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A4TAC4

- MASZ_MYCGI

UniProt

A4TAC4 - MASZ_MYCGI

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Protein
Malate synthase G
Gene
glcB, Mflv_3367
Organism
Mycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA By similarity.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei118 – 1181Acetyl-CoA; via carbonyl oxygen By similarity
Binding sitei276 – 2761Acetyl-CoA By similarity
Binding sitei313 – 3131Acetyl-CoA By similarity
Active sitei341 – 3411Proton acceptor By similarity
Binding sitei341 – 3411Glyoxylate By similarity
Metal bindingi444 – 4441Magnesium By similarity
Binding sitei444 – 4441Glyoxylate By similarity
Metal bindingi472 – 4721Magnesium By similarity
Binding sitei553 – 5531Acetyl-CoA; via carbonyl oxygen By similarity
Active sitei643 – 6431Proton donor By similarity

GO - Molecular functioni

  1. malate synthase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-HAMAP
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMGIL350054:GHK8-3403-MONOMER.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase G (EC:2.3.3.9)
Gene namesi
Name:glcB
Ordered Locus Names:Mflv_3367
OrganismiMycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
Taxonomic identifieri350054 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000232: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 738738Malate synthase GUniRule annotation
PRO_1000082700Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei629 – 6291Cysteine sulfenic acid (-SOH) By similarity

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi350054.Mflv_3367.

Structurei

3D structure databases

ProteinModelPortaliA4TAC4.
SMRiA4TAC4. Positions 5-733.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni125 – 1262Acetyl-CoA binding By similarity
Regioni469 – 4724Glyoxylate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiSTIMDFE.
OrthoDBiEOG6HJ286.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

A4TAC4-1 [UniParc]FASTAAdd to Basket

« Hide

MTDRVTVGNL RVSPVLYDFI NNEALPGTDI DPDTFWSGVD KIVADLTPKN    50
QDLLARRDHL QAQIDKWHRA RVIGGFEAED YKQFLTEIGY LEPEPADFSV 100
TTSGVDDEIT TTAGPQLVVP ILNARFALNA ANARWGSLYD ALYGTDVISD 150
EDGAEAGSSY NKRRGDKVIA YARRFLDGAA PLASGSWTDI TGLRIDDGAV 200
SATLDDGGSV ALADPQQFVG YHGEPDAPTA VLLANNGLHI EILIDADSPI 250
GSTDKAGIKD VVLESAITTI MDFEDSVAAV DADDKVLAYR NWLGLNRGDL 300
AEEVSKGGKT FTRVLNSDRT YRAPDGETEL TLPGRSLLFV RNVGHLMTND 350
AIVVDDNGAD GAEVPEGIQD ALFTSLIGIH GLRSAGDDDT KNGPLLNSRT 400
GSIYIVKPKM HGPDEVAFTC ELFSRVEDVL GLPQNTIKVG IMDEERRTTL 450
NLKACIKAAA DRVVFINTGF LDRTGDEIHT SMEAGPMIRK GAMKSTDWIA 500
AYENQNVDIG LETGLSGKAQ IGKGMWAMTD LMADMVEQKI GQPKAGATTA 550
WVPSPTAATL HAMHYHQVDV FAVQSELAGK RRSTLDQLLT IPLATELAWA 600
PEEIREEVDN NCQSILGYVV RWIDAGVGCS KVPDIHDVAL MEDRATLRIS 650
SQLLANWLRH GVITEEDVKT SLRRMAAVVD EQNAADPDFR PMASDPDNSI 700
AFQAAQELIL DGAAQPSGYT EPILHRRRRE YKAASNVG 738
Length:738
Mass (Da):80,045
Last modified:May 15, 2007 - v1
Checksum:iBC1F3D6E3DE24494
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000656 Genomic DNA. Translation: ABP45843.1.
RefSeqiWP_011894227.1. NC_009338.1.
YP_001134631.1. NC_009338.1.

Genome annotation databases

EnsemblBacteriaiABP45843; ABP45843; Mflv_3367.
GeneIDi4974687.
KEGGimgi:Mflv_3367.
PATRICi18034880. VBIMycGil17082_3421.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000656 Genomic DNA. Translation: ABP45843.1 .
RefSeqi WP_011894227.1. NC_009338.1.
YP_001134631.1. NC_009338.1.

3D structure databases

ProteinModelPortali A4TAC4.
SMRi A4TAC4. Positions 5-733.
ModBasei Search...

Protein-protein interaction databases

STRINGi 350054.Mflv_3367.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABP45843 ; ABP45843 ; Mflv_3367 .
GeneIDi 4974687.
KEGGi mgi:Mflv_3367.
PATRICi 18034880. VBIMycGil17082_3421.

Phylogenomic databases

eggNOGi COG2225.
HOGENOMi HOG000220740.
KOi K01638.
OMAi STIMDFE.
OrthoDBi EOG6HJ286.

Enzyme and pathway databases

UniPathwayi UPA00703 ; UER00720 .
BioCyci MGIL350054:GHK8-3403-MONOMER.

Family and domain databases

Gene3Di 2.170.170.11. 2 hits.
HAMAPi MF_00641. Malate_synth_G.
InterProi IPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view ]
Pfami PF01274. Malate_synthase. 1 hit.
[Graphical view ]
SUPFAMi SSF51645. SSF51645. 1 hit.
TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PYR-GCK.

Entry informationi

Entry nameiMASZ_MYCGI
AccessioniPrimary (citable) accession number: A4TAC4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 15, 2007
Last modified: September 3, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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