157976; ABO65247.1; -; Genomic_RNA.
DR   RefSeq; YP_001285392.1; -.
DR   GeneID; 5219906; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0019012; C:virion; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004482; F:mRNA (guanine-N7-)-methyltransferase activity; IEA:EC.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006370; P:mRNA capping; IEA:UniProtKB-KW.
DR   GO; GO:0006410; P:transcription, RNA-dependent; IEA:UniProtKB-KW.
DR   InterPro; IPR017234; RNA-dir_RNA_pol_rhabdovir.
DR   InterPro; IPR014023; RNA_pol_cat.
DR   InterPro; IPR001016; RNA_pol_L_viral.
DR   Pfam; PF00946; Paramyx_RNA_pol; 1.
DR   PIRSF; PIRSF037546; RNA_pol_RhabdoV_sub; 1.
DR   PROSITE; PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Cytoplasm; Methyltransferase;
KW   mRNA capping; mRNA processing; Multifunctional enzyme;
KW   Nucleotide-binding; Nucleotidyltransferase; RNA replication;
KW   RNA-directed RNA polymerase; S-adenosyl-L-methionine; Transferase;
KW   Virion.
FT   CHAIN         1   2127       Large structural protein.
FT                                /FTId=PRO_0000297834.
FT   DOMAIN      611    799       RdRp catalytic.
SQ   SEQUENCE   2127 AA;  242548 MW;  D26808658BFD089E CRC64;
     MIDSTEVYDD PVDPVEPEAD LRSNSVVPNI LRNSDYNLNS PLLEDPSRLM IEWLTTGNRP
     SRLNLTDNLL RSYKVLRGYF KKLDVGSMRA GGLGAQAMIT LWLHGSHSES TRSRKCLTEL
     SQFYKKSSPI EKLLNYTLEN RGLKTPTEGV LSSINKVQYD QAFGRYLGNT YSSYLFFHVI
     ILYMNALDWD EEKTILALWK EIASIDVKSD LVKFRDQIWG SLVITKDFVY SQSANCLFDR
     NYTLMLKDLF LSRFNSLLIL ISPPESRYSD DLVSNLCQLY IAGDKVLSAC GNSGYDVIKL
     LEPYIVNKLV QKAEKFRPLI HSLGDFPQFI RDKTNQLEGT FGPSAREFFQ TMDLLDNIHD
     LVFVYGCYRH WGHPYIDYRK GLSKLYDQVH VKKIIDRNYQ ECLASDLAKR ILRWGFDKYS
     RWYLDSNLLP GDHPLSPYVK TQTWPPKHVV DMVGDTWHSL PITQIFEIPE SMDPSEILDD
     KSHSFTRTRL ASWLSENRGG PVPSEKVIIT ALSRPPVNPR EFLRSIDVGG LPDDDLIIGL
     KPKERELKIE GRFFALMSWN LRLYFVITEK LLATYILPLF DALTMTDNLN KVFKKLIDRV
     TGQGLLDYSR VTYAFHLDYE KWNNHQRLES TKDVFSVLDK VFGLKKVFSR THEFFQKSWV
     YYSDRSDLIG LWRDQIYCLD MTNGPTCWNG QDGGLEGLRQ KGWSLVSLLM IDRESQTRNT
     RTKILAQGDN QVLCPTYMLS SGLNKEGLLY ELDSISRNAI SIYRAIEDGA SKLGLIIKKE
     ETMCSYDFLI YGKTPLFRGN ILVPESKRWA RVSCISNDQI VNLANIMSTV STNALTVAQH
     SQSLIKPMRD FLLMSVQAVY HYLLFSPILK DRVYKILVAD GTEFLLAMSR IIYLDPSLGG
     VSGMSLGRFH IRQFSDPVSE GLSFWREIWL SSSESWIHAL CQEAGNPDLG DRSLESFTRL
     LEDPTTLNIR GGASPTILLR EAIRKALYDE VDKVENSEFR EAILLSKTHR DNFILFLRSV
     EPLFPRFLSE LFSSSFLGIP ESIIGLIQNS RTIRRQFRRS LSRTLEESFF NSEIHGISRM
     TQVPQRIGRV WSCSAERADQ LREISWGRKV VGTTVPHPSE MLALIPKSSI SCTCGQSGDD
     SPRISVSVLP SLDQSFFSRG PLKGYLGSST SMSTQLFHAW EKVTNVHVVK RALSLKESIN
     WFITRDSNLA QTLIRNIVSL TGPFFPLEEA PVFKRTGSAL HRFKSARYSE GGYSSICPNL
     LSHISVSTDT MSDLTQDGTN FDFMFQPLML YAQTWTSELV QKDLRLRDST FHWHLRCLKC
     IRPIDDITLE APKIFSFPDV SKRISRMVSG AVPQFRKLPE IGLKPGKFDS LKEKDKSRHI
     GTAQGLLYSI LVATHDSGYN DGTIFPVNIY GKVSPRDYLR GLARGVLIGS SICFLTRMTN
     ININRPLELI SGVISYILLR LDNHPSLYVM LKEPSLRSEI FSIPQKIPAA YPTTMKEGNR
     SVLCYLQHVL RYEREAITTS PENDWLWIFS DFRSIKMTYL TLITYQSHLL LQRIDRNLSK
     QMRVRLRQLN SLMRQVLGGH GEGTLDSDED IQGLLRDALQ RTRWVDQEVR HAAKTMKCDY
     SPSKRVSRKA GCSEWICSAQ QVAVSTSSNP APISEIDIRA LSKKLQNPLI SGLRVVQWAT
     GAHYKLKPIL NDLEAYPTLC LVVGDGSGGI SRAVLSMFPD AKLVFNSLLE VNDLMASGTH
     PLPPSALMSG GEDIVSRVID FNSIWEKPSD LRNPSTWRYF QTVQSAANMS YDLIICDAEV
     TDIPSINKIT LLMSDFSLSI NGPLSLIFKT YGTMLVNPDY KAVQHLSRAF PSVTGYITQM
     TSSFSSELYL KFSKRGKFFR DSEYLTSSTL REMSLVLFNC SSSKSEMLRA RSLNYQDLVR
     GFPEEIISNP YNEMIITLID NDVESFLVHK MVDDLELRQG AFSKMSIILT IMMVFSNRVF
     NVSKPLNDPK FYPPSDPKIL RHFNICCGTL IYLSAALGDV LNFARLHELY NNPVTYYFKK
     QTLGGRMYLA WSWTDNTPVF KRVACNSSLS LSSHWIRLIY KIVKTTRLVG SAKDLSQEVE
     KHLKSYNRWI NFSDLRSRSS LLDYSCL
//