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A4U7B4 (RDRP_I51A0) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 31. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RNA-directed RNA polymerase catalytic subunit

EC=2.7.7.48
Alternative name(s):
Polymerase basic protein 1
Short name=PB1
RNA-directed RNA polymerase subunit P1
Gene names
Name:PB1
OrganismInfluenza A virus (strain A/USA:Albany/12/1951 H1N1) [Complete proteome]
Taxonomic identifier425580 [NCBI]
Taxonomic lineageVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length757 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

RNA-dependent RNA polymerase which is responsible for replication and transcription of virus segments. Binds the promoter sequence of the encapsidated viral RNA. Displays an endonuclease activity involved in cap-stealing. Cleaves cellular pre-mRNA to generate primers for viral transcription By similarity.

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Subunit structure

Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA By similarity.

Subcellular location

Host nucleus Potential.

Sequence similarities

Belongs to the influenza viruses polymerase PB1 family.

Contains 1 RdRp catalytic domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 757757RNA-directed RNA polymerase catalytic subunit
PRO_0000373051

Regions

Domain286 – 483198RdRp catalytic
Region249 – 2568Promoter-binding site By similarity
Motif187 – 1959Nuclear localization signal By similarity
Motif203 – 21614Nuclear localization signal By similarity

Sequences

Sequence LengthMass (Da)Tools
A4U7B4 [UniParc].

Last modified May 15, 2007. Version 1.
Checksum: 6D738174E4936E91

FASTA75786,565
        10         20         30         40         50         60 
MDVNPTLLFL KVPAQNAIST TFPYTGDPPY SHGTGTGYTM DTVNRTHQYS ERGRWTKNTE 

        70         80         90        100        110        120 
TGAPQLNPID GPLPEDNEPS GYAQTDCVLE AMAFLEESHP GIFENSCIET MEVVQQTRVD 

       130        140        150        160        170        180 
KLTQGRQTYD WTLNRNQPAA TALANTIEVF RSNGLMANES GRLIDFLKDV MESMDKEEME 

       190        200        210        220        230        240 
VTTHFQRKRR VRDNVTKKMV TQRTIGKKKQ RLNKRSYLIR ALTLNTMTKD AERGKLKRRA 

       250        260        270        280        290        300 
IATPGMQIRG FVYFVETLAR SICEKLEQSG LPVGGNEKKA KLANVVRKMM TNSQDTEISF 

       310        320        330        340        350        360 
TITGDNTKWN ENQNPRMFLA MITYITRNQP EWFRNILSIA PIMFSNKMAR LGKGYMFESK 

       370        380        390        400        410        420 
SMKLRTQIPA EMIANIDLKY FNDSTRKKIE KIRPLLIDGT ASLSPGMMMG MFNMLSTVLG 

       430        440        450        460        470        480 
VSILNLGQKR YTKTTYWWDG LQSSDDFALI VNAPNHAGIQ AGVDRFYRTC KLLGINMSKK 

       490        500        510        520        530        540 
KSYINRTGTF EFTSFFYRYG FVANFSMELP SFGVSGINES ADMSIGVTVI KNNMINNDLG 

       550        560        570        580        590        600 
PATAQMALQL FIKDYRYTYR CHRGDTQIQT RRSFEIKKLW EQTRSKAGLL VSDGGPNLYN 

       610        620        630        640        650        660 
IRNLHIPEVC LKWELMDEDY QGRLCNPLNP FVSHKEIESV NNAVMMPAHG PAKNMEYDAV 

       670        680        690        700        710        720 
ATTHSWVPKR NRSILNTSQR GILEDEQMYQ RCCNLFEKFF PSSSYRRPVG ISSMVEAMVS 

       730        740        750 
RARIDARIDF ESGRIKKEDF TEIMKICSTI EELRRQK 

« Hide

References

[1]"The NIAID influenza genome sequencing project."
Spiro D., Sengamalay N., Boyne A., Bera J., Ghedin E., Zaborsky J., Subbu V., Sparenborg J., Gallagher T., Overton L., Althoff R., Liu X., Sitz J., Katzel D., Neupane R., Shumway M., Koo H., Griesemer S. expand/collapse author list , StGeorge K., Bennett R., Taylor J., Bao Y., Bolotov P., Dernovoy D., Kiryutin B., Lipman D.J., Tatusova T.
Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]The NIAID Influenza Genome Sequencing Consortium
Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CY021827 Viral cRNA. Translation: ABP49489.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR007099. RNA-dir_pol_NSvirus.
IPR001407. RNA_pol_PB1_influenza.
[Graphical view]
PfamPF00602. Flu_PB1. 1 hit.
[Graphical view]
PIRSFPIRSF000827. RdRPol_OMV. 1 hit.
PROSITEPS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRDRP_I51A0
AccessionPrimary (citable) accession number: A4U7B4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 15, 2007
Last modified: July 9, 2014
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families