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A4TE16

- SYE_MYCGI

UniProt

A4TE16 - SYE_MYCGI

Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Mycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 1 (15 May 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

    Catalytic activityi

    ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei256 – 2561ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
    3. tRNA binding Source: InterPro

    GO - Biological processi

    1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMGIL350054:GHK8-4277-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
    Alternative name(s):
    Glutamyl-tRNA synthetaseUniRule annotation
    Short name:
    GluRSUniRule annotation
    Gene namesi
    Name:gltXUniRule annotation
    Ordered Locus Names:Mflv_4229
    OrganismiMycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
    Taxonomic identifieri350054 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
    ProteomesiUP000000232: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 491491Glutamate--tRNA ligasePRO_0000330979Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi350054.Mflv_4229.

    Structurei

    3D structure databases

    ProteinModelPortaliA4TE16.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi9 – 1911"HIGH" regionAdd
    BLAST
    Motifi253 – 2575"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0008.
    HOGENOMiHOG000252720.
    KOiK01885.
    OMAiPEGMLNY.
    OrthoDBiEOG6DRPF7.

    Family and domain databases

    Gene3Di1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPiMF_00022_B. Glu_tRNA_synth_B.
    InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10119. PTHR10119. 1 hit.
    PfamiPF00749. tRNA-synt_1c. 1 hit.
    [Graphical view]
    PRINTSiPR00987. TRNASYNTHGLU.
    SUPFAMiSSF48163. SSF48163. 1 hit.
    TIGRFAMsiTIGR00464. gltX_bact. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A4TE16-1 [UniParc]FASTAAdd to Basket

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    MKVRVRFCPS PTGTPHVGLI RTALFNWAYA RHTGGDFVFR IEDTDAQRDS    50
    EESYLALLDA LRWLGMDWDE GPEVGGPYAP YRQSQRRDLY LDVIRRLLAA 100
    GEAYEAFSTA EEVEARHLAA GRNPKLGYDN FDRDLTEEQR AAFRAEGRQP 150
    VVRLRMPDAD MTWVDLVRGE TTFPVGSVPD FALTRGSGDP LYTLVNPVDD 200
    ALMKITHVLR GEDLLPSTPR QLALYAALIR IGVTDMTPQF AHLPSVLGEG 250
    NKKLSKRDPQ SNLFLHRERG FIPEGLLNYL ALLGWSIADD RDIFSVQEMV 300
    AAFDVADVNS NPARFDQKKA DALNAEHIRR LDEAEFVRRL ADYFSTHGYD 350
    TGLDDARFAE AAALVQTRIV VLGDAWDLLK FLDESSFALD EKSAGKELKD 400
    TAVPVLTAAL AALKGVGEWT TAQIEDALKV ALIDGLELKP RKAFGPVRVA 450
    VTGSSISPPL FESLELLGRD RSLERLRAGR DHAAAAVTMD A 491
    Length:491
    Mass (Da):54,453
    Last modified:May 15, 2007 - v1
    Checksum:iE09D2DECBE0E20CA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000656 Genomic DNA. Translation: ABP46698.1.
    RefSeqiWP_011895073.1. NC_009338.1.
    YP_001135486.1. NC_009338.1.

    Genome annotation databases

    EnsemblBacteriaiABP46698; ABP46698; Mflv_4229.
    GeneIDi4976656.
    KEGGimgi:Mflv_4229.
    PATRICi18036681. VBIMycGil17082_4309.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000656 Genomic DNA. Translation: ABP46698.1 .
    RefSeqi WP_011895073.1. NC_009338.1.
    YP_001135486.1. NC_009338.1.

    3D structure databases

    ProteinModelPortali A4TE16.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 350054.Mflv_4229.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABP46698 ; ABP46698 ; Mflv_4229 .
    GeneIDi 4976656.
    KEGGi mgi:Mflv_4229.
    PATRICi 18036681. VBIMycGil17082_4309.

    Phylogenomic databases

    eggNOGi COG0008.
    HOGENOMi HOG000252720.
    KOi K01885.
    OMAi PEGMLNY.
    OrthoDBi EOG6DRPF7.

    Enzyme and pathway databases

    BioCyci MGIL350054:GHK8-4277-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPi MF_00022_B. Glu_tRNA_synth_B.
    InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10119. PTHR10119. 1 hit.
    Pfami PF00749. tRNA-synt_1c. 1 hit.
    [Graphical view ]
    PRINTSi PR00987. TRNASYNTHGLU.
    SUPFAMi SSF48163. SSF48163. 1 hit.
    TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PYR-GCK.

    Entry informationi

    Entry nameiSYE_MYCGI
    AccessioniPrimary (citable) accession number: A4TE16
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: May 15, 2007
    Last modified: October 1, 2014
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3