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Protein

RNA-directed RNA polymerase catalytic subunit

Gene

PB1

Organism
Influenza A virus (strain A/USA:Huston/AA/1945 H1N1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

RNA-dependent RNA polymerase which is responsible for replication and transcription of virus RNA segments. The transcription of viral mRNAs occurs by a unique mechanism called cap-snatching. 5' methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides by PA. In turn, these short capped RNAs are used as primers by PB1 for transcription of viral mRNAs. During virus replication, PB1 initiates RNA synthesis and copy vRNA into complementary RNA (cRNA) which in turn serves as a template for the production of more vRNAs.UniRule annotation

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, RNA-directed RNA polymerase, Transferase
Biological processEukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host RNA polymerase II by virus, Viral RNA replication, Viral transcription
LigandNucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase catalytic subunitUniRule annotation (EC:2.7.7.48UniRule annotation)
Alternative name(s):
Polymerase basic protein 1UniRule annotation
Short name:
PB1UniRule annotation
RNA-directed RNA polymerase subunit P1UniRule annotation
Gene namesi
Name:PB1UniRule annotation
OrganismiInfluenza A virus (strain A/USA:Huston/AA/1945 H1N1)
Taxonomic identifieri425551 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesOrthomyxoviridaeAlphainfluenzavirus
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]
Proteomesi
  • UP000008433 Componenti: Genome

Subcellular locationi

  • Host nucleus UniRule annotation
  • Host cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm, Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003730501 – 757RNA-directed RNA polymerase catalytic subunitAdd BLAST757

Post-translational modificationi

Phosphorylated by host PRKCA.UniRule annotation

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiA4U6W0

Interactioni

Subunit structurei

Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA. Interacts (via N-terminus) with PA (via C-terminus). Interacts (via C-terminus) with PB2 (via N-terminus); this interaction is essential for transcription initiation.UniRule annotation

Structurei

3D structure databases

SMRiA4U6W0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini286 – 483RdRp catalyticUniRule annotationAdd BLAST198

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni249 – 256Promoter-binding siteUniRule annotation8

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi187 – 195Nuclear localization signalUniRule annotation9
Motifi203 – 216Nuclear localization signalUniRule annotationAdd BLAST14

Sequence similaritiesi

Belongs to the influenza viruses polymerase PB1 family.UniRule annotation

Phylogenomic databases

OrthoDBiVOG09000028

Family and domain databases

HAMAPiMF_04065 INFV_RDRP, 1 hit
InterProiView protein in InterPro
IPR007099 RNA-dir_pol_NSvirus
IPR001407 RNA_pol_PB1_influenza
PfamiView protein in Pfam
PF00602 Flu_PB1, 1 hit
PIRSFiPIRSF000827 RdRPol_OMV, 1 hit
PROSITEiView protein in PROSITE
PS50525 RDRP_SSRNA_NEG_SEG, 1 hit

Sequencei

Sequence statusi: Complete.

A4U6W0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVNPILLFL KVPAQNAIST TFPYTGDPPY SHGTGTGYTM DTVNRTHQYS
60 70 80 90 100
ERGKWTTNTE TGAPQLNPID GPLPEDNEPS GYAQTDCVLE AMAFLEESHP
110 120 130 140 150
GIFENSCIET MEVVQQTRVD KLTQGRQTYD WTLNRNQPAA TALANTIEVF
160 170 180 190 200
RSNGLTANES GRLIDFLKDV MESMDKEEIE ITTHFQRKRR VRDNVTKKMV
210 220 230 240 250
TQRTIGKRKQ RLNKRSYLIR ALTLNTMTKD AERGKLKRRA IATPGMQIRG
260 270 280 290 300
FVYFVETLAR SICEKLEQSG LPVGGNEKKA KLANVVRKMM TNSQDTEISF
310 320 330 340 350
TITGDNTKWN ENQNPRMFLA MITYITRTQP EWFRNVLSIA PIMFSNKMAR
360 370 380 390 400
LGKGYMFESK SMKLRTQIPA EMLANIDLKY FNDSTRKKIE KIRPLLIDGT
410 420 430 440 450
ASLSPGMMMG MFNMLSTVLG VSILNLGQKR YTKTTYWWDG LQSSDDFALI
460 470 480 490 500
VNAPNHEGIQ AGVDRFYRTC KLLGINMSKK KSYINRTGTF EFTSFFYRYG
510 520 530 540 550
FVANFSMELP SFGVSGINES ADMSIGVTVI KNNMINNDLG PATAQMALQL
560 570 580 590 600
FIKDYRYTYR CHRGDTQIQT RRSFEIKKLW EQTRSKAGLL VSDGGPNLYN
610 620 630 640 650
IRNLHIPEVC LKWELMDEDY QGRLCNPLNP FVSHKEIESV NNAVMMPAHG
660 670 680 690 700
PAKNMEYDAV ATTHSWIPKR NRSILNTSQR GILEDEQMYQ RCCNLFEKFF
710 720 730 740 750
PSSSYRRPVG ISSMVEAMVS RARIDARIDF ESGRIKKEEF TEIMKICSTI

EELRRQK
Length:757
Mass (Da):86,575
Last modified:May 15, 2007 - v1
Checksum:i77D0FA36B4BFD89F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CY021715 Viral cRNA Translation: ABP49335.1

Entry informationi

Entry nameiRDRP_I45A0
AccessioniPrimary (citable) accession number: A4U6W0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 15, 2007
Last modified: May 23, 2018
This is version 41 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health