A4T9V2 (SYFA_MYCGI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase alpha chain
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase alpha chain
Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	Mflv_3533

Organism

Mycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK)) [Complete proteome] [HAMAP]

Taxonomic identifier

350054 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00281
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_00281.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	Phenylalanine--tRNA ligase alpha chain HAMAP MF_00281		PRO_1000078844
Sites
	Metal binding	265	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	A4T9V2 [UniParc]. Last modified May 15, 2007. Version 1. Checksum: 9F7AB60DC8FC561B	FASTA	347	38,134
	10 20 30 40 50 60 MAEQPVDLSD QALADAVSAV RRAFDGASTL EDLAKAKTEY LGDRSPIALA RQALGSLPKT 70 80 90 100 110 120 ERADAGKRVN VARTEAQTAY DERLAVLRAE RDAAVLVAER IDVTLPSTRQ PVGARHPITI 130 140 150 160 170 180 LAERVADTFV AMGWELAEGP EVETEQFNFD ALNFPPDHPA RSEQDTFQVA PDGSRQVLRT 190 200 210 220 230 240 HTSPVQIRAL LERELPVYIV SIGRTFRTDE LDATHTPVFH QVEGLAVDKG LTMANLRGTL 250 260 270 280 290 300 DAFARSEFGP QGRTRFRPHF FPFTEPSAEV DIWFPDKKGG PGWVEWGGCG MVNPNVLRAC 310 320 330 340 GIDPEVYSGF AFGMGLERTL QFRNGIPDMR DMVEGDVRFS LPFGVGA

References

[1]

"Complete sequence of chromosome of Mycobacterium gilvum PYR-GCK." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Mikhailova N., Miller C., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PYR-GCK.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000656 Genomic DNA. Translation: ABP46007.1.
RefSeq	YP_001134795.1. NC_009338.1.
3D structure databases
ProteinModelPortal	A4T9V2.
ModBase	Search...
Protein-protein interaction databases
STRING	A4T9V2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000027282; EBMYCP00000026944; EBMYCG00000027277.
GeneID	4974851.
GenomeReviews	Gene locus Mflv_3533 in contig CP000656_GR.
KEGG	mgi:Mflv_3533.
PATRIC	18035230. VBIMycGil17082_3592.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0016.
GeneTree	EBGT00050000016426.
HOGENOM	HBG284353.
OMA	FRASYFP.
PhylomeDB	A4T9V2.
ProtClustDB	PRK00488.
Enzyme and pathway databases
BioCyc	MGIL350054:MFLV_3533-MONOMER.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR022911. Phe_tRNA_synth_alpha1_bac. IPR002319. Phenylalanyl-tRNA_Synthase. IPR010978. tRNA-bd_arm. [Graphical view]
KO	K01889.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
SUPFAM	SSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMs	TIGR00468. PheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_MYCGI

Accession

Primary (citable) accession number: A4T9V2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 15, 2007
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program