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Protein

Deoxyuridine 5'-triphosphate nucleotidohydrolase

Gene

dut

Organism
Mycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.UniRule annotation

Miscellaneous

Each trimer binds three substrate molecules. The ligands are bound between subunits, and for each substrate molecule, residues from adjacent subunits contribute to the binding interactions (By similarity).By similarity

Catalytic activityi

dUTP + H2O = dUMP + diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: dUMP biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes dUMP from dCTP (dUTP route).UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut)
This subpathway is part of the pathway dUMP biosynthesis, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dUMP from dCTP (dUTP route), the pathway dUMP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei77SubstrateUniRule annotation1
Binding sitei91Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processNucleotide metabolism
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00610; UER00666

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolaseUniRule annotation (EC:3.6.1.23UniRule annotation)
Short name:
dUTPaseUniRule annotation
Alternative name(s):
dUTP pyrophosphataseUniRule annotation
Gene namesi
Name:dutUniRule annotation
Ordered Locus Names:Mflv_3933
OrganismiMycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
Taxonomic identifieri350054 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000232 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000760611 – 154Deoxyuridine 5'-triphosphate nucleotidohydrolaseAdd BLAST154

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi350054.Mflv_3933

Structurei

3D structure databases

ProteinModelPortaliA4TCR4
SMRiA4TCR4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni64 – 66Substrate bindingUniRule annotation3
Regioni81 – 83Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the dUTPase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107ZFB Bacteria
COG0756 LUCA
HOGENOMiHOG000028966
KOiK01520
OMAiGVILINH
OrthoDBiPOG091H01I6

Family and domain databases

CDDicd07557 trimeric_dUTPase, 1 hit
Gene3Di2.70.40.10, 1 hit
HAMAPiMF_00116 dUTPase_bact, 1 hit
InterProiView protein in InterPro
IPR029054 dUTPase-like
IPR036157 dUTPase-like_sf
IPR008181 dUTPase_1
IPR033704 dUTPase_trimeric
PfamiView protein in Pfam
PF00692 dUTPase, 1 hit
SUPFAMiSSF51283 SSF51283, 1 hit
TIGRFAMsiTIGR00576 dut, 1 hit

Sequencei

Sequence statusi: Complete.

A4TCR4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTSLAVVRL DPDLPLPSRA HDGDAGVDLY SAQDVELGPG ERALVPTGVA
60 70 80 90 100
VAIPHGMVGL IHPRSGLAAR VGLSIVNTPG TVDAGYRGEI KVCLINLDTS
110 120 130 140 150
TPITIGRGDR IAQLLVQRVE LPELVEVTSF DEAGLADTTR GDGGYGSSGG

HASL
Length:154
Mass (Da):15,818
Last modified:May 15, 2007 - v1
Checksum:i16BA33EBFEF32A5D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000656 Genomic DNA Translation: ABP46404.1
RefSeqiWP_011894784.1, NC_009338.1

Genome annotation databases

EnsemblBacteriaiABP46404; ABP46404; Mflv_3933
KEGGimgi:Mflv_3933

Similar proteinsi

Entry informationi

Entry nameiDUT_MYCGI
AccessioniPrimary (citable) accession number: A4TCR4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 15, 2007
Last modified: April 25, 2018
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health