A4TAC4 (MASZ_MYCGI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate synthase G
EC=2.3.3.9

Gene names

Name:	glcB
Ordered Locus Names:	Mflv_3367

Organism

Mycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK)) [Complete proteome] [HAMAP]

Taxonomic identifier

350054 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	738 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP MF_00641
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP MF_00641
Subunit structure	Monomer By similarity. HAMAP MF_00641
Subcellular location	Cytoplasm By similarity HAMAP MF_00641.
Sequence similarities	Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 738	738	Malate synthase G HAMAP MF_00641		PRO_1000082700
Sites
	Active site	341	1	Proton acceptor By similarity
	Active site	643	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4TAC4 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: BC1F3D6E3DE24494	FASTA	738	80,045
	10 20 30 40 50 60 MTDRVTVGNL RVSPVLYDFI NNEALPGTDI DPDTFWSGVD KIVADLTPKN QDLLARRDHL 70 80 90 100 110 120 QAQIDKWHRA RVIGGFEAED YKQFLTEIGY LEPEPADFSV TTSGVDDEIT TTAGPQLVVP 130 140 150 160 170 180 ILNARFALNA ANARWGSLYD ALYGTDVISD EDGAEAGSSY NKRRGDKVIA YARRFLDGAA 190 200 210 220 230 240 PLASGSWTDI TGLRIDDGAV SATLDDGGSV ALADPQQFVG YHGEPDAPTA VLLANNGLHI 250 260 270 280 290 300 EILIDADSPI GSTDKAGIKD VVLESAITTI MDFEDSVAAV DADDKVLAYR NWLGLNRGDL 310 320 330 340 350 360 AEEVSKGGKT FTRVLNSDRT YRAPDGETEL TLPGRSLLFV RNVGHLMTND AIVVDDNGAD 370 380 390 400 410 420 GAEVPEGIQD ALFTSLIGIH GLRSAGDDDT KNGPLLNSRT GSIYIVKPKM HGPDEVAFTC 430 440 450 460 470 480 ELFSRVEDVL GLPQNTIKVG IMDEERRTTL NLKACIKAAA DRVVFINTGF LDRTGDEIHT 490 500 510 520 530 540 SMEAGPMIRK GAMKSTDWIA AYENQNVDIG LETGLSGKAQ IGKGMWAMTD LMADMVEQKI 550 560 570 580 590 600 GQPKAGATTA WVPSPTAATL HAMHYHQVDV FAVQSELAGK RRSTLDQLLT IPLATELAWA 610 620 630 640 650 660 PEEIREEVDN NCQSILGYVV RWIDAGVGCS KVPDIHDVAL MEDRATLRIS SQLLANWLRH 670 680 690 700 710 720 GVITEEDVKT SLRRMAAVVD EQNAADPDFR PMASDPDNSI AFQAAQELIL DGAAQPSGYT 730 EPILHRRRRE YKAASNVG

References

[1]

"Complete sequence of chromosome of Mycobacterium gilvum PYR-GCK." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Miller C., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PYR-GCK.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000656 Genomic DNA. Translation: ABP45843.1.
RefSeq	YP_001134631.1. NC_009338.1.
3D structure databases
ProteinModelPortal	A4TAC4.
SMR	A4TAC4. Positions 5-733.
ModBase	Search...
Protein-protein interaction databases
STRING	A4TAC4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000023849; EBMYCP00000023511; EBMYCG00000023844.
GeneID	4974687.
GenomeReviews	Gene locus Mflv_3367 in contig CP000656_GR.
KEGG	mgi:Mflv_3367.
PATRIC	18034880. VBIMycGil17082_3421.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2225.
GeneTree	EBGT00070000032288.
HOGENOM	HBG350005.
OMA	PKMHGPD.
PhylomeDB	A4TAC4.
ProtClustDB	PRK02999.
Enzyme and pathway databases
BioCyc	MGIL350054:MFLV_3367-MONOMER.
Family and domain databases
HAMAP	MF_00641. Malate_synth_G. [Tree]
InterPro	IPR011076. Malate_synth-like. IPR023310. Malate_synth_G_beta_sub_dom. IPR001465. Malate_synthase. IPR006253. Malate_synthG. [Graphical view]
Gene3D	G3DSA:2.170.170.11. Malate_synth_G_beta_sub_dom. 2 hits.
KO	K01638.
PANTHER	PTHR21631:SF1. Malate_synthase. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
SUPFAM	SSF51645. Malat_synth_like. 1 hit.
TIGRFAMs	TIGR01345. Malate_syn_G. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MASZ_MYCGI

Accession

Primary (citable) accession number: A4TAC4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 15, 2007
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program