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Protein

8-amino-7-oxononanoate synthase

Gene

Mflv_3628

Organism
Mycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the decarboxylative condensation of pimeloyl-[acyl-carrier protein] and L-alanine to produce 8-amino-7-oxononanoate (AON), [acyl-carrier protein], and carbon dioxide.By similarity

Catalytic activityi

Pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein].

Cofactori

Pathwayi: biotin biosynthesis

This protein is involved in the pathway biotin biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei26 – 261SubstrateBy similarity
Binding sitei129 – 1291SubstrateBy similarity
Binding sitei175 – 1751Pyridoxal phosphateBy similarity
Binding sitei345 – 3451SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciMGIL350054:GHK8-3670-MONOMER.
UniPathwayiUPA00078.

Names & Taxonomyi

Protein namesi
Recommended name:
8-amino-7-oxononanoate synthase (EC:2.3.1.47)
Short name:
AONS
Alternative name(s):
7-keto-8-amino-pelargonic acid synthase
Short name:
7-KAP synthase
Short name:
KAPA synthase
8-amino-7-ketopelargonate synthase
Alpha-oxoamine synthase
Gene namesi
Ordered Locus Names:Mflv_3628
OrganismiMycobacterium gilvum (strain PYR-GCK) (Mycobacterium flavescens (strain ATCC 700033 / PYR-GCK))
Taxonomic identifieri350054 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000232 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3803808-amino-7-oxononanoate synthasePRO_0000381035Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei235 – 2351N6-(pyridoxal phosphate)lysineBy similarity

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi350054.Mflv_3628.

Structurei

3D structure databases

ProteinModelPortaliA4T9L3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni104 – 1052Pyridoxal phosphate bindingBy similarity
Regioni200 – 2034Pyridoxal phosphate bindingBy similarity
Regioni232 – 2354Pyridoxal phosphate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107EEK. Bacteria.
COG0156. LUCA.
HOGENOMiHOG000221021.
KOiK00652.
OMAiELAHACI.
OrthoDBiPOG091H024U.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A4T9L3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRAGLSPLA WLDEVETQRR AAGLRRSLRA RPPVGTVLDL ASNDYLGLSQ
60 70 80 90 100
HPRVIDGGVA ALRTWGAGAT GSRLVTGNTE LHEQFEDALA SFVGADSALV
110 120 130 140 150
FSSGYTANLG AVVALSGPGS LLVSDAYTHA SLVDACRLSR ARVVVTPHND
160 170 180 190 200
VAAVARALAT RDEERAVVVT DSVFSADGDL APLRELHDAC RRHGALMIVD
210 220 230 240 250
EAHGLGVRGD RGRGLLDEVG LAGAPDVVMT TTLSKALGSQ GGVVLGPAAV
260 270 280 290 300
RDHLIDAARP FIFDTGLAPA AVGAAHAALQ VLVEEPWRAQ RVLDHATTLA
310 320 330 340 350
QICGVADVPS SAVVSVILGE PEVALAAAMG CLERGVRVGC FRPPTVPAGT
360 370 380
SRLRLTARAS LSDDEMALAR EVLTDVLSRA
Length:380
Mass (Da):39,420
Last modified:May 15, 2007 - v1
Checksum:iC4C081B31E7BEB25
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000656 Genomic DNA. Translation: ABP46102.1.
RefSeqiWP_011894485.1. NC_009338.1.

Genome annotation databases

EnsemblBacteriaiABP46102; ABP46102; Mflv_3628.
KEGGimgi:Mflv_3628.
PATRICi18035426. VBIMycGil17082_3688.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000656 Genomic DNA. Translation: ABP46102.1.
RefSeqiWP_011894485.1. NC_009338.1.

3D structure databases

ProteinModelPortaliA4T9L3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi350054.Mflv_3628.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABP46102; ABP46102; Mflv_3628.
KEGGimgi:Mflv_3628.
PATRICi18035426. VBIMycGil17082_3688.

Phylogenomic databases

eggNOGiENOG4107EEK. Bacteria.
COG0156. LUCA.
HOGENOMiHOG000221021.
KOiK00652.
OMAiELAHACI.
OrthoDBiPOG091H024U.

Enzyme and pathway databases

UniPathwayiUPA00078.
BioCyciMGIL350054:GHK8-3670-MONOMER.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBIOF_MYCGI
AccessioniPrimary (citable) accession number: A4T9L3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 15, 2007
Last modified: September 7, 2016
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.