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A4TPZ8

- ISPF_YERPP

UniProt

A4TPZ8 - ISPF_YERPP

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Protein

2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene

ispF

Organism
Yersinia pestis (strain Pestoides F)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in the biosynthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), two major building blocks of isoprenoid compounds. Catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP).UniRule annotation

Catalytic activityi

2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP.UniRule annotation

Cofactori

Binds 1 divalent metal cation per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi8 – 81Divalent metal cationUniRule annotation
Metal bindingi10 – 101Divalent metal cationUniRule annotation
Sitei36 – 361Transition state stabilizerUniRule annotation
Metal bindingi44 – 441Divalent metal cationUniRule annotation
Binding sitei67 – 671Substrate; via carbonyl oxygenUniRule annotation
Sitei135 – 1351Transition state stabilizerUniRule annotation
Binding sitei141 – 1411Substrate; via carbonyl oxygenUniRule annotation
Binding sitei144 – 1441SubstrateUniRule annotation

GO - Molecular functioni

  1. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway Source: UniProtKB-UniPathway
  2. terpenoid biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Isoprene biosynthesis

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

BioCyciYPES386656:GKD7-3063-MONOMER.
UniPathwayiUPA00056; UER00095.

Names & Taxonomyi

Protein namesi
Recommended name:
2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthaseUniRule annotation (EC:4.6.1.12UniRule annotation)
Short name:
MECDP-synthaseUniRule annotation
Short name:
MECPP-synthaseUniRule annotation
Short name:
MECPSUniRule annotation
Gene namesi
Name:ispFUniRule annotation
Ordered Locus Names:YPDSF_3000
OrganismiYersinia pestis (strain Pestoides F)
Taxonomic identifieri386656 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
ProteomesiUP000000236: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 1621622-C-methyl-D-erythritol 2,4-cyclodiphosphate synthasePRO_1000022895Add
BLAST

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi386656.YPDSF_3000.

Structurei

3D structure databases

ProteinModelPortaliA4TPZ8.
SMRiA4TPZ8. Positions 1-157.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni8 – 103Substrate bindingUniRule annotation
Regioni36 – 372Substrate bindingUniRule annotation
Regioni40 – 489Substrate bindingUniRule annotation
Regioni58 – 603Substrate bindingUniRule annotation
Regioni63 – 675Substrate bindingUniRule annotation
Regioni102 – 1087Substrate bindingUniRule annotation
Regioni133 – 1375Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the IspF family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0245.
HOGENOMiHOG000239175.
KOiK01770.
OMAiKGYQLGN.
OrthoDBiEOG6J48RZ.

Family and domain databases

Gene3Di3.30.1330.50. 1 hit.
HAMAPiMF_00107. IspF.
InterProiIPR003526. MECDP_synthase.
IPR020555. MECDP_synthase_CS.
[Graphical view]
PfamiPF02542. YgbB. 1 hit.
[Graphical view]
SUPFAMiSSF69765. SSF69765. 1 hit.
TIGRFAMsiTIGR00151. ispF. 1 hit.
PROSITEiPS01350. ISPF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4TPZ8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRIGHGFDVH KFGENGSGPL IIGGVRIPYE KGLLAHSDGD VALHAATDAL
60 70 80 90 100
LGAAALGDIG KLFPDTDPAF KGADSRGLLR EAYRRILAKG YKLGNLDITI
110 120 130 140 150
IAQAPKMAPH IPQMRVNLAE DLQCHMDDIN VKATTTEQLG FTGRGEGIAC
160
EAVVLLVNVE QG
Length:162
Mass (Da):17,182
Last modified:May 15, 2007 - v1
Checksum:i9A295411D3DFB1EB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000668 Genomic DNA. Translation: ABP41360.1.
RefSeqiYP_001164333.1. NC_009381.1.

Genome annotation databases

EnsemblBacteriaiABP41360; ABP41360; YPDSF_3000.
GeneIDi5062891.
KEGGiypp:YPDSF_3000.
PATRICi18615821. VBIYerPes122972_3789.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000668 Genomic DNA. Translation: ABP41360.1 .
RefSeqi YP_001164333.1. NC_009381.1.

3D structure databases

ProteinModelPortali A4TPZ8.
SMRi A4TPZ8. Positions 1-157.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 386656.YPDSF_3000.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABP41360 ; ABP41360 ; YPDSF_3000 .
GeneIDi 5062891.
KEGGi ypp:YPDSF_3000.
PATRICi 18615821. VBIYerPes122972_3789.

Phylogenomic databases

eggNOGi COG0245.
HOGENOMi HOG000239175.
KOi K01770.
OMAi KGYQLGN.
OrthoDBi EOG6J48RZ.

Enzyme and pathway databases

UniPathwayi UPA00056 ; UER00095 .
BioCyci YPES386656:GKD7-3063-MONOMER.

Family and domain databases

Gene3Di 3.30.1330.50. 1 hit.
HAMAPi MF_00107. IspF.
InterProi IPR003526. MECDP_synthase.
IPR020555. MECDP_synthase_CS.
[Graphical view ]
Pfami PF02542. YgbB. 1 hit.
[Graphical view ]
SUPFAMi SSF69765. SSF69765. 1 hit.
TIGRFAMsi TIGR00151. ispF. 1 hit.
PROSITEi PS01350. ISPF. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Pestoides F.

Entry informationi

Entry nameiISPF_YERPP
AccessioniPrimary (citable) accession number: A4TPZ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: May 15, 2007
Last modified: October 1, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3