A4TE84 (RNH2_MYCGI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease HII
Short name=RNase HII
EC=3.1.26.4

Gene names

Name:	rnhB
Ordered Locus Names:	Mflv_4168

Organism

Mycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK)) [Complete proteome] [HAMAP]

Taxonomic identifier

350054 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that specifically degrades the RNA of RNA-DNA hybrids By similarity. HAMAP MF_00052_B
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP MF_00052_B
Cofactor	Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding By similarity.
Subcellular location	Cytoplasm Potential HAMAP MF_00052_B.
Sequence similarities	Belongs to the RNase HII family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease H activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 253	253	Ribonuclease HII HAMAP MF_00052_B		PRO_1000074926
Sites
	Metal binding	36	1	Divalent metal cation By similarity
	Metal binding	37	1	Divalent metal cation By similarity
	Metal binding	130	1	Divalent metal cation By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4TE84 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: 0C13A756DEFB1585	FASTA	253	26,991
	10 20 30 40 50 60 MPAVWPPRTV IRKSSGLRTL ESALYRAGLG PVAGVDEVGR GACAGPLVVA ACILGPNRLE 70 80 90 100 110 120 SLSALDDSKK LNESERERLF PLIRRYAVAF HVVFIPSVEV DRRGVHVANI EGMRRAVAGL 130 140 150 160 170 180 AVRPGYVLSD GFRVPGLPMP SLPVVGGDAA AACIAAASVL AKVSRDRLMV AMDQEHPGYG 190 200 210 220 230 240 FADHKGYSTR AHSAALNDLG PSTQHRFSFI NVRRLVVDGE PGQGGELECG KLAVDVPVDM 250 PVDRVLREGQ LSR

References

[1]

"Complete sequence of chromosome of Mycobacterium gilvum PYR-GCK." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Miller C., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PYR-GCK.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000656 Genomic DNA. Translation: ABP46637.1.
RefSeq	YP_001135425.1. NC_009338.1.
3D structure databases
ProteinModelPortal	A4TE84.
ModBase	Search...
Protein-protein interaction databases
STRING	A4TE84.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000025057; EBMYCP00000024719; EBMYCG00000025052.
GeneID	4975481.
GenomeReviews	Gene locus Mflv_4168 in contig CP000656_GR.
KEGG	mgi:Mflv_4168.
PATRIC	18036549. VBIMycGil17082_4245.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0164.
GeneTree	EBGT00050000016477.
HOGENOM	HBG584843.
OMA	RLGPTPI.
PhylomeDB	A4TE84.
ProtClustDB	PRK00015.
Enzyme and pathway databases
BioCyc	MGIL350054:MFLV_4168-MONOMER.
Family and domain databases
HAMAP	MF_00052_B. RNase_HII_B. [Tree]
InterPro	IPR022898. RNase_HII. IPR001352. RNase_HII/HIII. IPR024567. RNase_HII/HIII_dom. IPR012337. RNaseH-like_dom. [Graphical view]
KO	K03470.
PANTHER	PTHR10954. RNase_HII/HIII. 1 hit.
Pfam	PF01351. RNase_HII. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RNH2_MYCGI

Accession

Primary (citable) accession number: A4TE84

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 15, 2007
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program