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A4TE16

- SYE_MYCGI

UniProt

A4TE16 - SYE_MYCGI

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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Mycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei256 – 2561ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMGIL350054:GHK8-4277-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:Mflv_4229
OrganismiMycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
Taxonomic identifieri350054 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000232: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 491491Glutamate--tRNA ligasePRO_0000330979Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi350054.Mflv_4229.

Structurei

3D structure databases

ProteinModelPortaliA4TE16.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi9 – 1911"HIGH" regionAdd
BLAST
Motifi253 – 2575"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK01885.
OMAiPEGMLNY.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

A4TE16-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKVRVRFCPS PTGTPHVGLI RTALFNWAYA RHTGGDFVFR IEDTDAQRDS
60 70 80 90 100
EESYLALLDA LRWLGMDWDE GPEVGGPYAP YRQSQRRDLY LDVIRRLLAA
110 120 130 140 150
GEAYEAFSTA EEVEARHLAA GRNPKLGYDN FDRDLTEEQR AAFRAEGRQP
160 170 180 190 200
VVRLRMPDAD MTWVDLVRGE TTFPVGSVPD FALTRGSGDP LYTLVNPVDD
210 220 230 240 250
ALMKITHVLR GEDLLPSTPR QLALYAALIR IGVTDMTPQF AHLPSVLGEG
260 270 280 290 300
NKKLSKRDPQ SNLFLHRERG FIPEGLLNYL ALLGWSIADD RDIFSVQEMV
310 320 330 340 350
AAFDVADVNS NPARFDQKKA DALNAEHIRR LDEAEFVRRL ADYFSTHGYD
360 370 380 390 400
TGLDDARFAE AAALVQTRIV VLGDAWDLLK FLDESSFALD EKSAGKELKD
410 420 430 440 450
TAVPVLTAAL AALKGVGEWT TAQIEDALKV ALIDGLELKP RKAFGPVRVA
460 470 480 490
VTGSSISPPL FESLELLGRD RSLERLRAGR DHAAAAVTMD A
Length:491
Mass (Da):54,453
Last modified:May 15, 2007 - v1
Checksum:iE09D2DECBE0E20CA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000656 Genomic DNA. Translation: ABP46698.1.
RefSeqiWP_011895073.1. NC_009338.1.
YP_001135486.1. NC_009338.1.

Genome annotation databases

EnsemblBacteriaiABP46698; ABP46698; Mflv_4229.
GeneIDi4976656.
KEGGimgi:Mflv_4229.
PATRICi18036681. VBIMycGil17082_4309.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000656 Genomic DNA. Translation: ABP46698.1 .
RefSeqi WP_011895073.1. NC_009338.1.
YP_001135486.1. NC_009338.1.

3D structure databases

ProteinModelPortali A4TE16.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 350054.Mflv_4229.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABP46698 ; ABP46698 ; Mflv_4229 .
GeneIDi 4976656.
KEGGi mgi:Mflv_4229.
PATRICi 18036681. VBIMycGil17082_4309.

Phylogenomic databases

eggNOGi COG0008.
HOGENOMi HOG000252720.
KOi K01885.
OMAi PEGMLNY.
OrthoDBi EOG6DRPF7.

Enzyme and pathway databases

BioCyci MGIL350054:GHK8-4277-MONOMER.

Family and domain databases

Gene3Di 1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPi MF_00022_B. Glu_tRNA_synth_B.
InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
PANTHERi PTHR10119. PTHR10119. 1 hit.
Pfami PF00749. tRNA-synt_1c. 1 hit.
[Graphical view ]
PRINTSi PR00987. TRNASYNTHGLU.
SUPFAMi SSF48163. SSF48163. 1 hit.
TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PYR-GCK.

Entry informationi

Entry nameiSYE_MYCGI
AccessioniPrimary (citable) accession number: A4TE16
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: May 15, 2007
Last modified: October 29, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3