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A4TAC4

- MASZ_MYCGI

UniProt

A4TAC4 - MASZ_MYCGI

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Protein

Malate synthase G

Gene

glcB

Organism
Mycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei118 – 1181Acetyl-CoA; via carbonyl oxygenUniRule annotation
Binding sitei276 – 2761Acetyl-CoAUniRule annotation
Binding sitei313 – 3131Acetyl-CoAUniRule annotation
Active sitei341 – 3411Proton acceptorUniRule annotation
Binding sitei341 – 3411GlyoxylateUniRule annotation
Metal bindingi444 – 4441MagnesiumUniRule annotation
Binding sitei444 – 4441GlyoxylateUniRule annotation
Metal bindingi472 – 4721MagnesiumUniRule annotation
Binding sitei553 – 5531Acetyl-CoA; via carbonyl oxygenUniRule annotation
Active sitei643 – 6431Proton donorUniRule annotation

GO - Molecular functioni

  1. malate synthase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-HAMAP
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMGIL350054:GHK8-3403-MONOMER.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:Mflv_3367
OrganismiMycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
Taxonomic identifieri350054 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000232: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 738738Malate synthase GPRO_1000082700Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei629 – 6291Cysteine sulfenic acid (-SOH)UniRule annotation

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi350054.Mflv_3367.

Structurei

3D structure databases

ProteinModelPortaliA4TAC4.
SMRiA4TAC4. Positions 5-733.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni125 – 1262Acetyl-CoA bindingUniRule annotation
Regioni469 – 4724Glyoxylate bindingUniRule annotation

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiSTIMDFE.
OrthoDBiEOG6HJ286.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

A4TAC4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTDRVTVGNL RVSPVLYDFI NNEALPGTDI DPDTFWSGVD KIVADLTPKN
60 70 80 90 100
QDLLARRDHL QAQIDKWHRA RVIGGFEAED YKQFLTEIGY LEPEPADFSV
110 120 130 140 150
TTSGVDDEIT TTAGPQLVVP ILNARFALNA ANARWGSLYD ALYGTDVISD
160 170 180 190 200
EDGAEAGSSY NKRRGDKVIA YARRFLDGAA PLASGSWTDI TGLRIDDGAV
210 220 230 240 250
SATLDDGGSV ALADPQQFVG YHGEPDAPTA VLLANNGLHI EILIDADSPI
260 270 280 290 300
GSTDKAGIKD VVLESAITTI MDFEDSVAAV DADDKVLAYR NWLGLNRGDL
310 320 330 340 350
AEEVSKGGKT FTRVLNSDRT YRAPDGETEL TLPGRSLLFV RNVGHLMTND
360 370 380 390 400
AIVVDDNGAD GAEVPEGIQD ALFTSLIGIH GLRSAGDDDT KNGPLLNSRT
410 420 430 440 450
GSIYIVKPKM HGPDEVAFTC ELFSRVEDVL GLPQNTIKVG IMDEERRTTL
460 470 480 490 500
NLKACIKAAA DRVVFINTGF LDRTGDEIHT SMEAGPMIRK GAMKSTDWIA
510 520 530 540 550
AYENQNVDIG LETGLSGKAQ IGKGMWAMTD LMADMVEQKI GQPKAGATTA
560 570 580 590 600
WVPSPTAATL HAMHYHQVDV FAVQSELAGK RRSTLDQLLT IPLATELAWA
610 620 630 640 650
PEEIREEVDN NCQSILGYVV RWIDAGVGCS KVPDIHDVAL MEDRATLRIS
660 670 680 690 700
SQLLANWLRH GVITEEDVKT SLRRMAAVVD EQNAADPDFR PMASDPDNSI
710 720 730
AFQAAQELIL DGAAQPSGYT EPILHRRRRE YKAASNVG
Length:738
Mass (Da):80,045
Last modified:May 15, 2007 - v1
Checksum:iBC1F3D6E3DE24494
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000656 Genomic DNA. Translation: ABP45843.1.
RefSeqiYP_001134631.1. NC_009338.1.

Genome annotation databases

EnsemblBacteriaiABP45843; ABP45843; Mflv_3367.
GeneIDi4974687.
KEGGimgi:Mflv_3367.
PATRICi18034880. VBIMycGil17082_3421.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000656 Genomic DNA. Translation: ABP45843.1 .
RefSeqi YP_001134631.1. NC_009338.1.

3D structure databases

ProteinModelPortali A4TAC4.
SMRi A4TAC4. Positions 5-733.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 350054.Mflv_3367.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABP45843 ; ABP45843 ; Mflv_3367 .
GeneIDi 4974687.
KEGGi mgi:Mflv_3367.
PATRICi 18034880. VBIMycGil17082_3421.

Phylogenomic databases

eggNOGi COG2225.
HOGENOMi HOG000220740.
KOi K01638.
OMAi STIMDFE.
OrthoDBi EOG6HJ286.

Enzyme and pathway databases

UniPathwayi UPA00703 ; UER00720 .
BioCyci MGIL350054:GHK8-3403-MONOMER.

Family and domain databases

Gene3Di 2.170.170.11. 2 hits.
HAMAPi MF_00641. Malate_synth_G.
InterProi IPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view ]
Pfami PF01274. Malate_synthase. 1 hit.
[Graphical view ]
SUPFAMi SSF51645. SSF51645. 1 hit.
TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PYR-GCK.

Entry informationi

Entry nameiMASZ_MYCGI
AccessioniPrimary (citable) accession number: A4TAC4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 15, 2007
Last modified: November 26, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3