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A4TPZ8

- ISPF_YERPP

UniProt

A4TPZ8 - ISPF_YERPP

Protein

2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene

ispF

Organism
Yersinia pestis (strain Pestoides F)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 55 (01 Oct 2014)
      Sequence version 1 (15 May 2007)
      Previous versions | rss
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    • Comment

    Functioni

    Involved in the biosynthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), two major building blocks of isoprenoid compounds. Catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP).UniRule annotation

    Catalytic activityi

    2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP.UniRule annotation

    Cofactori

    Binds 1 divalent metal cation per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi8 – 81Divalent metal cationUniRule annotation
    Metal bindingi10 – 101Divalent metal cationUniRule annotation
    Sitei36 – 361Transition state stabilizerUniRule annotation
    Metal bindingi44 – 441Divalent metal cationUniRule annotation
    Binding sitei67 – 671Substrate; via carbonyl oxygenUniRule annotation
    Sitei135 – 1351Transition state stabilizerUniRule annotation
    Binding sitei141 – 1411Substrate; via carbonyl oxygenUniRule annotation
    Binding sitei144 – 1441SubstrateUniRule annotation

    GO - Molecular functioni

    1. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway Source: UniProtKB-UniPathway
    2. terpenoid biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Isoprene biosynthesis

    Keywords - Ligandi

    Metal-binding

    Enzyme and pathway databases

    BioCyciYPES386656:GKD7-3063-MONOMER.
    UniPathwayiUPA00056; UER00095.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthaseUniRule annotation (EC:4.6.1.12UniRule annotation)
    Short name:
    MECDP-synthaseUniRule annotation
    Short name:
    MECPP-synthaseUniRule annotation
    Short name:
    MECPSUniRule annotation
    Gene namesi
    Name:ispFUniRule annotation
    Ordered Locus Names:YPDSF_3000
    OrganismiYersinia pestis (strain Pestoides F)
    Taxonomic identifieri386656 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
    ProteomesiUP000000236: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 1621622-C-methyl-D-erythritol 2,4-cyclodiphosphate synthasePRO_1000022895Add
    BLAST

    Interactioni

    Subunit structurei

    Homotrimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi386656.YPDSF_3000.

    Structurei

    3D structure databases

    ProteinModelPortaliA4TPZ8.
    SMRiA4TPZ8. Positions 1-157.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni8 – 103Substrate bindingUniRule annotation
    Regioni36 – 372Substrate bindingUniRule annotation
    Regioni40 – 489Substrate bindingUniRule annotation
    Regioni58 – 603Substrate bindingUniRule annotation
    Regioni63 – 675Substrate bindingUniRule annotation
    Regioni102 – 1087Substrate bindingUniRule annotation
    Regioni133 – 1375Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the IspF family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0245.
    HOGENOMiHOG000239175.
    KOiK01770.
    OMAiKGYQLGN.
    OrthoDBiEOG6J48RZ.

    Family and domain databases

    Gene3Di3.30.1330.50. 1 hit.
    HAMAPiMF_00107. IspF.
    InterProiIPR003526. MECDP_synthase.
    IPR020555. MECDP_synthase_CS.
    [Graphical view]
    PfamiPF02542. YgbB. 1 hit.
    [Graphical view]
    SUPFAMiSSF69765. SSF69765. 1 hit.
    TIGRFAMsiTIGR00151. ispF. 1 hit.
    PROSITEiPS01350. ISPF. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A4TPZ8-1 [UniParc]FASTAAdd to Basket

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    MRIGHGFDVH KFGENGSGPL IIGGVRIPYE KGLLAHSDGD VALHAATDAL    50
    LGAAALGDIG KLFPDTDPAF KGADSRGLLR EAYRRILAKG YKLGNLDITI 100
    IAQAPKMAPH IPQMRVNLAE DLQCHMDDIN VKATTTEQLG FTGRGEGIAC 150
    EAVVLLVNVE QG 162
    Length:162
    Mass (Da):17,182
    Last modified:May 15, 2007 - v1
    Checksum:i9A295411D3DFB1EB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000668 Genomic DNA. Translation: ABP41360.1.
    RefSeqiYP_001164333.1. NC_009381.1.

    Genome annotation databases

    EnsemblBacteriaiABP41360; ABP41360; YPDSF_3000.
    GeneIDi5062891.
    KEGGiypp:YPDSF_3000.
    PATRICi18615821. VBIYerPes122972_3789.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000668 Genomic DNA. Translation: ABP41360.1 .
    RefSeqi YP_001164333.1. NC_009381.1.

    3D structure databases

    ProteinModelPortali A4TPZ8.
    SMRi A4TPZ8. Positions 1-157.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 386656.YPDSF_3000.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABP41360 ; ABP41360 ; YPDSF_3000 .
    GeneIDi 5062891.
    KEGGi ypp:YPDSF_3000.
    PATRICi 18615821. VBIYerPes122972_3789.

    Phylogenomic databases

    eggNOGi COG0245.
    HOGENOMi HOG000239175.
    KOi K01770.
    OMAi KGYQLGN.
    OrthoDBi EOG6J48RZ.

    Enzyme and pathway databases

    UniPathwayi UPA00056 ; UER00095 .
    BioCyci YPES386656:GKD7-3063-MONOMER.

    Family and domain databases

    Gene3Di 3.30.1330.50. 1 hit.
    HAMAPi MF_00107. IspF.
    InterProi IPR003526. MECDP_synthase.
    IPR020555. MECDP_synthase_CS.
    [Graphical view ]
    Pfami PF02542. YgbB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF69765. SSF69765. 1 hit.
    TIGRFAMsi TIGR00151. ispF. 1 hit.
    PROSITEi PS01350. ISPF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Pestoides F.

    Entry informationi

    Entry nameiISPF_YERPP
    AccessioniPrimary (citable) accession number: A4TPZ8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: May 15, 2007
    Last modified: October 1, 2014
    This is version 55 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3