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Reviewed, UniProtKB/Swiss-Prot A4TC13 (RIBBA_MYCGI)

Last modified November 3, 2009. Version 22. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Riboflavin biosynthesis protein ribBA
Including the following 2 domains:
    1- Recommended name:
            3,4-dihydroxy-2-butanone 4-phosphate synthase
                Short name=DHBP synthase
              EC=4.1.99.12
    2- Recommended name:
            GTP cyclohydrolase-2
              EC=3.5.4.25
        Alternative name(s):
            GTP cyclohydrolase II
Gene names
Name: ribBA
Ordered Locus Names: Mflv_3719
OrganismMycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK)) [Complete proteome] [HAMAP]
Taxonomic identifier350054 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

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Protein attributes

Sequence length423 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate By similarity.

Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate By similarity.

Catalytic activity

D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate. HAMAP MF_01283

GTP + 3 H2O = formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + diphosphate. HAMAP MF_01283

Cofactor

Binds 2 divalent metal cations per subunit. Magnesium or manganese By similarity.

Binds 1 zinc ion per subunit By similarity.

Pathway

Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1. HAMAP MF_01283

Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 1/4. HAMAP MF_01283

Sequence similarities

In the N-terminal section; belongs to the DHBP synthase family.

In the C-terminal section; belongs to the GTP cyclohydrolase II family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 423423Riboflavin biosynthesis protein ribBA HAMAP MF_01283
PRO_1000085898

Regions

Nucleotide binding259 – 2635GTP By similarity
Nucleotide binding303 – 3053GTP By similarity
Region1 – 204204DHBP synthase HAMAP MF_01283
Region28 – 292D-ribulose 5-phosphate binding By similarity
Region141 – 1455D-ribulose 5-phosphate binding By similarity
Region205 – 423219GTP cyclohydrolase II HAMAP MF_01283

Sites

Active site3371Proton acceptor; for GTP cyclohydrolase activity Potential
Active site3391Nucleophile; for GTP cyclohydrolase activity By similarity
Metal binding291Magnesium or manganese 1 By similarity
Metal binding291Magnesium or manganese 2 By similarity
Metal binding1441Magnesium or manganese 2 By similarity
Metal binding2641Zinc; catalytic By similarity
Metal binding2751Zinc; catalytic By similarity
Metal binding2771Zinc; catalytic By similarity
Binding site331D-ribulose 5-phosphate By similarity
Binding site1651D-ribulose 5-phosphate By similarity
Binding site2801GTP By similarity
Binding site3251GTP By similarity
Binding site3601GTP By similarity
Binding site3651GTP By similarity
Site1271Essential for DHBP synthase activity By similarity
Site1651Essential for DHBP synthase activity By similarity

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Sequences

Sequence LengthMass (Da)Tools
A4TC13-1 [UniParc].

Last modified May 15, 2007. Version 1.
Checksum: B5EE1BA334E51797

FASTA42345,682
        10         20         30         40         50         60 
MTRLDSVERA VADIAAGKAV VVIDDEDREN EGDLIFAAEK ATPELVAFMV RYTSGYLCVP 

        70         80         90        100        110        120 
LAGEICDRLG LLPMYAVNQD KHGTAYTVTV DAKVGVGTGI SASDRATTMR LLADPDSVAD 

       130        140        150        160        170        180 
DFTKPGHVVP LRAKDGGVLR RPGHTEAAVD LARLAGLQAA GTICEIVSQK DEGAMAQTDE 

       190        200        210        220        230        240 
LRIFADEHDL ALISIADLIE WRRKHEKHIA RVAEARIPTR HGEFRAVGYT SIYEDVEHVA 

       250        260        270        280        290        300 
LVKGDIAGPH GDGHDVLVRV HSECLTGDVF GSRRCDCGPQ LDAALAMVAR EGRGIVLYMR 

       310        320        330        340        350        360 
GHEGRGIGLM HKLQAYQLQD AGDDTVDANL KLGLPADARD YGTGAQILVD LGVRSMRLLT 

       370        380        390        400        410        420 
NNPAKRVGLD GYGLHIIERV PLPVRANAEN IRYLMTKRDR MGHDLVGLDD FDEAVPGEFG 


GAV

« Hide

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References

[1]"Complete sequence of chromosome of Mycobacterium gilvum PYR-GCK."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Mikhailova N., Miller C., Richardson P.
Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

CP000656 Genomic DNA. Translation: ABP46191.1.
RefSeqYP_001134979.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGA4TC13.

Genome annotation databases

GeneID4975035.
GenomeReviewsGene locus Mflv_3719 in contig CP000656_GR.
KEGGmgi:Mflv_3719.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMALRCDCRM.

Family and domain databases

HAMAPMF_01283.
[Tree]
InterProIPR017945. DHBP_synth_RibB-like_a/b_dom.
IPR000422. DHBP_synthase_RibB.
IPR000926. GTP_CycHdrlase_II.
IPR016299. Riboflavin_synth_RibA.
[Graphical view]
Gene3DG3DSA:3.90.870.10. DHBP_synth_RibB-like_a/b_dom. 1 hit.
PfamPF00926. DHBP_synthase. 1 hit.
PF00925. GTP_cyclohydro2. 1 hit.
[Graphical view]
PIRSFPIRSF001259. RibA. 1 hit.
ProDomPD003034. DHBP_synthase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR00505. ribA. 1 hit.
TIGR00506. ribB. 1 hit.
ProtoNetSearch...

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Entry information

Entry nameRIBBA_MYCGI
AccessionPrimary (citable) accession number: A4TC13
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 15, 2007
Last modified: November 3, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents