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A4TAC4

- MASZ_MYCGI

UniProt

A4TAC4 - MASZ_MYCGI

Protein

Malate synthase G

Gene

glcB

Organism
Mycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (15 May 2007)
      Previous versions | rss
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    Functioni

    Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei118 – 1181Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Binding sitei276 – 2761Acetyl-CoAUniRule annotation
    Binding sitei313 – 3131Acetyl-CoAUniRule annotation
    Active sitei341 – 3411Proton acceptorUniRule annotation
    Binding sitei341 – 3411GlyoxylateUniRule annotation
    Metal bindingi444 – 4441MagnesiumUniRule annotation
    Binding sitei444 – 4441GlyoxylateUniRule annotation
    Metal bindingi472 – 4721MagnesiumUniRule annotation
    Binding sitei553 – 5531Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Active sitei643 – 6431Proton donorUniRule annotation

    GO - Molecular functioni

    1. malate synthase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-HAMAP
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciMGIL350054:GHK8-3403-MONOMER.
    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
    Gene namesi
    Name:glcBUniRule annotation
    Ordered Locus Names:Mflv_3367
    OrganismiMycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
    Taxonomic identifieri350054 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
    ProteomesiUP000000232: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 738738Malate synthase GPRO_1000082700Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei629 – 6291Cysteine sulfenic acid (-SOH)UniRule annotation

    Keywords - PTMi

    Oxidation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi350054.Mflv_3367.

    Structurei

    3D structure databases

    ProteinModelPortaliA4TAC4.
    SMRiA4TAC4. Positions 5-733.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni125 – 1262Acetyl-CoA bindingUniRule annotation
    Regioni469 – 4724Glyoxylate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2225.
    HOGENOMiHOG000220740.
    KOiK01638.
    OMAiSTIMDFE.
    OrthoDBiEOG6HJ286.

    Family and domain databases

    Gene3Di2.170.170.11. 2 hits.
    HAMAPiMF_00641. Malate_synth_G.
    InterProiIPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A4TAC4-1 [UniParc]FASTAAdd to Basket

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    MTDRVTVGNL RVSPVLYDFI NNEALPGTDI DPDTFWSGVD KIVADLTPKN    50
    QDLLARRDHL QAQIDKWHRA RVIGGFEAED YKQFLTEIGY LEPEPADFSV 100
    TTSGVDDEIT TTAGPQLVVP ILNARFALNA ANARWGSLYD ALYGTDVISD 150
    EDGAEAGSSY NKRRGDKVIA YARRFLDGAA PLASGSWTDI TGLRIDDGAV 200
    SATLDDGGSV ALADPQQFVG YHGEPDAPTA VLLANNGLHI EILIDADSPI 250
    GSTDKAGIKD VVLESAITTI MDFEDSVAAV DADDKVLAYR NWLGLNRGDL 300
    AEEVSKGGKT FTRVLNSDRT YRAPDGETEL TLPGRSLLFV RNVGHLMTND 350
    AIVVDDNGAD GAEVPEGIQD ALFTSLIGIH GLRSAGDDDT KNGPLLNSRT 400
    GSIYIVKPKM HGPDEVAFTC ELFSRVEDVL GLPQNTIKVG IMDEERRTTL 450
    NLKACIKAAA DRVVFINTGF LDRTGDEIHT SMEAGPMIRK GAMKSTDWIA 500
    AYENQNVDIG LETGLSGKAQ IGKGMWAMTD LMADMVEQKI GQPKAGATTA 550
    WVPSPTAATL HAMHYHQVDV FAVQSELAGK RRSTLDQLLT IPLATELAWA 600
    PEEIREEVDN NCQSILGYVV RWIDAGVGCS KVPDIHDVAL MEDRATLRIS 650
    SQLLANWLRH GVITEEDVKT SLRRMAAVVD EQNAADPDFR PMASDPDNSI 700
    AFQAAQELIL DGAAQPSGYT EPILHRRRRE YKAASNVG 738
    Length:738
    Mass (Da):80,045
    Last modified:May 15, 2007 - v1
    Checksum:iBC1F3D6E3DE24494
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000656 Genomic DNA. Translation: ABP45843.1.
    RefSeqiWP_011894227.1. NC_009338.1.
    YP_001134631.1. NC_009338.1.

    Genome annotation databases

    EnsemblBacteriaiABP45843; ABP45843; Mflv_3367.
    GeneIDi4974687.
    KEGGimgi:Mflv_3367.
    PATRICi18034880. VBIMycGil17082_3421.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000656 Genomic DNA. Translation: ABP45843.1 .
    RefSeqi WP_011894227.1. NC_009338.1.
    YP_001134631.1. NC_009338.1.

    3D structure databases

    ProteinModelPortali A4TAC4.
    SMRi A4TAC4. Positions 5-733.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 350054.Mflv_3367.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABP45843 ; ABP45843 ; Mflv_3367 .
    GeneIDi 4974687.
    KEGGi mgi:Mflv_3367.
    PATRICi 18034880. VBIMycGil17082_3421.

    Phylogenomic databases

    eggNOGi COG2225.
    HOGENOMi HOG000220740.
    KOi K01638.
    OMAi STIMDFE.
    OrthoDBi EOG6HJ286.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .
    BioCyci MGIL350054:GHK8-3403-MONOMER.

    Family and domain databases

    Gene3Di 2.170.170.11. 2 hits.
    HAMAPi MF_00641. Malate_synth_G.
    InterProi IPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PYR-GCK.

    Entry informationi

    Entry nameiMASZ_MYCGI
    AccessioniPrimary (citable) accession number: A4TAC4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: May 15, 2007
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3