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A4TPZ8

- ISPF_YERPP

UniProt

A4TPZ8 - ISPF_YERPP

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Protein
2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
Gene
ispF, YPDSF_3000
Organism
Yersinia pestis (strain Pestoides F)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in the biosynthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), two major building blocks of isoprenoid compounds. Catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP) By similarity.UniRule annotation

Catalytic activityi

2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP.UniRule annotation

Cofactori

Binds 1 divalent metal cation per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi8 – 81Divalent metal cation By similarity
Metal bindingi10 – 101Divalent metal cation By similarity
Sitei36 – 361Transition state stabilizer By similarity
Metal bindingi44 – 441Divalent metal cation By similarity
Binding sitei67 – 671Substrate; via carbonyl oxygen By similarity
Sitei135 – 1351Transition state stabilizer By similarity
Binding sitei141 – 1411Substrate; via carbonyl oxygen By similarity
Binding sitei144 – 1441Substrate By similarity

GO - Molecular functioni

  1. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway Source: UniProtKB-UniPathway
  2. terpenoid biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Isoprene biosynthesis

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

BioCyciYPES386656:GKD7-3063-MONOMER.
UniPathwayiUPA00056; UER00095.

Names & Taxonomyi

Protein namesi
Recommended name:
2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (EC:4.6.1.12)
Short name:
MECDP-synthase
Short name:
MECPP-synthase
Short name:
MECPS
Gene namesi
Name:ispF
Ordered Locus Names:YPDSF_3000
OrganismiYersinia pestis (strain Pestoides F)
Taxonomic identifieri386656 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
ProteomesiUP000000236: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 1621622-C-methyl-D-erythritol 2,4-cyclodiphosphate synthaseUniRule annotation
PRO_1000022895Add
BLAST

Interactioni

Subunit structurei

Homotrimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi386656.YPDSF_3000.

Structurei

3D structure databases

ProteinModelPortaliA4TPZ8.
SMRiA4TPZ8. Positions 1-157.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni8 – 103Substrate binding By similarity
Regioni36 – 372Substrate binding By similarity
Regioni40 – 489Substrate binding By similarity
Regioni58 – 603Substrate binding By similarity
Regioni63 – 675Substrate binding By similarity
Regioni102 – 1087Substrate binding By similarity
Regioni133 – 1375Substrate binding By similarity

Sequence similaritiesi

Belongs to the IspF family.

Phylogenomic databases

eggNOGiCOG0245.
HOGENOMiHOG000239175.
KOiK01770.
OMAiKGYQLGN.
OrthoDBiEOG6J48RZ.

Family and domain databases

Gene3Di3.30.1330.50. 1 hit.
HAMAPiMF_00107. IspF.
InterProiIPR003526. MECDP_synthase.
IPR020555. MECDP_synthase_CS.
[Graphical view]
PfamiPF02542. YgbB. 1 hit.
[Graphical view]
SUPFAMiSSF69765. SSF69765. 1 hit.
TIGRFAMsiTIGR00151. ispF. 1 hit.
PROSITEiPS01350. ISPF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4TPZ8-1 [UniParc]FASTAAdd to Basket

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MRIGHGFDVH KFGENGSGPL IIGGVRIPYE KGLLAHSDGD VALHAATDAL    50
LGAAALGDIG KLFPDTDPAF KGADSRGLLR EAYRRILAKG YKLGNLDITI 100
IAQAPKMAPH IPQMRVNLAE DLQCHMDDIN VKATTTEQLG FTGRGEGIAC 150
EAVVLLVNVE QG 162
Length:162
Mass (Da):17,182
Last modified:May 15, 2007 - v1
Checksum:i9A295411D3DFB1EB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000668 Genomic DNA. Translation: ABP41360.1.
RefSeqiYP_001164333.1. NC_009381.1.

Genome annotation databases

EnsemblBacteriaiABP41360; ABP41360; YPDSF_3000.
GeneIDi5062891.
KEGGiypp:YPDSF_3000.
PATRICi18615821. VBIYerPes122972_3789.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000668 Genomic DNA. Translation: ABP41360.1 .
RefSeqi YP_001164333.1. NC_009381.1.

3D structure databases

ProteinModelPortali A4TPZ8.
SMRi A4TPZ8. Positions 1-157.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 386656.YPDSF_3000.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABP41360 ; ABP41360 ; YPDSF_3000 .
GeneIDi 5062891.
KEGGi ypp:YPDSF_3000.
PATRICi 18615821. VBIYerPes122972_3789.

Phylogenomic databases

eggNOGi COG0245.
HOGENOMi HOG000239175.
KOi K01770.
OMAi KGYQLGN.
OrthoDBi EOG6J48RZ.

Enzyme and pathway databases

UniPathwayi UPA00056 ; UER00095 .
BioCyci YPES386656:GKD7-3063-MONOMER.

Family and domain databases

Gene3Di 3.30.1330.50. 1 hit.
HAMAPi MF_00107. IspF.
InterProi IPR003526. MECDP_synthase.
IPR020555. MECDP_synthase_CS.
[Graphical view ]
Pfami PF02542. YgbB. 1 hit.
[Graphical view ]
SUPFAMi SSF69765. SSF69765. 1 hit.
TIGRFAMsi TIGR00151. ispF. 1 hit.
PROSITEi PS01350. ISPF. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Pestoides F.

Entry informationi

Entry nameiISPF_YERPP
AccessioniPrimary (citable) accession number: A4TPZ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 15, 2007
Last modified: May 14, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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