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Protein

L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase

Gene

mshC

Organism
Mycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent condensation of GlcN-Ins and L-cysteine to form L-Cys-GlcN-Ins.UniRule annotation

Catalytic activityi

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP = 1-O-(2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + AMP + diphosphate.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi75 – 751ZincUniRule annotation
Binding sitei90 – 901Cysteinyl adenylateUniRule annotation
Binding sitei259 – 2591Cysteinyl adenylateUniRule annotation
Metal bindingi263 – 2631ZincUniRule annotation
Metal bindingi288 – 2881ZincUniRule annotation
Binding sitei315 – 3151Cysteinyl adenylate; via amide nitrogen and carbonyl oxygenUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMGIL350054:GHK8-3085-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligaseUniRule annotation (EC:6.3.1.13UniRule annotation)
Short name:
L-Cys:GlcN-Ins ligaseUniRule annotation
Alternative name(s):
Mycothiol ligaseUniRule annotation
Short name:
MSH ligaseUniRule annotation
Gene namesi
Name:mshCUniRule annotation
Ordered Locus Names:Mflv_3049
OrganismiMycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
Taxonomic identifieri350054 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000232 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 444444L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligasePRO_0000400460Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi350054.Mflv_3049.

Structurei

3D structure databases

ProteinModelPortaliA4TB85.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni75 – 784Cysteinyl adenylate bindingUniRule annotation
Regioni113 – 1153Cysteinyl adenylate bindingUniRule annotation
Regioni281 – 2833Cysteinyl adenylate bindingUniRule annotation

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi77 – 8711"HIGH" regionUniRule annotationAdd
BLAST
Motifi219 – 2246"ERGGDP" regionUniRule annotation
Motifi321 – 3255"KMSKS" regionUniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. MshC subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C8N. Bacteria.
COG0215. LUCA.
HOGENOMiHOG000245251.
KOiK15526.
OMAiSGHDVHY.
OrthoDBiPOG091H004K.

Family and domain databases

CDDicd00672. CysRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01697. MshC. 1 hit.
InterProiIPR024909. Cys-tRNA/MSH_ligase.
IPR017812. Mycothiol_ligase_MshC.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
TIGRFAMsiTIGR03447. mycothiol_MshC. 1 hit.

Sequencei

Sequence statusi: Complete.

A4TB85-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPCDRKTSPD QHHALQIHRH HHRRKIRARV DAMQSWSAPS VPALPGRGPQ
60 70 80 90 100
LRLFDSADRQ IRPVSPGATA TMYVCGITPY DATHLGHAAT YLTFDLVNRV
110 120 130 140 150
WRDSGHDVHY VQNITDVDDP LFERAARDGI GWRELGDRET DLFREDMAAL
160 170 180 190 200
RVVPPRDYVA ATEAIAEVIE VVEKLLASGA AYVVDDAQYP DVYFHAGATV
210 220 230 240 250
QFGYESGYDR ETMLALFAER GGDPDRPGKS DPLDALLWRA ERPEEPSWPS
260 270 280 290 300
PFGPGRPGWH VECAAIAMTR IGNVLDIQGG GSDLIFPHHE FSSAHAECVS
310 320 330 340 350
GERRFARHYV HAGMIGWDGH KMSKSRGNLV LVSQLRRDGV DPGAIRLGLF
360 370 380 390 400
AGHYRQDRYW SPAVLDEAQQ RLHRWRSATA LDGAPDATDV VARVRRYLAD
410 420 430 440
DLDTPKALAA IDGWAEDTLS YGGHDRTAGP TVSAAVDALL GVAL
Length:444
Mass (Da):49,081
Last modified:May 15, 2007 - v1
Checksum:i6A3432B1C7AECC77
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000656 Genomic DNA. Translation: ABP45526.1.

Genome annotation databases

EnsemblBacteriaiABP45526; ABP45526; Mflv_3049.
KEGGimgi:Mflv_3049.
PATRICi18034246. VBIMycGil17082_3104.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000656 Genomic DNA. Translation: ABP45526.1.

3D structure databases

ProteinModelPortaliA4TB85.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi350054.Mflv_3049.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABP45526; ABP45526; Mflv_3049.
KEGGimgi:Mflv_3049.
PATRICi18034246. VBIMycGil17082_3104.

Phylogenomic databases

eggNOGiENOG4105C8N. Bacteria.
COG0215. LUCA.
HOGENOMiHOG000245251.
KOiK15526.
OMAiSGHDVHY.
OrthoDBiPOG091H004K.

Enzyme and pathway databases

BioCyciMGIL350054:GHK8-3085-MONOMER.

Family and domain databases

CDDicd00672. CysRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_01697. MshC. 1 hit.
InterProiIPR024909. Cys-tRNA/MSH_ligase.
IPR017812. Mycothiol_ligase_MshC.
IPR014729. Rossmann-like_a/b/a_fold.
IPR032678. tRNA-synt_1_cat_dom.
[Graphical view]
PANTHERiPTHR10890. PTHR10890. 1 hit.
PfamiPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSiPR00983. TRNASYNTHCYS.
TIGRFAMsiTIGR03447. mycothiol_MshC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMSHC_MYCGI
AccessioniPrimary (citable) accession number: A4TB85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: May 15, 2007
Last modified: September 7, 2016
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.