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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Mycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei256ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:Mflv_4229
OrganismiMycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
Taxonomic identifieri350054 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium fortuitum complex
Proteomesi
  • UP000000232 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003309791 – 491Glutamate--tRNA ligaseAdd BLAST491

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi350054.Mflv_4229

Structurei

3D structure databases

ProteinModelPortaliA4TE16
SMRiA4TE16
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi9 – 19"HIGH" regionUniRule annotationAdd BLAST11
Motifi253 – 257"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252720
KOiK01885
OMAiAYYAFDT
OrthoDBiPOG091H021W

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
1.10.1160.10, 1 hit
1.10.8.70, 1 hit
3.40.50.620, 2 hits
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020752 aa-tRNA-synth_I_codon-bd_sub1
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR020061 Glu_tRNA_lig_a-bdl
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit

Sequencei

Sequence statusi: Complete.

A4TE16-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVRVRFCPS PTGTPHVGLI RTALFNWAYA RHTGGDFVFR IEDTDAQRDS
60 70 80 90 100
EESYLALLDA LRWLGMDWDE GPEVGGPYAP YRQSQRRDLY LDVIRRLLAA
110 120 130 140 150
GEAYEAFSTA EEVEARHLAA GRNPKLGYDN FDRDLTEEQR AAFRAEGRQP
160 170 180 190 200
VVRLRMPDAD MTWVDLVRGE TTFPVGSVPD FALTRGSGDP LYTLVNPVDD
210 220 230 240 250
ALMKITHVLR GEDLLPSTPR QLALYAALIR IGVTDMTPQF AHLPSVLGEG
260 270 280 290 300
NKKLSKRDPQ SNLFLHRERG FIPEGLLNYL ALLGWSIADD RDIFSVQEMV
310 320 330 340 350
AAFDVADVNS NPARFDQKKA DALNAEHIRR LDEAEFVRRL ADYFSTHGYD
360 370 380 390 400
TGLDDARFAE AAALVQTRIV VLGDAWDLLK FLDESSFALD EKSAGKELKD
410 420 430 440 450
TAVPVLTAAL AALKGVGEWT TAQIEDALKV ALIDGLELKP RKAFGPVRVA
460 470 480 490
VTGSSISPPL FESLELLGRD RSLERLRAGR DHAAAAVTMD A
Length:491
Mass (Da):54,453
Last modified:May 15, 2007 - v1
Checksum:iE09D2DECBE0E20CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000656 Genomic DNA Translation: ABP46698.1
RefSeqiWP_011895073.1, NC_009338.1

Genome annotation databases

EnsemblBacteriaiABP46698; ABP46698; Mflv_4229
KEGGimgi:Mflv_4229

Similar proteinsi

Entry informationi

Entry nameiSYE_MYCGI
AccessioniPrimary (citable) accession number: A4TE16
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: May 15, 2007
Last modified: May 23, 2018
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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