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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Yersinia pestis (strain Pestoides F)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei561Aminoacyl-adenylateUniRule annotation1
Binding sitei605ATPUniRule annotation1
Metal bindingi901ZincUniRule annotation1
Metal bindingi904ZincUniRule annotation1
Metal bindingi921ZincUniRule annotation1
Metal bindingi924ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:YPDSF_3157
OrganismiYersinia pestis (strain Pestoides F)
Taxonomic identifieri386656 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeYersinia

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000221461 – 938Isoleucine--tRNA ligaseAdd BLAST938

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA4TQF3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi58 – 68"HIGH" regionAdd BLAST11
Motifi602 – 606"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000246402.
KOiK01870.
OMAiPIPFFLH.

Family and domain databases

CDDicd07960. Anticodon_Ia_Ile_BEm. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033708. Anticodon_Ile_BEm.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_FPG/IleRS.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4TQF3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDYKNTLNL PETGFPMRGD LAKREPDMLK RWYEQDLYGI IRAAKKGKKT
60 70 80 90 100
FILHDGPPYA NGNIHIGHSV NKILKDIIVK SKGMAGYDSP YIPGWDCHGL
110 120 130 140 150
PIELKVEQLI GKPGEKVSAA EFRTACRKYA AEQVEGQKKD FIRLGVLGDW
160 170 180 190 200
DHPYLTMDFK TEANIIRALS KIIDNGHLHK GAKPVHWCTD CGSSLAEAEV
210 220 230 240 250
EYYDKTSQSI DVRFNAVDTA TVAAKFGVSA VNGPISLVIW TTTPWTLPAN
260 270 280 290 300
RAISLNAEYL YQLVQVEGEC LILAADLVES VMKRAGITQW AVLGSCTGSD
310 320 330 340 350
LELLRFTHPF MGFDVPAILG DHVTLDAGTG AVHTAPGHGP DDFVIGQKYG
360 370 380 390 400
LEVANPVGPN GCYLAGTYPT LDGLFVFKAN DVVVELLREK GALLHVEKLL
410 420 430 440 450
HSYPCCWRHK TPIIFRATPQ WFISMDQKGL RKQSLQEIKG VQWIPDWGQA
460 470 480 490 500
RIETMVANRP DWCISRQRTW GVPMSLFVHK ETEQLHPRSI ELMEEVAKRV
510 520 530 540 550
EQDGIQAWWD LDPAEILGAD AADYVKVPDT LDVWFDSGST HSSVVDVRPE
560 570 580 590 600
FGGHSPDMYL EGSDQHRGWF MSSLMIATAM KGKAPYRQVL THGFTVDGQG
610 620 630 640 650
RKMSKSIGNT ISPQDVMNKL GGDILRLWVA STDYTGEIAV SDEILKRSAD
660 670 680 690 700
SYRRIRNTAR FLLANLNGFD PAQHQVKPEE MVVVDRWAVG RAQAAQAEIM
710 720 730 740 750
EAYENYDFHL VVQRLMQFCS VEMGSFYLDI IKDRQYTAKG DGIARRSCQT
760 770 780 790 800
ALFHIAEALV RWMAPIMSFT ADEIWNHLPG ERQQYVFTEE WYDGLFGLAG
810 820 830 840 850
NESMNDTFWA ELLKVRGEVN KVLEQARSDK RIGGSLEAAV TLYAEPELAA
860 870 880 890 900
RLNSLQDELR FVLLTSAAKV AAYADAGNDA QQSELIAGLK ITFNKADGEK
910 920 930
CPRCWHYTQD VGLVAEHAEL CGRCVTNVAG DGEERKFA
Length:938
Mass (Da):104,690
Last modified:May 15, 2007 - v1
Checksum:i827A66208EE0B84A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000668 Genomic DNA. Translation: ABP41515.1.
RefSeqiWP_002210509.1. NZ_CP009715.1.

Genome annotation databases

EnsemblBacteriaiABP41515; ABP41515; YPDSF_3157.
KEGGiypp:YPDSF_3157.
PATRICi18616183. VBIYerPes122972_3968.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000668 Genomic DNA. Translation: ABP41515.1.
RefSeqiWP_002210509.1. NZ_CP009715.1.

3D structure databases

ProteinModelPortaliA4TQF3.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABP41515; ABP41515; YPDSF_3157.
KEGGiypp:YPDSF_3157.
PATRICi18616183. VBIYerPes122972_3968.

Phylogenomic databases

HOGENOMiHOG000246402.
KOiK01870.
OMAiPIPFFLH.

Family and domain databases

CDDicd07960. Anticodon_Ia_Ile_BEm. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033708. Anticodon_Ile_BEm.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_FPG/IleRS.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYI_YERPP
AccessioniPrimary (citable) accession number: A4TQF3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 15, 2007
Last modified: November 2, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.