A4TA35 (KATG_MYCGI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	Mflv_3451

Organism

Mycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK)) [Complete proteome] [HAMAP]

Taxonomic identifier

350054 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	748 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 748	748	Catalase-peroxidase HAMAP MF_01961		PRO_0000354837
Sites
	Active site	113	1	Proton acceptor By similarity
	Metal binding	280	1	Iron (heme axial ligand) By similarity
	Site	109	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	112 ↔ 239		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-265) By similarity
	Cross-link	239 ↔ 265		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-112) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4TA35 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: 4576A542A1AD523E	FASTA	748	82,278
	10 20 30 40 50 60 MTDTSDARPP HSDAKTASNS ESENPAIDSP EPKSHAPLTN RDWWPEQVDV SVLHKQNEKG 70 80 90 100 110 120 NPFGEDFDYA AEFAKLDVDA FKADVIDLIR TSQDWWPADY GNYAGLFVRM SWHAAGTYRI 130 140 150 160 170 180 FDGRGGAGQG SQRFAPLNSW PDNANLDKAR RLLWPIKQKY GNKISWADLI AYAGNAALEQ 190 200 210 220 230 240 SGFKTAGFAF GREDIWEPEE MLWGQEDTWL GTDKRYGGSN DDKRELAEPF GATTMGLIYV 250 260 270 280 290 300 NPEGPEGKPD PLAAAHDIRE TFGRMAMNDE ETAALIVGGH TLGKTHGAAD VNVGPEPEGA 310 320 330 340 350 360 PMEQQGLGWK CPFGTGNGND TVTSGLEVIW TGTNSQWSNA FLENLYGNEW ELTKSPAGAW 370 380 390 400 410 420 QFEAKNAEAT IPDPFGGPPR KPTMLVTDVA MREDPIYGQI TRRWLDHPEE MDAAFAKAWF 430 440 450 460 470 480 KLMHRDMGPV SRYLGPWVPA EQEIWQDPVP AVDHALIDES DIAALKSQVL QSGLSVPQLV 490 500 510 520 530 540 KTAWASAASF RGTDKRGGAN GARLRLEPQR NWEANEPAEL DKVLPVLEKI QQDFNSTATG 550 560 570 580 590 600 GKKVSLADVI VLAGSAAVEK AAKDGGYEIT VHFAPGRTDA TQESTDVESF AVLEPRTDGF 610 620 630 640 650 660 RNFFRPGDKN PLEQQLVERA YLLDLTAPEL TALIGGLRAL GANHGGSKHG VFTDKPGVLS 670 680 690 700 710 720 NDFFLNLLDM RTEWKPSELS ENVFDGKDRA TGETKWTATA NDLVFGSNSV LRALAEVYAQ 730 740 DDNQGKFVED FVAAWVKVMN NDRFDLKQ

References

[1]

"Complete sequence of chromosome of Mycobacterium gilvum PYR-GCK." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Miller C., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PYR-GCK.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000656 Genomic DNA. Translation: ABP45926.1.
RefSeq	YP_001134714.1. NC_009338.1.
3D structure databases
ProteinModelPortal	A4TA35.
SMR	A4TA35. Positions 40-747.
ModBase	Search...
Protein-protein interaction databases
STRING	A4TA35.
Protein family/group databases
PeroxiBase	3622. MgiCP01_PYR-GCK.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000025807; EBMYCP00000025469; EBMYCG00000025802.
GeneID	4974770.
GenomeReviews	Gene locus Mflv_3451 in contig CP000656_GR.
KEGG	mgi:Mflv_3451.
PATRIC	18035054. VBIMycGil17082_3508.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0376.
GeneTree	EBGT00050000017549.
HOGENOM	HBG285610.
OMA	QGKFVED.
PhylomeDB	A4TA35.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	MGIL350054:MFLV_3451-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_MYCGI

Accession

Primary (citable) accession number: A4TA35

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	May 15, 2007
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program